Tags

Type your tag names separated by a space and hit enter

Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test.
Mutat Res. 2009 Nov-Dec; 680(1-2):2-6.MR

Abstract

In the present study, the genotoxicity of chlorpyrifos was evaluated in the Chinese toad by using Comet assay and Micronucleus test (MN), as the potential tools for the assessment of genotoxicity. The first step was determined by the acute toxicity of chlorpyrifos. Tadpoles were exposed to the series of relatively high concentrations of chlorpyrifos for 96 h. LC(50) values at 24, 48, 72, and 96 h were 3.63, 1.17, 0.82, and 0.80 mgl(-1), respectively. Secondly, the Micronucleus test was used for detecting chromosome damage in Chinese toad tadpoles exposed to the sublethal concentrations of chlorpyrifos and methyl methane sulfonate (MMS), which indicated that they induced chromosomal lesion in erythrocytes of Bufo bufo gargarizans tadpoles. Thirdly, the significant (P < 0.05 concentration-dependent increase in DNA damage (as indicated by Tail DNA%, Tail length, Olive tail moment)) were observed in erythrocytes and liver cells of tadpoles exposed to the sublethal concentrations of chlorpyrifos and MMS by Comet assay. To our knowledge, this is the first report to describe the use of B. bufo gargarizans for genotoxicity assessment of chlorpyrifos.

Authors+Show Affiliations

School of Agriculture and Food Science, Zhejiang Forestry University, Lin'an, HangZhou 311300, China.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19524702

Citation

Yin, XiaoHui, et al. "Genotoxicity Evaluation of Chlorpyrifos to Amphibian Chinese Toad (Amphibian: Anura) By Comet Assay and Micronucleus Test." Mutation Research, vol. 680, no. 1-2, 2009, pp. 2-6.
Yin X, Zhu G, Li XB, et al. Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. Mutat Res. 2009;680(1-2):2-6.
Yin, X., Zhu, G., Li, X. B., & Liu, S. (2009). Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. Mutation Research, 680(1-2), 2-6. https://doi.org/10.1016/j.mrgentox.2009.05.018
Yin X, et al. Genotoxicity Evaluation of Chlorpyrifos to Amphibian Chinese Toad (Amphibian: Anura) By Comet Assay and Micronucleus Test. Mutat Res. 2009 Nov-Dec;680(1-2):2-6. PubMed PMID: 19524702.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Genotoxicity evaluation of chlorpyrifos to amphibian Chinese toad (Amphibian: Anura) by Comet assay and Micronucleus test. AU - Yin,XiaoHui, AU - Zhu,GuoNian, AU - Li,Xian Bing, AU - Liu,ShaoYing, Y1 - 2009/06/12/ PY - 2008/08/01/received PY - 2009/05/05/revised PY - 2009/05/24/accepted PY - 2009/6/16/entrez PY - 2009/6/16/pubmed PY - 2010/4/7/medline SP - 2 EP - 6 JF - Mutation research JO - Mutat Res VL - 680 IS - 1-2 N2 - In the present study, the genotoxicity of chlorpyrifos was evaluated in the Chinese toad by using Comet assay and Micronucleus test (MN), as the potential tools for the assessment of genotoxicity. The first step was determined by the acute toxicity of chlorpyrifos. Tadpoles were exposed to the series of relatively high concentrations of chlorpyrifos for 96 h. LC(50) values at 24, 48, 72, and 96 h were 3.63, 1.17, 0.82, and 0.80 mgl(-1), respectively. Secondly, the Micronucleus test was used for detecting chromosome damage in Chinese toad tadpoles exposed to the sublethal concentrations of chlorpyrifos and methyl methane sulfonate (MMS), which indicated that they induced chromosomal lesion in erythrocytes of Bufo bufo gargarizans tadpoles. Thirdly, the significant (P < 0.05 concentration-dependent increase in DNA damage (as indicated by Tail DNA%, Tail length, Olive tail moment)) were observed in erythrocytes and liver cells of tadpoles exposed to the sublethal concentrations of chlorpyrifos and MMS by Comet assay. To our knowledge, this is the first report to describe the use of B. bufo gargarizans for genotoxicity assessment of chlorpyrifos. SN - 0027-5107 UR - https://www.unboundmedicine.com/medline/citation/19524702/Genotoxicity_evaluation_of_chlorpyrifos_to_amphibian_Chinese_toad__Amphibian:_Anura__by_Comet_assay_and_Micronucleus_test_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5718(09)00193-4 DB - PRIME DP - Unbound Medicine ER -