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Hydroxyethyl starch interferes with human blood ex vivo coagulation, platelet function and sedimentation.
Acta Anaesthesiol Taiwan. 2009 Jun; 47(2):71-8.AA

Abstract

BACKGROUND

Hydroxyethyl starch (HES) solutions are widely used for intravascular volume expansion. In Taiwan, the medium molecular weight of HES 200/0.5 and HES 130/0.4 solutions are most commonly used. It has been demonstrated that HES may affect coagulation and platelet function significantly. However, the differential effects of each medium molecular weight HES on platelets remain poorly reported. Therefore, we studied the influence of the two HES solutions on platelet function in vitro by mixing whole blood with different proportions of HES 130 kD, HES 200 kD, and saline to determine the differences.

METHODS

Human blood samples for platelet function analyzer (PFA), aggregometry and blood/HES mixed test were drawn from the antecubital vein and put into test tubes containing 3.2% trisodium citrate (blood:citrate, 9:1). The specimens were divided into four groups, designated as whole blood, 10%, 20%, and 30% dilution with normal saline (N/S), HES130 or HES200 solution. The platelet function of each sample was measured by both PFA and platelet aggregometry.

RESULTS

The results showed that the PFA-100 closure times CEPI-CT and CADP-CT were significantly prolonged in the samples diluted with normal saline, HES130 and HES200 than in the controls. The ADP triggered whole blood aggregometry showed that attenuated impedance was observed in samples of 20% diluted with HES130 and HES200 groups. The blood/HES mixed sedimentation test showed significantly increased proportion of the upper liquid layer in the HES200 group than in other groups.

CONCLUSION

Our data demonstrated that HES200 and HES130 possess noticeably inhibitory effects on platelet function, especially when the HES replaced proportion was more than 20%. HES200 has a greater effect on blood cells and plasma separation than does HES130.

Authors+Show Affiliations

Department of Anesthesiology, Chang Gung Memorial Hospital, Linkou, Taiwan, R.O.C.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19527967

Citation

Liu, Fu-Chao, et al. "Hydroxyethyl Starch Interferes With Human Blood Ex Vivo Coagulation, Platelet Function and Sedimentation." Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists, vol. 47, no. 2, 2009, pp. 71-8.
Liu FC, Liao CH, Chang YW, et al. Hydroxyethyl starch interferes with human blood ex vivo coagulation, platelet function and sedimentation. Acta Anaesthesiol Taiwan. 2009;47(2):71-8.
Liu, F. C., Liao, C. H., Chang, Y. W., Liou, J. T., & Day, Y. J. (2009). Hydroxyethyl starch interferes with human blood ex vivo coagulation, platelet function and sedimentation. Acta Anaesthesiologica Taiwanica : Official Journal of the Taiwan Society of Anesthesiologists, 47(2), 71-8. https://doi.org/10.1016/S1875-4597(09)60027-8
Liu FC, et al. Hydroxyethyl Starch Interferes With Human Blood Ex Vivo Coagulation, Platelet Function and Sedimentation. Acta Anaesthesiol Taiwan. 2009;47(2):71-8. PubMed PMID: 19527967.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hydroxyethyl starch interferes with human blood ex vivo coagulation, platelet function and sedimentation. AU - Liu,Fu-Chao, AU - Liao,Chang-Hui, AU - Chang,Yao-Wen, AU - Liou,Jiin-Tarng, AU - Day,Yuan-Ji, PY - 2009/6/17/entrez PY - 2009/6/17/pubmed PY - 2009/8/26/medline SP - 71 EP - 8 JF - Acta anaesthesiologica Taiwanica : official journal of the Taiwan Society of Anesthesiologists JO - Acta Anaesthesiol Taiwan VL - 47 IS - 2 N2 - BACKGROUND: Hydroxyethyl starch (HES) solutions are widely used for intravascular volume expansion. In Taiwan, the medium molecular weight of HES 200/0.5 and HES 130/0.4 solutions are most commonly used. It has been demonstrated that HES may affect coagulation and platelet function significantly. However, the differential effects of each medium molecular weight HES on platelets remain poorly reported. Therefore, we studied the influence of the two HES solutions on platelet function in vitro by mixing whole blood with different proportions of HES 130 kD, HES 200 kD, and saline to determine the differences. METHODS: Human blood samples for platelet function analyzer (PFA), aggregometry and blood/HES mixed test were drawn from the antecubital vein and put into test tubes containing 3.2% trisodium citrate (blood:citrate, 9:1). The specimens were divided into four groups, designated as whole blood, 10%, 20%, and 30% dilution with normal saline (N/S), HES130 or HES200 solution. The platelet function of each sample was measured by both PFA and platelet aggregometry. RESULTS: The results showed that the PFA-100 closure times CEPI-CT and CADP-CT were significantly prolonged in the samples diluted with normal saline, HES130 and HES200 than in the controls. The ADP triggered whole blood aggregometry showed that attenuated impedance was observed in samples of 20% diluted with HES130 and HES200 groups. The blood/HES mixed sedimentation test showed significantly increased proportion of the upper liquid layer in the HES200 group than in other groups. CONCLUSION: Our data demonstrated that HES200 and HES130 possess noticeably inhibitory effects on platelet function, especially when the HES replaced proportion was more than 20%. HES200 has a greater effect on blood cells and plasma separation than does HES130. SN - 1875-4597 UR - https://www.unboundmedicine.com/medline/citation/19527967/Hydroxyethyl_starch_interferes_with_human_blood_ex_vivo_coagulation_platelet_function_and_sedimentation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1875-4597(09)60027-8 DB - PRIME DP - Unbound Medicine ER -