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Hypergravity modulates vitamin D receptor target gene mRNA expression in mice.
Am J Physiol Endocrinol Metab. 2009 Sep; 297(3):E728-34.AJ

Abstract

The possibility of pathological calcium metabolism is a critical health concern introduced by long-term space travel. Because vitamin D plays an important role in calcium homeostasis, we evaluated the effects of hypergravity on the expression of genes involved in vitamin D and calcium metabolism in ICR mice. When exposed to 2G hypergravity for 2 days, the mRNA expression of renal 25-hydroxyvitamin D 24-hydroxylase (Cyp24a1) was increased and that of 25-hydroxyvitamin D 1alpha-hydroxylase (Cyp27b1) was decreased. Although hypergravity decreased food intake and increased the expression of starvation-induced genes, the changes in Cyp24a1 and Cyp27b1 expression were not due to starvation, suggesting that hypergravity affects these genes directly. Hypergravity decreased plasma 1alpha,25-dihydroxyvitamin D(3) levels in ICR mice, suggesting a consequence of decreased Cyp27b1 and increased Cyp24a1 expression. Although 1alpha-hydroxyvitamin D(3) [1alpha(OH)D(3)] treatment induced the expression of vitamin D receptor (VDR) target genes in the kidney of 2G-exposed ICR mice to similar levels as controls, 1alpha(OH)D(3) increased the intestinal expression of Cyp24a1 in ICR mice. Hypergravity-dependent changes of Cyp24a1 and Cyp27b1 expression were diminished in mice exposed to hypergravity for 14 days, which may represent an adaptation to hypergravity stress. Hypergravity exposure also increased Cyp24a1 expression in the kidney of C57BL/6J mice. We examined the effects of hypergravity on VDR-null mice and found that renal Cyp27b1 expression in VDR-null mice was decreased by hypergravity while renal Cyp24a1 expression was not detected in VDR-null mice. Thus hypergravity modifies the expression of genes involved in vitamin D metabolism.

Authors+Show Affiliations

Division of Biochemistry, Dept. of Biomedical Sciences, Nihon Univ. School of Medicine, 30-1 Oyaguchi-kamicho, Itabashi-ku, Tokyo 173-8610, Japan.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19549793

Citation

Ishizawa, Michiyasu, et al. "Hypergravity Modulates Vitamin D Receptor Target Gene mRNA Expression in Mice." American Journal of Physiology. Endocrinology and Metabolism, vol. 297, no. 3, 2009, pp. E728-34.
Ishizawa M, Iwasaki K, Kato S, et al. Hypergravity modulates vitamin D receptor target gene mRNA expression in mice. Am J Physiol Endocrinol Metab. 2009;297(3):E728-34.
Ishizawa, M., Iwasaki, K., Kato, S., & Makishima, M. (2009). Hypergravity modulates vitamin D receptor target gene mRNA expression in mice. American Journal of Physiology. Endocrinology and Metabolism, 297(3), E728-34. https://doi.org/10.1152/ajpendo.00168.2009
Ishizawa M, et al. Hypergravity Modulates Vitamin D Receptor Target Gene mRNA Expression in Mice. Am J Physiol Endocrinol Metab. 2009;297(3):E728-34. PubMed PMID: 19549793.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Hypergravity modulates vitamin D receptor target gene mRNA expression in mice. AU - Ishizawa,Michiyasu, AU - Iwasaki,Ken-Ichi, AU - Kato,Shigeaki, AU - Makishima,Makoto, Y1 - 2009/06/23/ PY - 2009/6/25/entrez PY - 2009/6/25/pubmed PY - 2009/10/1/medline SP - E728 EP - 34 JF - American journal of physiology. Endocrinology and metabolism JO - Am. J. Physiol. Endocrinol. Metab. VL - 297 IS - 3 N2 - The possibility of pathological calcium metabolism is a critical health concern introduced by long-term space travel. Because vitamin D plays an important role in calcium homeostasis, we evaluated the effects of hypergravity on the expression of genes involved in vitamin D and calcium metabolism in ICR mice. When exposed to 2G hypergravity for 2 days, the mRNA expression of renal 25-hydroxyvitamin D 24-hydroxylase (Cyp24a1) was increased and that of 25-hydroxyvitamin D 1alpha-hydroxylase (Cyp27b1) was decreased. Although hypergravity decreased food intake and increased the expression of starvation-induced genes, the changes in Cyp24a1 and Cyp27b1 expression were not due to starvation, suggesting that hypergravity affects these genes directly. Hypergravity decreased plasma 1alpha,25-dihydroxyvitamin D(3) levels in ICR mice, suggesting a consequence of decreased Cyp27b1 and increased Cyp24a1 expression. Although 1alpha-hydroxyvitamin D(3) [1alpha(OH)D(3)] treatment induced the expression of vitamin D receptor (VDR) target genes in the kidney of 2G-exposed ICR mice to similar levels as controls, 1alpha(OH)D(3) increased the intestinal expression of Cyp24a1 in ICR mice. Hypergravity-dependent changes of Cyp24a1 and Cyp27b1 expression were diminished in mice exposed to hypergravity for 14 days, which may represent an adaptation to hypergravity stress. Hypergravity exposure also increased Cyp24a1 expression in the kidney of C57BL/6J mice. We examined the effects of hypergravity on VDR-null mice and found that renal Cyp27b1 expression in VDR-null mice was decreased by hypergravity while renal Cyp24a1 expression was not detected in VDR-null mice. Thus hypergravity modifies the expression of genes involved in vitamin D metabolism. SN - 1522-1555 UR - https://www.unboundmedicine.com/medline/citation/19549793/Hypergravity_modulates_vitamin_D_receptor_target_gene_mRNA_expression_in_mice_ L2 - http://www.physiology.org/doi/full/10.1152/ajpendo.00168.2009?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -