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Antinociceptive effects of the non-selective cannabinoid receptor agonist CP 55,940 are absent in CB1(-/-) and not CB2(-/-) mice in models of acute and persistent pain.
Neuropharmacology. 2009 Sep; 57(3):235-41.N

Abstract

Previous studies have suggested a role for both CB1 and CB2 cannabinoid receptors in modulation of nociception. To further examine the role of CB1 and CB2 receptors in antinociception, we evaluated the efficacy of the non-selective cannabinoid receptor agonist, CP 55,940, in models of acute, inflammatory, and neuropathic pain in control mice, CB1 receptor knockout mice, and CB2 receptor knockout mice. In control C57BL/6 mice, administration of CP 55,940 (0.03-0.3 mg/kg, i.p.) reversed complete Freund's adjuvant-induced tactile allodynia, reversed tactile allodynia in the spinal nerve ligation model and inhibited the noxious heat-evoked tail withdrawal response. In addition to its antinociceptive effects, CP 55,940 produced an impairment of motor coordination in the rotarod test. The antinociceptive effects produced by CP 55,940 and associated motor deficits were found to be completely abolished in CB1 receptor knockout mice. In contrast, the antinociceptive effects of CP 55,940 in all pain models were fully retained in CB2 receptor knockout mice, along with the associated motor deficits. The results suggest that the antinociceptive effects of CP 55,940 in models of acute and persistent pain, along with the associated motor deficits, are mediated by CB1 receptors, and likely not CB2 receptors.

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Authors+Show Affiliations

Sain NM
Merck Research Laboratories, Department of Pain Research, WP-46-300, West Point, PA 19486, USA. nova_sain@merck.com
Liang A
No affiliation info available
Kane SA
No affiliation info available
Urban MO
No affiliation info available

MeSH

AnalgesicsAnimalsCyclohexanolsDisease Models, AnimalDose-Response Relationship, DrugFreund's AdjuvantHot TemperatureMiceMice, Inbred C57BLMice, KnockoutMotor ActivityPainPain MeasurementPhysical StimulationReceptor, Cannabinoid, CB1Receptor, Cannabinoid, CB2Spinal Nerves

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19538975

Citation

Sain, Nova M H., et al. "Antinociceptive Effects of the Non-selective Cannabinoid Receptor Agonist CP 55,940 Are Absent in CB1(-/-) and Not CB2(-/-) Mice in Models of Acute and Persistent Pain." Neuropharmacology, vol. 57, no. 3, 2009, pp. 235-41.
Sain NM, Liang A, Kane SA, et al. Antinociceptive effects of the non-selective cannabinoid receptor agonist CP 55,940 are absent in CB1(-/-) and not CB2(-/-) mice in models of acute and persistent pain. Neuropharmacology. 2009;57(3):235-41.
Sain, N. M., Liang, A., Kane, S. A., & Urban, M. O. (2009). Antinociceptive effects of the non-selective cannabinoid receptor agonist CP 55,940 are absent in CB1(-/-) and not CB2(-/-) mice in models of acute and persistent pain. Neuropharmacology, 57(3), 235-41. https://doi.org/10.1016/j.neuropharm.2009.06.004
Sain NM, et al. Antinociceptive Effects of the Non-selective Cannabinoid Receptor Agonist CP 55,940 Are Absent in CB1(-/-) and Not CB2(-/-) Mice in Models of Acute and Persistent Pain. Neuropharmacology. 2009;57(3):235-41. PubMed PMID: 19538975.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Antinociceptive effects of the non-selective cannabinoid receptor agonist CP 55,940 are absent in CB1(-/-) and not CB2(-/-) mice in models of acute and persistent pain. AU - Sain,Nova M H, AU - Liang,Annie, AU - Kane,Stefanie A, AU - Urban,Mark O, Y1 - 2009/06/16/ PY - 2008/12/09/received PY - 2009/06/03/revised PY - 2009/06/04/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/12/16/medline SP - 235 EP - 41 JF - Neuropharmacology JO - Neuropharmacology VL - 57 IS - 3 N2 - Previous studies have suggested a role for both CB1 and CB2 cannabinoid receptors in modulation of nociception. To further examine the role of CB1 and CB2 receptors in antinociception, we evaluated the efficacy of the non-selective cannabinoid receptor agonist, CP 55,940, in models of acute, inflammatory, and neuropathic pain in control mice, CB1 receptor knockout mice, and CB2 receptor knockout mice. In control C57BL/6 mice, administration of CP 55,940 (0.03-0.3 mg/kg, i.p.) reversed complete Freund's adjuvant-induced tactile allodynia, reversed tactile allodynia in the spinal nerve ligation model and inhibited the noxious heat-evoked tail withdrawal response. In addition to its antinociceptive effects, CP 55,940 produced an impairment of motor coordination in the rotarod test. The antinociceptive effects produced by CP 55,940 and associated motor deficits were found to be completely abolished in CB1 receptor knockout mice. In contrast, the antinociceptive effects of CP 55,940 in all pain models were fully retained in CB2 receptor knockout mice, along with the associated motor deficits. The results suggest that the antinociceptive effects of CP 55,940 in models of acute and persistent pain, along with the associated motor deficits, are mediated by CB1 receptors, and likely not CB2 receptors. SN - 1873-7064 UR - https://www.unboundmedicine.com/medline/citation/19538975/Antinociceptive_effects_of_the_non_selective_cannabinoid_receptor_agonist_CP_55940_are_absent_in_CB1__/___and_not_CB2__/___mice_in_models_of_acute_and_persistent_pain_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0028-3908(09)00158-0 DB - PRIME DP - Unbound Medicine ER -