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Four orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana.
Plant Cell Physiol. 2009 Aug; 50(8):1425-38.PC

Abstract

Four AP1/AGL9 functional MADS box genes were characterized from the orchid (Oncidium Gower Ramsey). OMADS6 is a SEP3 ortholog, OMADS11 is a SEP1/2 ortholog, OMADS7 is an AGL6-like gene and OMADS10 is a putative paleoAP1 ortholog. The identity of these four genes was further supported by the presence of conserved motifs in the C-terminal regions of the proteins. OMADS6 showed an expression pattern different from SEP3 orthologs, with expression in the sepal, petal, lip and carpel, and was barely detected in the stamen. The expression pattern for OMADS11 was similar to OMADS6 and different from SEP1/2 orthologs since its expression was undetectable in the stamen. The expression pattern for OMADS7 was nearly identical to OMADS6. The similarities in the expression patterns of the SEP/AGL6-like genes OMADS6, 11 and 7 indicated that their transcriptional regulation is highly evolutionarily conserved in the orchid. Unlike OMADS6/11/7, OMADS10 was only expressed in vegetative leaves and in the lip and carpel of mature flowers, which distinguishes it from most genes in the SQUA subfamily. Ectopic expression of OMADS6, 11 or 7 caused extremely early flowering, whereas 35S::OMADS10 only caused moderately early flowering in transgenic Arabidopsis plants. In addition, flower organ conversions, such as carpelloid sepals and staminoid petals, were observed in 35S::OMADS6 and carpelloid sepals were produced in 35S::OMADS7, while flower organ conversions were not observed in 35S::OMADS11 or 35S::OMADS10 transgenic flowers. This result reveals possible functional diversification of the orchid AP1/AGL9 genes OMADS6, 11, 7 and 10 in regulating flower transition and formation.

Authors+Show Affiliations

Graduate Institute of Biotechnology, National Chung Hsing University, Taichung, Taiwan 40227, ROC. chyang@dragon.nchu.edu.twNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19541596

Citation

Chang, Yu-Yun, et al. "Four Orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS Box Genes Show Novel Expression Patterns and Cause Different Effects On Floral Transition and Formation in Arabidopsis Thaliana." Plant & Cell Physiology, vol. 50, no. 8, 2009, pp. 1425-38.
Chang YY, Chiu YF, Wu JW, et al. Four orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana. Plant Cell Physiol. 2009;50(8):1425-38.
Chang, Y. Y., Chiu, Y. F., Wu, J. W., & Yang, C. H. (2009). Four orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana. Plant & Cell Physiology, 50(8), 1425-38. https://doi.org/10.1093/pcp/pcp087
Chang YY, et al. Four Orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS Box Genes Show Novel Expression Patterns and Cause Different Effects On Floral Transition and Formation in Arabidopsis Thaliana. Plant Cell Physiol. 2009;50(8):1425-38. PubMed PMID: 19541596.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Four orchid (Oncidium Gower Ramsey) AP1/AGL9-like MADS box genes show novel expression patterns and cause different effects on floral transition and formation in Arabidopsis thaliana. AU - Chang,Yu-Yun, AU - Chiu,Yi-Feng, AU - Wu,Jia-Wei, AU - Yang,Chang-Hsien, Y1 - 2009/06/18/ PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/9/11/medline SP - 1425 EP - 38 JF - Plant & cell physiology JO - Plant Cell Physiol VL - 50 IS - 8 N2 - Four AP1/AGL9 functional MADS box genes were characterized from the orchid (Oncidium Gower Ramsey). OMADS6 is a SEP3 ortholog, OMADS11 is a SEP1/2 ortholog, OMADS7 is an AGL6-like gene and OMADS10 is a putative paleoAP1 ortholog. The identity of these four genes was further supported by the presence of conserved motifs in the C-terminal regions of the proteins. OMADS6 showed an expression pattern different from SEP3 orthologs, with expression in the sepal, petal, lip and carpel, and was barely detected in the stamen. The expression pattern for OMADS11 was similar to OMADS6 and different from SEP1/2 orthologs since its expression was undetectable in the stamen. The expression pattern for OMADS7 was nearly identical to OMADS6. The similarities in the expression patterns of the SEP/AGL6-like genes OMADS6, 11 and 7 indicated that their transcriptional regulation is highly evolutionarily conserved in the orchid. Unlike OMADS6/11/7, OMADS10 was only expressed in vegetative leaves and in the lip and carpel of mature flowers, which distinguishes it from most genes in the SQUA subfamily. Ectopic expression of OMADS6, 11 or 7 caused extremely early flowering, whereas 35S::OMADS10 only caused moderately early flowering in transgenic Arabidopsis plants. In addition, flower organ conversions, such as carpelloid sepals and staminoid petals, were observed in 35S::OMADS6 and carpelloid sepals were produced in 35S::OMADS7, while flower organ conversions were not observed in 35S::OMADS11 or 35S::OMADS10 transgenic flowers. This result reveals possible functional diversification of the orchid AP1/AGL9 genes OMADS6, 11, 7 and 10 in regulating flower transition and formation. SN - 1471-9053 UR - https://www.unboundmedicine.com/medline/citation/19541596/Four_orchid__Oncidium_Gower_Ramsey__AP1/AGL9_like_MADS_box_genes_show_novel_expression_patterns_and_cause_different_effects_on_floral_transition_and_formation_in_Arabidopsis_thaliana_ L2 - https://academic.oup.com/pcp/article-lookup/doi/10.1093/pcp/pcp087 DB - PRIME DP - Unbound Medicine ER -