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Identification and characterization of Foxp3(+) gammadelta T cells in mouse and human.
Immunol Lett. 2009 Aug 15; 125(2):105-13.IL

Abstract

Regulatory T cells (Tregs) expressing TCRalphabeta play a critical role in the maintenance of the immune system homeostasis. Tregs express the cell surface markers CD4 and CD25 as well as the transcription factor Foxp3. Foxp3(+)CD4(+)CD25(+)TCRalphabeta(+) Tregs can be generated from mouse and human CD4(+)CD25(-) T cells in vitro via TGF-beta induction. As growing evidences suggest that gammadelta T cells also have immunoregulatory function, we have attempted to identify and characterize Foxp3(+) cells in mouse and human gammadelta T cells. We found that freshly isolated mouse splenic gammadelta T cells did not express Foxp3. When mouse splenocytes were stimulated with anti-TCRgammadelta in the presence of TGF-beta, a population of Foxp3(+) gammadelta T cells appeared, in most of which expressed CD25 as well. Compared with CD25(-) gammadelta T cells, TGF-beta induced CD25(+) gammadelta T cells not only expressed Foxp3, but also had increased TGF-beta and GITR expression. Furthermore, the TGF-beta induced gammadelta T cells mediated a potent immunosuppressive effect on anti-CD3 stimulated T cell activation and proliferation. In contrast, although a small fraction of human peripheral blood and tumor infiltrating gammadelta T cells expressed Foxp3, similar culture condition with anti-TCRgammadelta plus TGF-beta failed to generate functional human Foxp3(+) gammadelta T cells. In conclusion, our results suggest that mouse splenic Foxp3(+) gammadelta T cells with suppressive function can be induced by TCR and TGF-beta costimulation, whereas functional human Foxp3(+) gammadelta T cells in peripheral blood could not be generated under the same condition.

Authors+Show Affiliations

Department of Immunology, School of Basic Medicine, Peking Union Medical College, and Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences, 5 Dong Dan San Tiao, Beijing 100005, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19539651

Citation

Kang, Ning, et al. "Identification and Characterization of Foxp3(+) Gammadelta T Cells in Mouse and Human." Immunology Letters, vol. 125, no. 2, 2009, pp. 105-13.
Kang N, Tang L, Li X, et al. Identification and characterization of Foxp3(+) gammadelta T cells in mouse and human. Immunol Lett. 2009;125(2):105-13.
Kang, N., Tang, L., Li, X., Wu, D., Li, W., Chen, X., Cui, L., Ba, D., & He, W. (2009). Identification and characterization of Foxp3(+) gammadelta T cells in mouse and human. Immunology Letters, 125(2), 105-13. https://doi.org/10.1016/j.imlet.2009.06.005
Kang N, et al. Identification and Characterization of Foxp3(+) Gammadelta T Cells in Mouse and Human. Immunol Lett. 2009 Aug 15;125(2):105-13. PubMed PMID: 19539651.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Identification and characterization of Foxp3(+) gammadelta T cells in mouse and human. AU - Kang,Ning, AU - Tang,Long, AU - Li,Xiaoyan, AU - Wu,Dan, AU - Li,Wenjing, AU - Chen,Xingming, AU - Cui,Lianxian, AU - Ba,Denian, AU - He,Wei, Y1 - 2009/06/17/ PY - 2009/03/19/received PY - 2009/05/25/revised PY - 2009/06/08/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/12/18/medline SP - 105 EP - 13 JF - Immunology letters JO - Immunol. Lett. VL - 125 IS - 2 N2 - Regulatory T cells (Tregs) expressing TCRalphabeta play a critical role in the maintenance of the immune system homeostasis. Tregs express the cell surface markers CD4 and CD25 as well as the transcription factor Foxp3. Foxp3(+)CD4(+)CD25(+)TCRalphabeta(+) Tregs can be generated from mouse and human CD4(+)CD25(-) T cells in vitro via TGF-beta induction. As growing evidences suggest that gammadelta T cells also have immunoregulatory function, we have attempted to identify and characterize Foxp3(+) cells in mouse and human gammadelta T cells. We found that freshly isolated mouse splenic gammadelta T cells did not express Foxp3. When mouse splenocytes were stimulated with anti-TCRgammadelta in the presence of TGF-beta, a population of Foxp3(+) gammadelta T cells appeared, in most of which expressed CD25 as well. Compared with CD25(-) gammadelta T cells, TGF-beta induced CD25(+) gammadelta T cells not only expressed Foxp3, but also had increased TGF-beta and GITR expression. Furthermore, the TGF-beta induced gammadelta T cells mediated a potent immunosuppressive effect on anti-CD3 stimulated T cell activation and proliferation. In contrast, although a small fraction of human peripheral blood and tumor infiltrating gammadelta T cells expressed Foxp3, similar culture condition with anti-TCRgammadelta plus TGF-beta failed to generate functional human Foxp3(+) gammadelta T cells. In conclusion, our results suggest that mouse splenic Foxp3(+) gammadelta T cells with suppressive function can be induced by TCR and TGF-beta costimulation, whereas functional human Foxp3(+) gammadelta T cells in peripheral blood could not be generated under the same condition. SN - 1879-0542 UR - https://www.unboundmedicine.com/medline/citation/19539651/Identification_and_characterization_of_Foxp3_+__gammadelta_T_cells_in_mouse_and_human_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0165-2478(09)00160-6 DB - PRIME DP - Unbound Medicine ER -