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Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide.
Anal Chem. 2009 Jul 15; 81(14):5603-13.AC

Abstract

In this paper, the characterization and application of a chemically reduced graphene oxide modified glassy carbon (CR-GO/GC) electrode, a novel electrode system, for the preparation of electrochemical sensing and biosensing platform are proposed. Different kinds of important inorganic and organic electroactive compounds (i.e., probe molecule (potassium ferricyanide), free bases of DNA (guanine (G), adenine (A), thymine (T), and cytosine (C)), oxidase/dehydrogenase-related molecules (hydrogen peroxide (H2O2)/beta-nicotinamide adenine dinucleotide (NADH)), neurotransmitters (dopamine (DA)), and other biological molecules (ascorbic acid (AA), uric acid (UA), and acetaminophen (APAP)) were employed to study their electrochemical responses at the CR-GO/GC electrode, which shows more favorable electron transfer kinetics than graphite modified glassy carbon (graphite/GC) and glassy carbon (GC) electrodes. The greatly enhanced electrochemical reactivity of the four free bases of DNA at the CR-GO/GC electrode compared with that at graphite/GC and GC electrodes makes the CR-GO/GC electrode a better choice for the electrochemical biosensing of four DNA bases in both the single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) at physiological pH without a prehydrolysis step. This allows us to detect a single-nucleotide polymorphism (SNP) site for short oligomers with a particular sequence at the CR-GO/GC electrode without any hybridization or labeling processes in this work, suggesting the potential applications of CR-GO in the label-free electrochemical detection of DNA hybridization or DNA damage for further research. Based on the greatly enhanced electrochemical reactivity of H2O2 and NADH at the CR-GO/GC electrode, CR-GO/GC electrode-based bioelectrodes (in connection with glucose oxidase (GOD) and alcohol dehydrogenase (ADH)) show a better analytical performance for the detection of glucose and ethanol compared with graphite/GC- or GC-based bioelectrodes. By comparing the electrochemical performance of CR-GO with that of the conventional graphite and GC, we reveal that CR-GO with the nature of a single sheet showing favorable electrochemical activity should be a kind of more robust and advanced carbon electrode material which may hold great promise for electrochemical sensors and biosensors design.

Links

Publisher Full Text (DOI)

Authors+Show Affiliations

Zhou M
State Key Laboratory of Electroanalytical Chemistry, Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, People's Republic of China.
Zhai Y
No affiliation info available
Dong S
No affiliation info available

MeSH

Biosensing TechniquesCatalysisChemistry Techniques, AnalyticalDNA, Single-StrandedElectrochemistryElectrodesGraphiteHydrogen PeroxideNADOxidation-ReductionPolymorphism, Single Nucleotide

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19522529

Citation

Zhou, Ming, et al. "Electrochemical Sensing and Biosensing Platform Based On Chemically Reduced Graphene Oxide." Analytical Chemistry, vol. 81, no. 14, 2009, pp. 5603-13.
Zhou M, Zhai Y, Dong S. Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. Anal Chem. 2009;81(14):5603-13.
Zhou, M., Zhai, Y., & Dong, S. (2009). Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. Analytical Chemistry, 81(14), 5603-13. https://doi.org/10.1021/ac900136z
Zhou M, Zhai Y, Dong S. Electrochemical Sensing and Biosensing Platform Based On Chemically Reduced Graphene Oxide. Anal Chem. 2009 Jul 15;81(14):5603-13. PubMed PMID: 19522529.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Electrochemical sensing and biosensing platform based on chemically reduced graphene oxide. AU - Zhou,Ming, AU - Zhai,Yueming, AU - Dong,Shaojun, PY - 2009/6/16/entrez PY - 2009/6/16/pubmed PY - 2009/10/7/medline SP - 5603 EP - 13 JF - Analytical chemistry JO - Anal Chem VL - 81 IS - 14 N2 - In this paper, the characterization and application of a chemically reduced graphene oxide modified glassy carbon (CR-GO/GC) electrode, a novel electrode system, for the preparation of electrochemical sensing and biosensing platform are proposed. Different kinds of important inorganic and organic electroactive compounds (i.e., probe molecule (potassium ferricyanide), free bases of DNA (guanine (G), adenine (A), thymine (T), and cytosine (C)), oxidase/dehydrogenase-related molecules (hydrogen peroxide (H2O2)/beta-nicotinamide adenine dinucleotide (NADH)), neurotransmitters (dopamine (DA)), and other biological molecules (ascorbic acid (AA), uric acid (UA), and acetaminophen (APAP)) were employed to study their electrochemical responses at the CR-GO/GC electrode, which shows more favorable electron transfer kinetics than graphite modified glassy carbon (graphite/GC) and glassy carbon (GC) electrodes. The greatly enhanced electrochemical reactivity of the four free bases of DNA at the CR-GO/GC electrode compared with that at graphite/GC and GC electrodes makes the CR-GO/GC electrode a better choice for the electrochemical biosensing of four DNA bases in both the single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) at physiological pH without a prehydrolysis step. This allows us to detect a single-nucleotide polymorphism (SNP) site for short oligomers with a particular sequence at the CR-GO/GC electrode without any hybridization or labeling processes in this work, suggesting the potential applications of CR-GO in the label-free electrochemical detection of DNA hybridization or DNA damage for further research. Based on the greatly enhanced electrochemical reactivity of H2O2 and NADH at the CR-GO/GC electrode, CR-GO/GC electrode-based bioelectrodes (in connection with glucose oxidase (GOD) and alcohol dehydrogenase (ADH)) show a better analytical performance for the detection of glucose and ethanol compared with graphite/GC- or GC-based bioelectrodes. By comparing the electrochemical performance of CR-GO with that of the conventional graphite and GC, we reveal that CR-GO with the nature of a single sheet showing favorable electrochemical activity should be a kind of more robust and advanced carbon electrode material which may hold great promise for electrochemical sensors and biosensors design. SN - 1520-6882 UR - https://www.unboundmedicine.com/medline/citation/19522529/Electrochemical_sensing_and_biosensing_platform_based_on_chemically_reduced_graphene_oxide_ L2 - https://doi.org/10.1021/ac900136z DB - PRIME DP - Unbound Medicine ER -