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Enrichment of anaerobic methanotrophs in sulfate-reducing membrane bioreactors.
Biotechnol Bioeng. 2009 Oct 15; 104(3):458-70.BB

Abstract

Anaerobic oxidation of methane (AOM) in marine sediments is coupled to sulfate reduction (SR). AOM is mediated by distinct groups of archaea, called anaerobic methanotrophs (ANME). ANME co-exist with sulfate-reducing bacteria, which are also involved in AOM coupled SR. The microorganisms involved in AOM coupled to SR are extremely difficult to grow in vitro. Here, a novel well-mixed submerged-membrane bioreactor system is used to grow and enrich the microorganisms mediating AOM coupled to SR. Four reactors were inoculated with sediment sampled in the Eckernförde Bay (Baltic Sea) and operated at a methane and sulfate loading rate of 4.8 L L(-1) day(-1) (196 mmol L(-1) day(-1)) and 3.0 mmol L(-1) day(-1). Two bioreactors were controlled at 15 degrees C and two at 30 degrees C, one reactor at 30 degrees C contained also anaerobic granular sludge. At 15 degrees C, the volumetric AOM and SR rates doubled approximately every 3.8 months. After 884 days, an enrichment culture was obtained with an AOM and SR rate of 1.0 mmol g(volatile suspended solids) (-1) day(-1) (286 micromol g(dry weight) (-1) day(-1)). No increase in AOM and SR was observed in the two bioreactors operated at 30 degrees C. The microbial community of one of the 15 degrees C reactors was analyzed. ANME-2a became the dominant archaea. This study showed that sulfate reduction with methane as electron donor is possible in well-mixed bioreactors and that the submerged-membrane bioreactor system is an excellent system to enrich slow-growing microorganisms, like methanotrophic archaea.

Authors+Show Affiliations

Sub-department of Environmental Technology, Wageningen University, Bomenweg 2, 6703 HD Wageningen, The Netherlands. r.meulepas@unesco-ihe.orgNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19544305

Citation

Meulepas, Roel J W., et al. "Enrichment of Anaerobic Methanotrophs in Sulfate-reducing Membrane Bioreactors." Biotechnology and Bioengineering, vol. 104, no. 3, 2009, pp. 458-70.
Meulepas RJ, Jagersma CG, Gieteling J, et al. Enrichment of anaerobic methanotrophs in sulfate-reducing membrane bioreactors. Biotechnol Bioeng. 2009;104(3):458-70.
Meulepas, R. J., Jagersma, C. G., Gieteling, J., Buisman, C. J., Stams, A. J., & Lens, P. N. (2009). Enrichment of anaerobic methanotrophs in sulfate-reducing membrane bioreactors. Biotechnology and Bioengineering, 104(3), 458-70. https://doi.org/10.1002/bit.22412
Meulepas RJ, et al. Enrichment of Anaerobic Methanotrophs in Sulfate-reducing Membrane Bioreactors. Biotechnol Bioeng. 2009 Oct 15;104(3):458-70. PubMed PMID: 19544305.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Enrichment of anaerobic methanotrophs in sulfate-reducing membrane bioreactors. AU - Meulepas,Roel J W, AU - Jagersma,Christian G, AU - Gieteling,Jarno, AU - Buisman,Cees J N, AU - Stams,Alfons J M, AU - Lens,Piet N L, PY - 2009/6/23/entrez PY - 2009/6/23/pubmed PY - 2009/10/23/medline SP - 458 EP - 70 JF - Biotechnology and bioengineering JO - Biotechnol. Bioeng. VL - 104 IS - 3 N2 - Anaerobic oxidation of methane (AOM) in marine sediments is coupled to sulfate reduction (SR). AOM is mediated by distinct groups of archaea, called anaerobic methanotrophs (ANME). ANME co-exist with sulfate-reducing bacteria, which are also involved in AOM coupled SR. The microorganisms involved in AOM coupled to SR are extremely difficult to grow in vitro. Here, a novel well-mixed submerged-membrane bioreactor system is used to grow and enrich the microorganisms mediating AOM coupled to SR. Four reactors were inoculated with sediment sampled in the Eckernförde Bay (Baltic Sea) and operated at a methane and sulfate loading rate of 4.8 L L(-1) day(-1) (196 mmol L(-1) day(-1)) and 3.0 mmol L(-1) day(-1). Two bioreactors were controlled at 15 degrees C and two at 30 degrees C, one reactor at 30 degrees C contained also anaerobic granular sludge. At 15 degrees C, the volumetric AOM and SR rates doubled approximately every 3.8 months. After 884 days, an enrichment culture was obtained with an AOM and SR rate of 1.0 mmol g(volatile suspended solids) (-1) day(-1) (286 micromol g(dry weight) (-1) day(-1)). No increase in AOM and SR was observed in the two bioreactors operated at 30 degrees C. The microbial community of one of the 15 degrees C reactors was analyzed. ANME-2a became the dominant archaea. This study showed that sulfate reduction with methane as electron donor is possible in well-mixed bioreactors and that the submerged-membrane bioreactor system is an excellent system to enrich slow-growing microorganisms, like methanotrophic archaea. SN - 1097-0290 UR - https://www.unboundmedicine.com/medline/citation/19544305/Enrichment_of_anaerobic_methanotrophs_in_sulfate_reducing_membrane_bioreactors_ L2 - https://doi.org/10.1002/bit.22412 DB - PRIME DP - Unbound Medicine ER -