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Expression and function of intestinal hexose transporters after small intestinal denervation.
Surgery 2009; 146(1):100-12S

Abstract

BACKGROUND

The role of neural regulation in expression and function of intestinal hexose transporters is unknown. The aim of this study is to determine the role of intestinal innervation in gene expression and function of the membrane hexose transporters, SGLT1, GLUT2, and GLUT5 in the enterocyte. We hypothesize that denervation of the small intestine decreases expression of hexose transporters, which leads to decreased glucose absorption.

METHODS

Six groups of Lewis rats were studied (n = 6 each) as follows: control, 1 week after sham laparotomy, 1 and 8 weeks after syngeneic (no immune rejection) orthotopic small-bowel transplantation (SBT) (SBT1 and SBT8) to induce complete extrinsic denervation, and 1 and 8 weeks after selective disruption of intrinsic neural continuity to jejunoileum by gut transection and reanastomosis (T/A1 and T/A8). All tissue was harvested between 8 AM and 10 AM. In duodenum, jejunum, and ileum, mucosal messenger RNA (mRNA) levels were quantitated by real-time polymerase chain reaction (PCR), protein by Western blotting, and transporter-mediated glucose absorption using the everted sleeve technique.

RESULTS

Across the 6 groups, the relative gene expression of hexose transporter mRNA and protein levels were unchanged, and no difference in transporter-mediated glucose uptake was evident in any region. The glucose transporter affinity (K(m)) and functional transporter levels (V(max)) calculated for duodenum and jejunum showed no difference among the 6 groups.

CONCLUSION

Baseline regulation of hexose transporter function is not mediated tonically by intrinsic or extrinsic neural continuity to the jejunoileum.

Authors+Show Affiliations

Department of Surgery, Mayo Clinic College of Medicine, Rochester, MN 55905, USA.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, N.I.H., Extramural

Language

eng

PubMed ID

19541015

Citation

Iqbal, Corey W., et al. "Expression and Function of Intestinal Hexose Transporters After Small Intestinal Denervation." Surgery, vol. 146, no. 1, 2009, pp. 100-12.
Iqbal CW, Fatima J, Duenes J, et al. Expression and function of intestinal hexose transporters after small intestinal denervation. Surgery. 2009;146(1):100-12.
Iqbal, C. W., Fatima, J., Duenes, J., Houghton, S. G., Kasparek, M. S., & Sarr, M. G. (2009). Expression and function of intestinal hexose transporters after small intestinal denervation. Surgery, 146(1), pp. 100-12. doi:10.1016/j.surg.2009.02.012.
Iqbal CW, et al. Expression and Function of Intestinal Hexose Transporters After Small Intestinal Denervation. Surgery. 2009;146(1):100-12. PubMed PMID: 19541015.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Expression and function of intestinal hexose transporters after small intestinal denervation. AU - Iqbal,Corey W, AU - Fatima,Javairiah, AU - Duenes,Judith, AU - Houghton,Scott G, AU - Kasparek,Michael S, AU - Sarr,Michael G, Y1 - 2009/05/08/ PY - 2008/09/24/received PY - 2009/02/23/accepted PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/7/7/medline SP - 100 EP - 12 JF - Surgery JO - Surgery VL - 146 IS - 1 N2 - BACKGROUND: The role of neural regulation in expression and function of intestinal hexose transporters is unknown. The aim of this study is to determine the role of intestinal innervation in gene expression and function of the membrane hexose transporters, SGLT1, GLUT2, and GLUT5 in the enterocyte. We hypothesize that denervation of the small intestine decreases expression of hexose transporters, which leads to decreased glucose absorption. METHODS: Six groups of Lewis rats were studied (n = 6 each) as follows: control, 1 week after sham laparotomy, 1 and 8 weeks after syngeneic (no immune rejection) orthotopic small-bowel transplantation (SBT) (SBT1 and SBT8) to induce complete extrinsic denervation, and 1 and 8 weeks after selective disruption of intrinsic neural continuity to jejunoileum by gut transection and reanastomosis (T/A1 and T/A8). All tissue was harvested between 8 AM and 10 AM. In duodenum, jejunum, and ileum, mucosal messenger RNA (mRNA) levels were quantitated by real-time polymerase chain reaction (PCR), protein by Western blotting, and transporter-mediated glucose absorption using the everted sleeve technique. RESULTS: Across the 6 groups, the relative gene expression of hexose transporter mRNA and protein levels were unchanged, and no difference in transporter-mediated glucose uptake was evident in any region. The glucose transporter affinity (K(m)) and functional transporter levels (V(max)) calculated for duodenum and jejunum showed no difference among the 6 groups. CONCLUSION: Baseline regulation of hexose transporter function is not mediated tonically by intrinsic or extrinsic neural continuity to the jejunoileum. SN - 1532-7361 UR - https://www.unboundmedicine.com/medline/citation/19541015/Expression_and_function_of_intestinal_hexose_transporters_after_small_intestinal_denervation_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-6060(09)00144-5 DB - PRIME DP - Unbound Medicine ER -