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Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq.

Abstract

BACKGROUND

Mentha longifolia L. (Lamiaceae) leaves have been traditionally implemented in the treatment of minor sore throat and minor mouth or throat irritation by the indigenous people of Iraq, although the compounds responsible for the medicinal properties have not been identified. In the present study, an antimicrobial compound was isolated and characterized, and its biological activity was assessed.

METHODS

The compound was isolated and characterized from the extracted essential oil using different spectral techniques: TLC, FTIR spectra and HPLC. Antimicrobial activity of the compound was assessed using both disc diffusion and microdilution method in 96 multi-well microtiter plates.

RESULTS

A known compound was isolated from the essential oil of the plant and was identified as (-) menthol. The isolated compound was investigated for its antimicrobial activity against seven selected pathogenic and non-pathogenic microorganisms: Staphylococcus aureus, Streptococcus mutans, Streptococcus faecalis, Streptococcus pyogenis, Lactobacillus acidophilus, Pseudomonas aeruginosa and the yeast Candida albicans. Menthol at different concentrations (1:1, 1:5, 1:10, 1:20) was active against all tested bacteria except for P. aeruginosa, and the highest inhibitory effect was observed against S. mutans (zone of inhibition: 25.3 mm) using the disc diffusion method. Minimal inhibitory concentration MIC values ranged from 15.6-125.0 microg/ml, and the most promising results were observed against S. aureus and S. mutans (MIC 15.6 microg/ml) while, S. faecalis, S. pyogenis and L. acidophilus ranked next (MIC 31.2 microg/ml). Furthermore, menthol achieved considerable antifungal activity against the yeast C. albicans (zone of inhibition range: 7.1-18.5 mm; MIC: 125.0).

CONCLUSION

The isolation of an antimicrobial compound from M. longifolia leaves validates the use of this plant in the treatment of minor sore throat and minor mouth or throat irritation.

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  • Authors+Show Affiliations

    Department of Biology, College of Education, University of Mosul, Mosul, Iraq. firas.bayati@gmail.com

    Source

    MeSH

    Anti-Infective Agents
    Candida albicans
    Chromatography, High Pressure Liquid
    Chromatography, Thin Layer
    Gram-Negative Bacteria
    Gram-Positive Bacteria
    Iraq
    Mentha
    Menthol
    Microbial Sensitivity Tests
    Plant Extracts
    Plant Leaves
    Spectroscopy, Fourier Transform Infrared

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19523224

    Citation

    Al-Bayati, Firas A.. "Isolation and Identification of Antimicrobial Compound From Mentha Longifolia L. Leaves Grown Wild in Iraq." Annals of Clinical Microbiology and Antimicrobials, vol. 8, 2009, p. 20.
    Al-Bayati FA. Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq. Ann Clin Microbiol Antimicrob. 2009;8:20.
    Al-Bayati, F. A. (2009). Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq. Annals of Clinical Microbiology and Antimicrobials, 8, p. 20. doi:10.1186/1476-0711-8-20.
    Al-Bayati FA. Isolation and Identification of Antimicrobial Compound From Mentha Longifolia L. Leaves Grown Wild in Iraq. Ann Clin Microbiol Antimicrob. 2009 Jun 12;8:20. PubMed PMID: 19523224.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Isolation and identification of antimicrobial compound from Mentha longifolia L. leaves grown wild in Iraq. A1 - Al-Bayati,Firas A, Y1 - 2009/06/12/ PY - 2009/04/01/received PY - 2009/06/12/accepted PY - 2009/6/16/entrez PY - 2009/6/16/pubmed PY - 2009/8/27/medline SP - 20 EP - 20 JF - Annals of clinical microbiology and antimicrobials JO - Ann. Clin. Microbiol. Antimicrob. VL - 8 N2 - BACKGROUND: Mentha longifolia L. (Lamiaceae) leaves have been traditionally implemented in the treatment of minor sore throat and minor mouth or throat irritation by the indigenous people of Iraq, although the compounds responsible for the medicinal properties have not been identified. In the present study, an antimicrobial compound was isolated and characterized, and its biological activity was assessed. METHODS: The compound was isolated and characterized from the extracted essential oil using different spectral techniques: TLC, FTIR spectra and HPLC. Antimicrobial activity of the compound was assessed using both disc diffusion and microdilution method in 96 multi-well microtiter plates. RESULTS: A known compound was isolated from the essential oil of the plant and was identified as (-) menthol. The isolated compound was investigated for its antimicrobial activity against seven selected pathogenic and non-pathogenic microorganisms: Staphylococcus aureus, Streptococcus mutans, Streptococcus faecalis, Streptococcus pyogenis, Lactobacillus acidophilus, Pseudomonas aeruginosa and the yeast Candida albicans. Menthol at different concentrations (1:1, 1:5, 1:10, 1:20) was active against all tested bacteria except for P. aeruginosa, and the highest inhibitory effect was observed against S. mutans (zone of inhibition: 25.3 mm) using the disc diffusion method. Minimal inhibitory concentration MIC values ranged from 15.6-125.0 microg/ml, and the most promising results were observed against S. aureus and S. mutans (MIC 15.6 microg/ml) while, S. faecalis, S. pyogenis and L. acidophilus ranked next (MIC 31.2 microg/ml). Furthermore, menthol achieved considerable antifungal activity against the yeast C. albicans (zone of inhibition range: 7.1-18.5 mm; MIC: 125.0). CONCLUSION: The isolation of an antimicrobial compound from M. longifolia leaves validates the use of this plant in the treatment of minor sore throat and minor mouth or throat irritation. SN - 1476-0711 UR - https://www.unboundmedicine.com/medline/citation/19523224/Isolation_and_identification_of_antimicrobial_compound_from_Mentha_longifolia_L__leaves_grown_wild_in_Iraq_ L2 - https://ann-clinmicrob.biomedcentral.com/articles/10.1186/1476-0711-8-20 DB - PRIME DP - Unbound Medicine ER -