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Evaluation of eNOS gene polymorphisms in relation to BMD in postmenopausal women.
Maturitas. 2009 Aug 20; 63(4):352-6.M

Abstract

OBJECTIVE

The aim of the present study was to evaluate the relations between T(-786)C and Glu298Asp polymorphisms of the endothelial nitric oxide synthase (eNOS) gene and BMD in postmenopausal Turkish women.

METHODS

The T(-786)C and Glu298Asp polymorphisms were genotyped by PCR-RFLP method in 311 postmenopausal osteoporotic women (OP) and in 305 age-matched postmenopausal females (CG) with normal BMD.

RESULTS

None of the SNPs of the eNOS gene was significantly associated with BMD at the lumbar spine, femoral neck, Ward's triangle and femoral trochanter in the combined group. Mean BMD values were therefore found to be similar across the genotypes in postmenopausal Turkish women. However, there was a significant association between the T(-786)C polymorphism and BMD values at the lumbar spine in the normal control group (P=0.005), and at the femoral trochanter in the osteoporotic patients (P=0.046). The mean value of the lumbar spine BMD in the normal controls was significantly higher in women with the TC genotype of the T(-786)C polymorphism than in women with the TT genotype (P=0.0012). Women with the CC genotype of the T(-786)C polymorphism in the osteoporotic patients had significantly higher BMD value at the femoral trochanter than those with the TC (P=0.018) and TT genotypes (P=0.024). Frequencies of the TC heterozygotes for T(-786)C polymorphism were significantly higher among osteoporotic subjects than normal controls. Also, the CC and TT genotype frequencies of control group were significantly higher than those of the osteoporotic group at the femoral neck.

CONCLUSIONS

We conclude that, although the biological role of the nitric oxide synthases is well established, our study does not suggest that eNOS gene polymorphisms, T(-786)C and Glu298Asp, are major contributors to adult bone mineral density in the postmenopausal Turkish women.

Authors+Show Affiliations

Akdeniz University, Faculty of Medicine, Department of Physical Medicine and Rehabilitation, Antalya, Turkey. scubukcu@akdeniz.edu.trNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19520527

Citation

Firat, Sibel Cubukcu, et al. "Evaluation of eNOS Gene Polymorphisms in Relation to BMD in Postmenopausal Women." Maturitas, vol. 63, no. 4, 2009, pp. 352-6.
Firat SC, Cetin Z, Samanci N, et al. Evaluation of eNOS gene polymorphisms in relation to BMD in postmenopausal women. Maturitas. 2009;63(4):352-6.
Firat, S. C., Cetin, Z., Samanci, N., Aydin, F., Balci, N., Gungor, F., Firat, M. Z., Luleci, G., & Karauzum, S. B. (2009). Evaluation of eNOS gene polymorphisms in relation to BMD in postmenopausal women. Maturitas, 63(4), 352-6. https://doi.org/10.1016/j.maturitas.2009.05.004
Firat SC, et al. Evaluation of eNOS Gene Polymorphisms in Relation to BMD in Postmenopausal Women. Maturitas. 2009 Aug 20;63(4):352-6. PubMed PMID: 19520527.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Evaluation of eNOS gene polymorphisms in relation to BMD in postmenopausal women. AU - Firat,Sibel Cubukcu, AU - Cetin,Zafer, AU - Samanci,Nehir, AU - Aydin,Funda, AU - Balci,Nilufer, AU - Gungor,Firat, AU - Firat,Mehmet Ziya, AU - Luleci,Guven, AU - Karauzum,Sibel Berker, Y1 - 2009/06/10/ PY - 2009/03/07/received PY - 2009/05/09/revised PY - 2009/05/12/accepted PY - 2009/6/13/entrez PY - 2009/6/13/pubmed PY - 2009/11/6/medline SP - 352 EP - 6 JF - Maturitas JO - Maturitas VL - 63 IS - 4 N2 - OBJECTIVE: The aim of the present study was to evaluate the relations between T(-786)C and Glu298Asp polymorphisms of the endothelial nitric oxide synthase (eNOS) gene and BMD in postmenopausal Turkish women. METHODS: The T(-786)C and Glu298Asp polymorphisms were genotyped by PCR-RFLP method in 311 postmenopausal osteoporotic women (OP) and in 305 age-matched postmenopausal females (CG) with normal BMD. RESULTS: None of the SNPs of the eNOS gene was significantly associated with BMD at the lumbar spine, femoral neck, Ward's triangle and femoral trochanter in the combined group. Mean BMD values were therefore found to be similar across the genotypes in postmenopausal Turkish women. However, there was a significant association between the T(-786)C polymorphism and BMD values at the lumbar spine in the normal control group (P=0.005), and at the femoral trochanter in the osteoporotic patients (P=0.046). The mean value of the lumbar spine BMD in the normal controls was significantly higher in women with the TC genotype of the T(-786)C polymorphism than in women with the TT genotype (P=0.0012). Women with the CC genotype of the T(-786)C polymorphism in the osteoporotic patients had significantly higher BMD value at the femoral trochanter than those with the TC (P=0.018) and TT genotypes (P=0.024). Frequencies of the TC heterozygotes for T(-786)C polymorphism were significantly higher among osteoporotic subjects than normal controls. Also, the CC and TT genotype frequencies of control group were significantly higher than those of the osteoporotic group at the femoral neck. CONCLUSIONS: We conclude that, although the biological role of the nitric oxide synthases is well established, our study does not suggest that eNOS gene polymorphisms, T(-786)C and Glu298Asp, are major contributors to adult bone mineral density in the postmenopausal Turkish women. SN - 1873-4111 UR - https://www.unboundmedicine.com/medline/citation/19520527/Evaluation_of_eNOS_gene_polymorphisms_in_relation_to_BMD_in_postmenopausal_women_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-5122(09)00175-3 DB - PRIME DP - Unbound Medicine ER -