Tags

Type your tag names separated by a space and hit enter

Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease.
Nutrition. 2009 Jul-Aug; 25(7-8):762-8.N

Abstract

OBJECTIVE

The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation.

METHODS

Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells.

RESULTS

Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-alpha and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination.

CONCLUSION

Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation.

Authors+Show Affiliations

Third Department of Medicine, Charles University and General University Hospital in Prague, Czech Republic.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19539174

Citation

Roubicek, Tomas, et al. "Increased Production of Proinflammatory Cytokines in Adipose Tissue of Patients With End-stage Renal Disease." Nutrition (Burbank, Los Angeles County, Calif.), vol. 25, no. 7-8, 2009, pp. 762-8.
Roubicek T, Bartlova M, Krajickova J, et al. Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease. Nutrition. 2009;25(7-8):762-8.
Roubicek, T., Bartlova, M., Krajickova, J., Haluzikova, D., Mraz, M., Lacinova, Z., Kudla, M., Teplan, V., & Haluzik, M. (2009). Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease. Nutrition (Burbank, Los Angeles County, Calif.), 25(7-8), 762-8. https://doi.org/10.1016/j.nut.2008.12.012
Roubicek T, et al. Increased Production of Proinflammatory Cytokines in Adipose Tissue of Patients With End-stage Renal Disease. Nutrition. 2009;25(7-8):762-8. PubMed PMID: 19539174.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Increased production of proinflammatory cytokines in adipose tissue of patients with end-stage renal disease. AU - Roubicek,Tomas, AU - Bartlova,Marketa, AU - Krajickova,Jana, AU - Haluzikova,Denisa, AU - Mraz,Milos, AU - Lacinova,Zdena, AU - Kudla,Michal, AU - Teplan,Vladimir, AU - Haluzik,Martin, PY - 2008/06/24/received PY - 2008/10/30/revised PY - 2008/12/18/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/10/21/medline SP - 762 EP - 8 JF - Nutrition (Burbank, Los Angeles County, Calif.) JO - Nutrition VL - 25 IS - 7-8 N2 - OBJECTIVE: The number of patients with end-stage renal disease (ESRD) is rising and these patients are at higher risk of cardiovascular disease. We studied the role of hormonal production of adipose tissue in the development of chronic inflammation in patients with ESRD before kidney transplantation. METHODS: Fifteen women with ESRD and 17 healthy women (control) underwent single blood drawing and visceral and subcutaneous adipose tissue sampling during surgery (kidney transplantation in the ESRD group or cholecystectomy in the control group). Serum concentrations of C-reactive protein, interleukin-6, tumor necrosis factor-alpha, leptin, adiponectin, resistin, monocyte chemoattractant protein-1 were measured. Messenger RNA expression of the same hormones, adiponectin receptors 1 and 2 and immunocompetent cell marker CD68 in subcutaneous and visceral samples were measured using real-time polymerase chain reaction. Adipose tissue was examined immunohistochemically for CD68-positive cells. RESULTS: Serum concentrations of C-reactive protein, adiponectin, resistin, interleukin-6, tumor necrosis factor-alpha, and monocyte chemoattractant protein-1 were significantly higher in the ESRD versus control group. Subcutaneous and visceral mRNA expressions of tumor necrosis factor-alpha and CD68 were significantly increased in the ESRD versus control group. Adiponectin receptor-1 and monocyte chemoattractant protein-1 mRNA expressions were significantly higher in visceral but not in subcutaneous adipose tissue of the ESRD group. Messenger RNA expressions of resistin, leptin, adiponectin, interleukin-6, and adiponectin receptor-2 in both fat depots did not significantly differ between groups. Increased infiltration of subcutaneous and visceral adipose tissue with CD68-positive immunocompetent cells was found in the ESRD group by histologic examination. CONCLUSION: Subcutaneous and visceral adipose tissues in ESRD express higher amounts of proinflammatory cytokines and may play a role in the development of systemic inflammation. SN - 1873-1244 UR - https://www.unboundmedicine.com/medline/citation/19539174/Increased_production_of_proinflammatory_cytokines_in_adipose_tissue_of_patients_with_end_stage_renal_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0899-9007(09)00115-4 DB - PRIME DP - Unbound Medicine ER -