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Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco.
Theor Appl Genet. 2009 Aug; 119(4):695-704.TA

Abstract

Rye is a diploid crop species with many outstanding qualities, and is important as a source of new traits for wheat and triticale improvement. Rye is highly tolerant of aluminum (Al) toxicity, and possesses a complex structure at the Alt4 Al tolerance locus not found at the corresponding locus in wheat. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies, and assess the library's suitability for investigating Al tolerance genes. The library provides 6 x genome coverage of the 8.1 Gb rye genome, has an average insert size of 131 kb, and contains only ~2% of empty or organelle-derived clones. Genetic analysis attributed the Al tolerance of Blanco to the Alt4 locus on the short arm of chromosome 7R, and revealed the presence of multiple allelic variants (haplotypes) of the Alt4 locus in the BAC library. BAC clones containing ALMT1 gene clusters from several Alt4 haplotypes were identified, and will provide useful starting points for exploring the basis for the structural variability and functional specialization of ALMT1 genes at this locus.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Shi BJ
Australian Centre for Plant Functional Genomics (ACPFG), School of Agriculture, Food and Wine, University of Adelaide, PMB1, Glen Osmond, SA, 5064, Australia.
Gustafson JP
No affiliation info available
Button J
No affiliation info available
Miyazaki J
No affiliation info available
Pallotta M
No affiliation info available
Gustafson N
No affiliation info available
Zhou H
No affiliation info available
Langridge P
No affiliation info available
Collins NC
No affiliation info available

MeSH

Adaptation, PhysiologicalAluminumBlotting, SouthernChromosomes, Artificial, BacterialChromosomes, PlantContig MappingDNA ProbesDNA, PlantGenes, PlantGenetic MarkersGenomic LibraryHaplotypesMultigene FamilyPhysical Chromosome MappingSecale

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19529908

Citation

Shi, B-J, et al. "Physical Analysis of the Complex Rye (Secale Cereale L.) Alt4 Aluminium (aluminum) Tolerance Locus Using a Whole-genome BAC Library of Rye Cv. Blanco." TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, vol. 119, no. 4, 2009, pp. 695-704.
Shi BJ, Gustafson JP, Button J, et al. Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco. Theor Appl Genet. 2009;119(4):695-704.
Shi, B. J., Gustafson, J. P., Button, J., Miyazaki, J., Pallotta, M., Gustafson, N., Zhou, H., Langridge, P., & Collins, N. C. (2009). Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco. TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik, 119(4), 695-704. https://doi.org/10.1007/s00122-009-1080-8
Shi BJ, et al. Physical Analysis of the Complex Rye (Secale Cereale L.) Alt4 Aluminium (aluminum) Tolerance Locus Using a Whole-genome BAC Library of Rye Cv. Blanco. Theor Appl Genet. 2009;119(4):695-704. PubMed PMID: 19529908.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Physical analysis of the complex rye (Secale cereale L.) Alt4 aluminium (aluminum) tolerance locus using a whole-genome BAC library of rye cv. Blanco. AU - Shi,B-J, AU - Gustafson,J P, AU - Button,J, AU - Miyazaki,J, AU - Pallotta,M, AU - Gustafson,N, AU - Zhou,H, AU - Langridge,P, AU - Collins,N C, Y1 - 2009/06/16/ PY - 2009/02/09/received PY - 2009/05/21/accepted PY - 2009/6/17/entrez PY - 2009/6/17/pubmed PY - 2009/10/8/medline SP - 695 EP - 704 JF - TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik JO - Theor Appl Genet VL - 119 IS - 4 N2 - Rye is a diploid crop species with many outstanding qualities, and is important as a source of new traits for wheat and triticale improvement. Rye is highly tolerant of aluminum (Al) toxicity, and possesses a complex structure at the Alt4 Al tolerance locus not found at the corresponding locus in wheat. Here we describe a BAC library of rye cv. Blanco, representing a valuable resource for rye molecular genetic studies, and assess the library's suitability for investigating Al tolerance genes. The library provides 6 x genome coverage of the 8.1 Gb rye genome, has an average insert size of 131 kb, and contains only ~2% of empty or organelle-derived clones. Genetic analysis attributed the Al tolerance of Blanco to the Alt4 locus on the short arm of chromosome 7R, and revealed the presence of multiple allelic variants (haplotypes) of the Alt4 locus in the BAC library. BAC clones containing ALMT1 gene clusters from several Alt4 haplotypes were identified, and will provide useful starting points for exploring the basis for the structural variability and functional specialization of ALMT1 genes at this locus. SN - 1432-2242 UR - https://www.unboundmedicine.com/medline/citation/19529908/Physical_analysis_of_the_complex_rye__Secale_cereale_L___Alt4_aluminium__aluminum__tolerance_locus_using_a_whole_genome_BAC_library_of_rye_cv__Blanco_ L2 - https://dx.doi.org/10.1007/s00122-009-1080-8 DB - PRIME DP - Unbound Medicine ER -