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Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site.

Abstract

Intestinal nutrient transport is altered in response to changes in dietary conditions and luminal substrate level. It is not clear, however, whether an amino acid in the intestinal lumen can acutely affect its own absorption from a distant site. Our aim is to study the effect of an amino acid present in rat small intestinal segment on its own absorption from a proximal or distal site and elucidate the underlying mechanisms. The effect of instillation of alanine (Ala) in either jejunum or ileum on its own absorption at ileal or jejunal level was examined in vivo. The modulation of this intestinal regulatory loop by the following interventions was studied: tetrodotoxin (TTX) added to Ala, subdiaphragmatic vagotomy, chemical ablation of capsaicin-sensitive primary afferent (CSPA) fibers, and IV administration of calcitonin gene-related peptide (CGRP) antagonist. In addition, the kinetics of jejunal Ala absorption and the importance of Na+-dependent transport were studied in vitro after instilling Ala in the ileum. Basal jejunal Ala absorption [0.198 +/- 0.018 micromol x cm(-1) x 20 min(-1) (means +/- SD)] was significantly decreased with the instillation of 20 mM Ala in the ileum or in an adjacent distal jejunal segment (0.12 +/- 0.015; P < 0.0001 and 0.138 +/- 0.014; P < 0.002, respectively). Comparable inhibition was observed in the presence of proline in the ileum. Moreover, basal Ala absorption from the ileum (0.169 +/- 0.025) was significantly decreased by the presence of 20 mM Ala in the jejunum (0.103 +/- 0.027; P < 0.01). The inhibitory effect on jejunal Ala absorption was abolished by TTX, subdiaphragmatic vagotomy, neonatal capsaicin treatment, and CGRP antagonism. In vitro studies showed that Ala in the ileum affects Na+-mediated transport and increases K(m) without affecting Vmax. Intraluminal amino acids control their own absorption from a distant part of the intestine, by affecting the affinity of the Na+-mediated Ala transporter, through a neuronal mechanism that involves CSPA and CGRP.

Authors+Show Affiliations

Department of Physiology, American University of Beirut Medical Center, Beirut, Lebanon. fmourad@aub.edu.lbNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19541927

Citation

Mourad, Fadi H., et al. "Amino Acids in the Rat Intestinal Lumen Regulate Their Own Absorption From a Distant Intestinal Site." American Journal of Physiology. Gastrointestinal and Liver Physiology, vol. 297, no. 2, 2009, pp. G292-8.
Mourad FH, Barada KA, Khoury C, et al. Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site. Am J Physiol Gastrointest Liver Physiol. 2009;297(2):G292-8.
Mourad, F. H., Barada, K. A., Khoury, C., Hamdi, T., Saadé, N. E., & Nassar, C. F. (2009). Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site. American Journal of Physiology. Gastrointestinal and Liver Physiology, 297(2), pp. G292-8. doi:10.1152/ajpgi.00100.2009.
Mourad FH, et al. Amino Acids in the Rat Intestinal Lumen Regulate Their Own Absorption From a Distant Intestinal Site. Am J Physiol Gastrointest Liver Physiol. 2009;297(2):G292-8. PubMed PMID: 19541927.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Amino acids in the rat intestinal lumen regulate their own absorption from a distant intestinal site. AU - Mourad,Fadi H, AU - Barada,Kassem A, AU - Khoury,Carmen, AU - Hamdi,Tamim, AU - Saadé,Nayef E, AU - Nassar,Camille F, Y1 - 2009/06/18/ PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/8/26/medline SP - G292 EP - 8 JF - American journal of physiology. Gastrointestinal and liver physiology JO - Am. J. Physiol. Gastrointest. Liver Physiol. VL - 297 IS - 2 N2 - Intestinal nutrient transport is altered in response to changes in dietary conditions and luminal substrate level. It is not clear, however, whether an amino acid in the intestinal lumen can acutely affect its own absorption from a distant site. Our aim is to study the effect of an amino acid present in rat small intestinal segment on its own absorption from a proximal or distal site and elucidate the underlying mechanisms. The effect of instillation of alanine (Ala) in either jejunum or ileum on its own absorption at ileal or jejunal level was examined in vivo. The modulation of this intestinal regulatory loop by the following interventions was studied: tetrodotoxin (TTX) added to Ala, subdiaphragmatic vagotomy, chemical ablation of capsaicin-sensitive primary afferent (CSPA) fibers, and IV administration of calcitonin gene-related peptide (CGRP) antagonist. In addition, the kinetics of jejunal Ala absorption and the importance of Na+-dependent transport were studied in vitro after instilling Ala in the ileum. Basal jejunal Ala absorption [0.198 +/- 0.018 micromol x cm(-1) x 20 min(-1) (means +/- SD)] was significantly decreased with the instillation of 20 mM Ala in the ileum or in an adjacent distal jejunal segment (0.12 +/- 0.015; P < 0.0001 and 0.138 +/- 0.014; P < 0.002, respectively). Comparable inhibition was observed in the presence of proline in the ileum. Moreover, basal Ala absorption from the ileum (0.169 +/- 0.025) was significantly decreased by the presence of 20 mM Ala in the jejunum (0.103 +/- 0.027; P < 0.01). The inhibitory effect on jejunal Ala absorption was abolished by TTX, subdiaphragmatic vagotomy, neonatal capsaicin treatment, and CGRP antagonism. In vitro studies showed that Ala in the ileum affects Na+-mediated transport and increases K(m) without affecting Vmax. Intraluminal amino acids control their own absorption from a distant part of the intestine, by affecting the affinity of the Na+-mediated Ala transporter, through a neuronal mechanism that involves CSPA and CGRP. SN - 1522-1547 UR - https://www.unboundmedicine.com/medline/citation/19541927/Amino_acids_in_the_rat_intestinal_lumen_regulate_their_own_absorption_from_a_distant_intestinal_site_ L2 - http://www.physiology.org/doi/full/10.1152/ajpgi.00100.2009?url_ver=Z39.88-2003&amp;rfr_id=ori:rid:crossref.org&amp;rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -