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Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins.
J Agric Food Chem. 2009 Jul 22; 57(14):6007-13.JA

Abstract

Peanut skins, a byproduct of the peanut butter industry, are a rich source of proanthocyanidins, which might be used in food supplements. Data on the molecular diversity of proanthocyanidins in peanut skins is limited and conflicting with respect to the ratio of double- (A-type) versus single-linked (B-type) flavan-3-ol units. NP- and RP-HPLC-MS were used as tools to analyze the molecular diversity of proanthocyanidins in a 20% (v/v) methanol extract of peanut skins. NP-HPLC was used to prepurify monomeric to pentameric fractions, which were further separated and characterized by RP-HPLC-MS. With this method, 83 different proanthocyanidin molecular species were characterized and quantified. Furthermore, it was possible to determine that A-type procyanidin oligomers were predominant and represented 95.0% (w/w) of the extract. In addition, the position of the A-linkages in 16 trimers and 27 tetramers could be determined, which in this case appeared to occur at all possible positions. The majority of trimers and tetramers with one or more A-linkage always had an A-linkage at the terminal unit.

Authors+Show Affiliations

Laboratory of Food Chemistry, Wageningen University, Bomenweg 2, 6700 EV Wageningen, The Netherlands.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19537791

Citation

Appeldoorn, Maaike M., et al. "Combined Normal-phase and Reversed-phase Liquid chromatography/ESI-MS as a Tool to Determine the Molecular Diversity of A-type Procyanidins in Peanut Skins." Journal of Agricultural and Food Chemistry, vol. 57, no. 14, 2009, pp. 6007-13.
Appeldoorn MM, Vincken JP, Sanders M, et al. Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins. J Agric Food Chem. 2009;57(14):6007-13.
Appeldoorn, M. M., Vincken, J. P., Sanders, M., Hollman, P. C., & Gruppen, H. (2009). Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins. Journal of Agricultural and Food Chemistry, 57(14), 6007-13. https://doi.org/10.1021/jf9010092
Appeldoorn MM, et al. Combined Normal-phase and Reversed-phase Liquid chromatography/ESI-MS as a Tool to Determine the Molecular Diversity of A-type Procyanidins in Peanut Skins. J Agric Food Chem. 2009 Jul 22;57(14):6007-13. PubMed PMID: 19537791.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined normal-phase and reversed-phase liquid chromatography/ESI-MS as a tool to determine the molecular diversity of A-type procyanidins in peanut skins. AU - Appeldoorn,Maaike M, AU - Vincken,Jean-Paul, AU - Sanders,Mark, AU - Hollman,Peter C H, AU - Gruppen,Harry, PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/11/17/medline SP - 6007 EP - 13 JF - Journal of agricultural and food chemistry JO - J. Agric. Food Chem. VL - 57 IS - 14 N2 - Peanut skins, a byproduct of the peanut butter industry, are a rich source of proanthocyanidins, which might be used in food supplements. Data on the molecular diversity of proanthocyanidins in peanut skins is limited and conflicting with respect to the ratio of double- (A-type) versus single-linked (B-type) flavan-3-ol units. NP- and RP-HPLC-MS were used as tools to analyze the molecular diversity of proanthocyanidins in a 20% (v/v) methanol extract of peanut skins. NP-HPLC was used to prepurify monomeric to pentameric fractions, which were further separated and characterized by RP-HPLC-MS. With this method, 83 different proanthocyanidin molecular species were characterized and quantified. Furthermore, it was possible to determine that A-type procyanidin oligomers were predominant and represented 95.0% (w/w) of the extract. In addition, the position of the A-linkages in 16 trimers and 27 tetramers could be determined, which in this case appeared to occur at all possible positions. The majority of trimers and tetramers with one or more A-linkage always had an A-linkage at the terminal unit. SN - 1520-5118 UR - https://www.unboundmedicine.com/medline/citation/19537791/Combined_normal_phase_and_reversed_phase_liquid_chromatography/ESI_MS_as_a_tool_to_determine_the_molecular_diversity_of_A_type_procyanidins_in_peanut_skins_ L2 - https://dx.doi.org/10.1021/jf9010092 DB - PRIME DP - Unbound Medicine ER -