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In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert.
Food Chem Toxicol 2009; 47(9):2338-43FC

Abstract

The aim of this study was to assess the in vitro potential of ethanolic leaf extract of Stevia rebaudiana as a natural antioxidant. The DPPH activity of the extract (20, 40, 50, 100 and 200 microg/ml) was increased in a dose dependent manner, which was found in the range of 36.93-68.76% as compared to ascorbic acid 64.26-82.58%. The IC(50) values of ethanolic extract and ascorbic acid in DPPH radical scavenging assay were obtained to be 93.46 and 26.75 microg/ml, respectively. The ethanolic extract was also found to scavenge the superoxide generated by EDTA/NBT system. Measurement of total phenolic content of the ethanolic extract of S. rebaudiana was achieved using Folin-Ciocalteau reagent containing 61.50 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The ethanolic extract also inhibited the hydroxyl radical, nitric oxide, superoxide anions with IC(50) values of 93.46, 132.05 and 81.08 microg/ml, respectively. However, the IC(50) values for the standard ascorbic acid were noted to be 26.75, 66.01 and 71.41 microg/ml respectively. The results obtained in this study clearly indicate that S. rebaudiana has a significant potential to use as a natural antioxidant agent.

Authors+Show Affiliations

Faculty of Life Sciences, Dr HS Gour University, Sagar, Madhya Pradesh, India. s.microb@yahoo.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19540900

Citation

Shukla, Shruti, et al. "In Vitro Antioxidant Activity and Total Phenolic Content of Ethanolic Leaf Extract of Stevia Rebaudiana Bert." Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, vol. 47, no. 9, 2009, pp. 2338-43.
Shukla S, Mehta A, Bajpai VK, et al. In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food Chem Toxicol. 2009;47(9):2338-43.
Shukla, S., Mehta, A., Bajpai, V. K., & Shukla, S. (2009). In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association, 47(9), pp. 2338-43. doi:10.1016/j.fct.2009.06.024.
Shukla S, et al. In Vitro Antioxidant Activity and Total Phenolic Content of Ethanolic Leaf Extract of Stevia Rebaudiana Bert. Food Chem Toxicol. 2009;47(9):2338-43. PubMed PMID: 19540900.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - In vitro antioxidant activity and total phenolic content of ethanolic leaf extract of Stevia rebaudiana Bert. AU - Shukla,Shruti, AU - Mehta,Archana, AU - Bajpai,Vivek K, AU - Shukla,Savita, Y1 - 2009/06/21/ PY - 2009/05/12/received PY - 2009/06/12/revised PY - 2009/06/15/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/10/14/medline SP - 2338 EP - 43 JF - Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association JO - Food Chem. Toxicol. VL - 47 IS - 9 N2 - The aim of this study was to assess the in vitro potential of ethanolic leaf extract of Stevia rebaudiana as a natural antioxidant. The DPPH activity of the extract (20, 40, 50, 100 and 200 microg/ml) was increased in a dose dependent manner, which was found in the range of 36.93-68.76% as compared to ascorbic acid 64.26-82.58%. The IC(50) values of ethanolic extract and ascorbic acid in DPPH radical scavenging assay were obtained to be 93.46 and 26.75 microg/ml, respectively. The ethanolic extract was also found to scavenge the superoxide generated by EDTA/NBT system. Measurement of total phenolic content of the ethanolic extract of S. rebaudiana was achieved using Folin-Ciocalteau reagent containing 61.50 mg/g of phenolic content, which was found significantly higher when compared to reference standard gallic acid. The ethanolic extract also inhibited the hydroxyl radical, nitric oxide, superoxide anions with IC(50) values of 93.46, 132.05 and 81.08 microg/ml, respectively. However, the IC(50) values for the standard ascorbic acid were noted to be 26.75, 66.01 and 71.41 microg/ml respectively. The results obtained in this study clearly indicate that S. rebaudiana has a significant potential to use as a natural antioxidant agent. SN - 1873-6351 UR - https://www.unboundmedicine.com/medline/citation/19540900/In_vitro_antioxidant_activity_and_total_phenolic_content_of_ethanolic_leaf_extract_of_Stevia_rebaudiana_Bert_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0278-6915(09)00298-1 DB - PRIME DP - Unbound Medicine ER -