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Metabolite fingerprinting of Camptotheca acuminata and the HPLC-ESI-MS/MS analysis of camptothecin and related alkaloids.
J Pharm Biomed Anal. 2010 Jan 20; 51(2):405-15.JP

Abstract

The major phytochemical constituents, namely, alkaloids, flavonoids and ellagic acid derivatives, of leaves of Camptotheca acuminata were identified using high performance liquid chromatography (HPLC) coupled with electrospray mass spectrometry (ESI-MS) in extracts of plants cultivated in Italy and collected at different growth stages. Alkaloids related to camptothecin were identified and quantified by HPLC coupled with ESI-tandem mass spectrometry (MS/MS) employing, respectively, an ion trap and a triple quadrupole mass analyser. The fragmentation patterns of alkaloids related to camptothecin were analysed and a specific Multiple Reaction Monitoring HPLC-MS/MS method was developed for the quantitative determination of these constituents. The described method provides high sensitivity and specificity for the characterisation and quantitative determination of the alkaloids in C. acuminata.

Authors+Show Affiliations

Dipartimento di Scienze Farmaceutiche, Università degli Studi di Salerno, via Ponte don Melillo, 84084 Fisciano (SA), Italy. pmontoro@unisa.itNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19520541

Citation

Montoro, Paola, et al. "Metabolite Fingerprinting of Camptotheca Acuminata and the HPLC-ESI-MS/MS Analysis of Camptothecin and Related Alkaloids." Journal of Pharmaceutical and Biomedical Analysis, vol. 51, no. 2, 2010, pp. 405-15.
Montoro P, Maldini M, Piacente S, et al. Metabolite fingerprinting of Camptotheca acuminata and the HPLC-ESI-MS/MS analysis of camptothecin and related alkaloids. J Pharm Biomed Anal. 2010;51(2):405-15.
Montoro, P., Maldini, M., Piacente, S., Macchia, M., & Pizza, C. (2010). Metabolite fingerprinting of Camptotheca acuminata and the HPLC-ESI-MS/MS analysis of camptothecin and related alkaloids. Journal of Pharmaceutical and Biomedical Analysis, 51(2), 405-15. https://doi.org/10.1016/j.jpba.2009.05.013
Montoro P, et al. Metabolite Fingerprinting of Camptotheca Acuminata and the HPLC-ESI-MS/MS Analysis of Camptothecin and Related Alkaloids. J Pharm Biomed Anal. 2010 Jan 20;51(2):405-15. PubMed PMID: 19520541.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Metabolite fingerprinting of Camptotheca acuminata and the HPLC-ESI-MS/MS analysis of camptothecin and related alkaloids. AU - Montoro,Paola, AU - Maldini,Mariateresa, AU - Piacente,Sonia, AU - Macchia,Mario, AU - Pizza,Cosimo, Y1 - 2009/05/22/ PY - 2009/01/29/received PY - 2009/05/07/revised PY - 2009/05/08/accepted PY - 2009/6/13/entrez PY - 2009/6/13/pubmed PY - 2010/2/2/medline SP - 405 EP - 15 JF - Journal of pharmaceutical and biomedical analysis JO - J Pharm Biomed Anal VL - 51 IS - 2 N2 - The major phytochemical constituents, namely, alkaloids, flavonoids and ellagic acid derivatives, of leaves of Camptotheca acuminata were identified using high performance liquid chromatography (HPLC) coupled with electrospray mass spectrometry (ESI-MS) in extracts of plants cultivated in Italy and collected at different growth stages. Alkaloids related to camptothecin were identified and quantified by HPLC coupled with ESI-tandem mass spectrometry (MS/MS) employing, respectively, an ion trap and a triple quadrupole mass analyser. The fragmentation patterns of alkaloids related to camptothecin were analysed and a specific Multiple Reaction Monitoring HPLC-MS/MS method was developed for the quantitative determination of these constituents. The described method provides high sensitivity and specificity for the characterisation and quantitative determination of the alkaloids in C. acuminata. SN - 1873-264X UR - https://www.unboundmedicine.com/medline/citation/19520541/Metabolite_fingerprinting_of_Camptotheca_acuminata_and_the_HPLC_ESI_MS/MS_analysis_of_camptothecin_and_related_alkaloids_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0731-7085(09)00282-9 DB - PRIME DP - Unbound Medicine ER -