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Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn.
Methods Mol Biol. 2009; 547:205-15.MM

Abstract

We have established an efficient protocol for plant regeneration and production of secondary metabolites in hairy root culture of Centaurium erythraea Rafn. Because the hairy roots and regenerated plants produce bitter secoiridoid glucosides and xanthones similar to the plants in nature, the use of in vitro cultures as an alternative source of their production is feasible. This chapter describes a protocol for the induction of adventitious shoots and transgenic plants from hairy root cultures of C. erythraea and their phytochemical analysis.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Subotić A
Institute for Biological Research Sinisa Stankovic, University of Belgrade, Belgrade, Serbia.
Jevremović S
No affiliation info available
Grubisić D
No affiliation info available
Janković T
No affiliation info available

MeSH

CentauriumChromatography, High Pressure LiquidPlant RootsPlants, Genetically ModifiedPlants, MedicinalRegenerationSpectrophotometry, Ultraviolet

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19521847

Citation

Subotić, Angelina, et al. "Spontaneous Plant Regeneration and Production of Secondary Metabolites From Hairy Root Cultures of Centaurium Erythraea Rafn." Methods in Molecular Biology (Clifton, N.J.), vol. 547, 2009, pp. 205-15.
Subotić A, Jevremović S, Grubisić D, et al. Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn. Methods Mol Biol. 2009;547:205-15.
Subotić, A., Jevremović, S., Grubisić, D., & Janković, T. (2009). Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn. Methods in Molecular Biology (Clifton, N.J.), 547, 205-15. https://doi.org/10.1007/978-1-60327-287-2_17
Subotić A, et al. Spontaneous Plant Regeneration and Production of Secondary Metabolites From Hairy Root Cultures of Centaurium Erythraea Rafn. Methods Mol Biol. 2009;547:205-15. PubMed PMID: 19521847.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Spontaneous plant regeneration and production of secondary metabolites from hairy root cultures of Centaurium erythraea Rafn. AU - Subotić,Angelina, AU - Jevremović,Sladana, AU - Grubisić,Dragoljub, AU - Janković,Teodora, PY - 2009/6/13/entrez PY - 2009/6/13/pubmed PY - 2009/7/15/medline SP - 205 EP - 15 JF - Methods in molecular biology (Clifton, N.J.) JO - Methods Mol Biol VL - 547 N2 - We have established an efficient protocol for plant regeneration and production of secondary metabolites in hairy root culture of Centaurium erythraea Rafn. Because the hairy roots and regenerated plants produce bitter secoiridoid glucosides and xanthones similar to the plants in nature, the use of in vitro cultures as an alternative source of their production is feasible. This chapter describes a protocol for the induction of adventitious shoots and transgenic plants from hairy root cultures of C. erythraea and their phytochemical analysis. SN - 1064-3745 UR - https://www.unboundmedicine.com/medline/citation/19521847/Spontaneous_plant_regeneration_and_production_of_secondary_metabolites_from_hairy_root_cultures_of_Centaurium_erythraea_Rafn_ L2 - https://dx.doi.org/10.1007/978-1-60327-287-2_17 DB - PRIME DP - Unbound Medicine ER -