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Tetramethylpyrazine suppresses interleukin-8 expression in LPS-stimulated human umbilical vein endothelial cell by blocking ERK, p38 and nulear factor-kappaB signaling pathways.
J Ethnopharmacol. 2009 Aug 17; 125(1):83-9.JE

Abstract

AIM OF THE STUDY

To determine the anti-inflammatory effects of Tetramethylpyrazine (TMP) and to investigate the inhibitory effect of TMP on IL-8 production in human umbilical vein endothelial cells (HUVECs) induced by LPS might be mediated by inhibiting p38, ERK and NF-kappaB signaling pathways.

MATERIALS AND METHODS

HUVECs were treated with or without TMP for 24h before exposure to LPS for 4h. IL-8 gene and protein expressions were determined by RT-PCR and ELISA. Cell viability was determined by methyl thiazoyltetrazolium (MTT) assay. Phosphorylation of ERK1/2 and p38 were examined by western blotting.

RESULTS

TMP inhibits LPS-induced IL-8 production in HUVECs at both the protein and mRNA levels, suggesting that TMP has an antiinflammatory effect on endothelial cells. TMP also inhibited U937 monocyte adhesion to HUVECs stimulated by LPS. LPS-induced phosphorylation of ERK1/2 and p38 were inhibited by TMP. The inhibitory effect of TMP on NF-kappaB (p65) activity was mediated by blocking the consequent translocation of p65 into the nucleus.

CONCLUSIONS

The inhibitory effect of TMP on the LPS-induced IL-8 production is mediated by the NF-kappaB-dependent pathway, and TMP also separately affects the ERK and p38 MAPK pathway. TMP may be beneficial in the treatment of cardiovascular disorders such as atherosclerosis.

Authors+Show Affiliations

Institute of Life Sciences, Chongqing Medical University, Chongqing 400016, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19540326

Citation

Li, Xiu-Ying, et al. "Tetramethylpyrazine Suppresses Interleukin-8 Expression in LPS-stimulated Human Umbilical Vein Endothelial Cell By Blocking ERK, P38 and Nulear factor-kappaB Signaling Pathways." Journal of Ethnopharmacology, vol. 125, no. 1, 2009, pp. 83-9.
Li XY, He JL, Liu HT, et al. Tetramethylpyrazine suppresses interleukin-8 expression in LPS-stimulated human umbilical vein endothelial cell by blocking ERK, p38 and nulear factor-kappaB signaling pathways. J Ethnopharmacol. 2009;125(1):83-9.
Li, X. Y., He, J. L., Liu, H. T., Li, W. M., & Yu, C. (2009). Tetramethylpyrazine suppresses interleukin-8 expression in LPS-stimulated human umbilical vein endothelial cell by blocking ERK, p38 and nulear factor-kappaB signaling pathways. Journal of Ethnopharmacology, 125(1), 83-9. https://doi.org/10.1016/j.jep.2009.06.008
Li XY, et al. Tetramethylpyrazine Suppresses Interleukin-8 Expression in LPS-stimulated Human Umbilical Vein Endothelial Cell By Blocking ERK, P38 and Nulear factor-kappaB Signaling Pathways. J Ethnopharmacol. 2009 Aug 17;125(1):83-9. PubMed PMID: 19540326.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Tetramethylpyrazine suppresses interleukin-8 expression in LPS-stimulated human umbilical vein endothelial cell by blocking ERK, p38 and nulear factor-kappaB signaling pathways. AU - Li,Xiu-Ying, AU - He,Jun-Lin, AU - Liu,Hong-Tao, AU - Li,Wen-Ming, AU - Yu,Chao, Y1 - 2009/06/18/ PY - 2009/04/17/received PY - 2009/06/01/revised PY - 2009/06/10/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/11/3/medline SP - 83 EP - 9 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 125 IS - 1 N2 - AIM OF THE STUDY: To determine the anti-inflammatory effects of Tetramethylpyrazine (TMP) and to investigate the inhibitory effect of TMP on IL-8 production in human umbilical vein endothelial cells (HUVECs) induced by LPS might be mediated by inhibiting p38, ERK and NF-kappaB signaling pathways. MATERIALS AND METHODS: HUVECs were treated with or without TMP for 24h before exposure to LPS for 4h. IL-8 gene and protein expressions were determined by RT-PCR and ELISA. Cell viability was determined by methyl thiazoyltetrazolium (MTT) assay. Phosphorylation of ERK1/2 and p38 were examined by western blotting. RESULTS: TMP inhibits LPS-induced IL-8 production in HUVECs at both the protein and mRNA levels, suggesting that TMP has an antiinflammatory effect on endothelial cells. TMP also inhibited U937 monocyte adhesion to HUVECs stimulated by LPS. LPS-induced phosphorylation of ERK1/2 and p38 were inhibited by TMP. The inhibitory effect of TMP on NF-kappaB (p65) activity was mediated by blocking the consequent translocation of p65 into the nucleus. CONCLUSIONS: The inhibitory effect of TMP on the LPS-induced IL-8 production is mediated by the NF-kappaB-dependent pathway, and TMP also separately affects the ERK and p38 MAPK pathway. TMP may be beneficial in the treatment of cardiovascular disorders such as atherosclerosis. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19540326/Tetramethylpyrazine_suppresses_interleukin_8_expression_in_LPS_stimulated_human_umbilical_vein_endothelial_cell_by_blocking_ERK_p38_and_nulear_factor_kappaB_signaling_pathways_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00372-9 DB - PRIME DP - Unbound Medicine ER -