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Cloning and spatio-temporal expression of porcine CDK5 and CDK5R1(p35) genes.
Anim Biotechnol. 2009; 20(3):133-43.AB

Abstract

Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase homologue attributed to the mitotic cyclin-dependent kinase family. Both the kinase activity and the biological effects of CDK5 in central nervous system are mainly dependent on association with its regulatory subunit 1 known as CDK5R1 (p35). In the present study, the full-length coding regions of CDK5 and CDK5R1 were cloned from pigs. Radiation hybrid mapping localized porcine CDK5 to chromosome 18q12-13, whereas CDK5R1 was electro-localized to chromosome 12q12. Real-time quantitative RT-PCR (qRT-PCR) showed that CDK5 mRNA is ubiquitously present in all porcine tissues examined, with relatively high levels in cerebral cortex, cerebellum, testicle and lung. We also examined the expression profile of porcine CDK5/CDK5R1 in various tissues at different developmental stages. The results indicated that CDK5 mRNA reaches the highest level in cerebral cortex at two months of age and in cerebellum and liver at 4 months of age, respectively, whereas the peak level of CDK5R1 was observed in both cerebral cortex and cerebellum at two months of age, indicating the pivotal role of CDK5/CDK5R1 during the development of porcine brain.

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Authors+Show Affiliations

Long H
Key Laboratory of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education, College of Life Science and Technology, Huazhong Agricultural University, Wuhan, P.R. China.
Zhao S
No affiliation info available
Lei T
No affiliation info available
Han J
No affiliation info available
Yuan J
No affiliation info available
Qi Y
No affiliation info available
Yang Z
No affiliation info available

MeSH

Amino Acid SequenceAnimalsBase SequenceBrainCloning, MolecularCyclin-Dependent Kinase 5DNA PrimersGene Expression Regulation, DevelopmentalMaleMolecular Sequence DataNerve Tissue ProteinsRNA, MessengerRadiation Hybrid MappingSus scrofaTissue Distribution

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19544209

Citation

Long, Huan, et al. "Cloning and Spatio-temporal Expression of Porcine CDK5 and CDK5R1(p35) Genes." Animal Biotechnology, vol. 20, no. 3, 2009, pp. 133-43.
Long H, Zhao S, Lei T, et al. Cloning and spatio-temporal expression of porcine CDK5 and CDK5R1(p35) genes. Anim Biotechnol. 2009;20(3):133-43.
Long, H., Zhao, S., Lei, T., Han, J., Yuan, J., Qi, Y., & Yang, Z. (2009). Cloning and spatio-temporal expression of porcine CDK5 and CDK5R1(p35) genes. Animal Biotechnology, 20(3), 133-43. https://doi.org/10.1080/10495390902952841
Long H, et al. Cloning and Spatio-temporal Expression of Porcine CDK5 and CDK5R1(p35) Genes. Anim Biotechnol. 2009;20(3):133-43. PubMed PMID: 19544209.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Cloning and spatio-temporal expression of porcine CDK5 and CDK5R1(p35) genes. AU - Long,Huan, AU - Zhao,Shanting, AU - Lei,Ting, AU - Han,Jichao, AU - Yuan,Jihong, AU - Qi,Yanmei, AU - Yang,Zaiqing, PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/10/3/medline SP - 133 EP - 43 JF - Animal biotechnology JO - Anim Biotechnol VL - 20 IS - 3 N2 - Cyclin-dependent kinase 5 (CDK5) is a serine/threonine kinase homologue attributed to the mitotic cyclin-dependent kinase family. Both the kinase activity and the biological effects of CDK5 in central nervous system are mainly dependent on association with its regulatory subunit 1 known as CDK5R1 (p35). In the present study, the full-length coding regions of CDK5 and CDK5R1 were cloned from pigs. Radiation hybrid mapping localized porcine CDK5 to chromosome 18q12-13, whereas CDK5R1 was electro-localized to chromosome 12q12. Real-time quantitative RT-PCR (qRT-PCR) showed that CDK5 mRNA is ubiquitously present in all porcine tissues examined, with relatively high levels in cerebral cortex, cerebellum, testicle and lung. We also examined the expression profile of porcine CDK5/CDK5R1 in various tissues at different developmental stages. The results indicated that CDK5 mRNA reaches the highest level in cerebral cortex at two months of age and in cerebellum and liver at 4 months of age, respectively, whereas the peak level of CDK5R1 was observed in both cerebral cortex and cerebellum at two months of age, indicating the pivotal role of CDK5/CDK5R1 during the development of porcine brain. SN - 1532-2378 UR - https://www.unboundmedicine.com/medline/citation/19544209/Cloning_and_spatio_temporal_expression_of_porcine_CDK5_and_CDK5R1_p35__genes_ L2 - https://www.tandfonline.com/doi/full/10.1080/10495390902952841 DB - PRIME DP - Unbound Medicine ER -