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Simultaneous determination of fluoroquinolones, sulfonamides and tetracyclines in sewage sludge by pressurized liquid extraction and liquid chromatography electrospray ionization-mass spectrometry.
J Chromatogr A. 2009 Aug 07; 1216(32):5949-54.JC

Abstract

A new scheme for the quantitative determination of traces of fluoroquinolones (FQs), tetracyclines (TCs) and sulfonamides (SAs) in sewage sludge was developed. The compounds were simultaneously extracted from sewage sludge by pressurized liquid extraction (PLE). A novel and effective method for PLE was developed. Solid-phase extraction was used for cleaning up the extracts. Identification and quantification of the compounds was done using high-performance liquid chromatography with electrospray ionization mass spectrometry in selected reaction monitoring mode. The best recovery of FQs and TCs was obtained by using hydrophilic-lipophilic balance cartridges, recoveries ranged 59% for norfloxacin to 82% for ofloxacin and 95% for doxycycline; for SAs strong cation-exchange cartridges were more efficient, recoveries were 96% for sulfamethoxazole and 43% for sulfadimethoxine. Limit of quantification ranged from 0.1 ng/g for SAs to 160 ng/g for tetracycline. Method precision for TCs was 5.06% and 1.12%, and for SAs 0.43% and 2.01%. FQs precision ranged from 0.77% to 1.89%.

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Authors+Show Affiliations

Lillenberg M
Department of Food Science and Hygiene, Estonian University of Life Sciences, Kreutzwaldi 58A, 51014 Tartu, Estonia.
Yurchenko S
No affiliation info available
Kipper K
No affiliation info available
Herodes K
No affiliation info available
Pihl V
No affiliation info available
Sepp K
No affiliation info available
Lõhmus R
No affiliation info available
Nei L
No affiliation info available

MeSH

Chemical FractionationChromatography, LiquidEquipment DesignFluoroquinolonesPressureReproducibility of ResultsSensitivity and SpecificitySewageSolid Phase ExtractionSpectrometry, Mass, Electrospray IonizationSulfonamidesTetracyclines

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19552910

Citation

Lillenberg, Merike, et al. "Simultaneous Determination of Fluoroquinolones, Sulfonamides and Tetracyclines in Sewage Sludge By Pressurized Liquid Extraction and Liquid Chromatography Electrospray Ionization-mass Spectrometry." Journal of Chromatography. A, vol. 1216, no. 32, 2009, pp. 5949-54.
Lillenberg M, Yurchenko S, Kipper K, et al. Simultaneous determination of fluoroquinolones, sulfonamides and tetracyclines in sewage sludge by pressurized liquid extraction and liquid chromatography electrospray ionization-mass spectrometry. J Chromatogr A. 2009;1216(32):5949-54.
Lillenberg, M., Yurchenko, S., Kipper, K., Herodes, K., Pihl, V., Sepp, K., Lõhmus, R., & Nei, L. (2009). Simultaneous determination of fluoroquinolones, sulfonamides and tetracyclines in sewage sludge by pressurized liquid extraction and liquid chromatography electrospray ionization-mass spectrometry. Journal of Chromatography. A, 1216(32), 5949-54. https://doi.org/10.1016/j.chroma.2009.06.029
Lillenberg M, et al. Simultaneous Determination of Fluoroquinolones, Sulfonamides and Tetracyclines in Sewage Sludge By Pressurized Liquid Extraction and Liquid Chromatography Electrospray Ionization-mass Spectrometry. J Chromatogr A. 2009 Aug 7;1216(32):5949-54. PubMed PMID: 19552910.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous determination of fluoroquinolones, sulfonamides and tetracyclines in sewage sludge by pressurized liquid extraction and liquid chromatography electrospray ionization-mass spectrometry. AU - Lillenberg,Merike, AU - Yurchenko,Sergei, AU - Kipper,Karin, AU - Herodes,Koit, AU - Pihl,Viljar, AU - Sepp,Kalev, AU - Lõhmus,Rünno, AU - Nei,Lembit, Y1 - 2009/06/13/ PY - 2009/03/06/received PY - 2009/06/08/revised PY - 2009/06/10/accepted PY - 2009/6/26/entrez PY - 2009/6/26/pubmed PY - 2009/9/4/medline SP - 5949 EP - 54 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 32 N2 - A new scheme for the quantitative determination of traces of fluoroquinolones (FQs), tetracyclines (TCs) and sulfonamides (SAs) in sewage sludge was developed. The compounds were simultaneously extracted from sewage sludge by pressurized liquid extraction (PLE). A novel and effective method for PLE was developed. Solid-phase extraction was used for cleaning up the extracts. Identification and quantification of the compounds was done using high-performance liquid chromatography with electrospray ionization mass spectrometry in selected reaction monitoring mode. The best recovery of FQs and TCs was obtained by using hydrophilic-lipophilic balance cartridges, recoveries ranged 59% for norfloxacin to 82% for ofloxacin and 95% for doxycycline; for SAs strong cation-exchange cartridges were more efficient, recoveries were 96% for sulfamethoxazole and 43% for sulfadimethoxine. Limit of quantification ranged from 0.1 ng/g for SAs to 160 ng/g for tetracycline. Method precision for TCs was 5.06% and 1.12%, and for SAs 0.43% and 2.01%. FQs precision ranged from 0.77% to 1.89%. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19552910/Simultaneous_determination_of_fluoroquinolones_sulfonamides_and_tetracyclines_in_sewage_sludge_by_pressurized_liquid_extraction_and_liquid_chromatography_electrospray_ionization_mass_spectrometry_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00906-6 DB - PRIME DP - Unbound Medicine ER -
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