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Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses.
Clin Vaccine Immunol. 2009 Aug; 16(8):1132-8.CV

Abstract

Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus.

Authors+Show Affiliations

Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19553554

Citation

Nakauchi, Mina, et al. "Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused By South American Arenaviruses." Clinical and Vaccine Immunology : CVI, vol. 16, no. 8, 2009, pp. 1132-8.
Nakauchi M, Fukushi S, Saijo M, et al. Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses. Clin Vaccine Immunol. 2009;16(8):1132-8.
Nakauchi, M., Fukushi, S., Saijo, M., Mizutani, T., Ure, A. E., Romanowski, V., Kurane, I., & Morikawa, S. (2009). Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses. Clinical and Vaccine Immunology : CVI, 16(8), 1132-8. https://doi.org/10.1128/CVI.00163-09
Nakauchi M, et al. Characterization of Monoclonal Antibodies to Junin Virus Nucleocapsid Protein and Application to the Diagnosis of Hemorrhagic Fever Caused By South American Arenaviruses. Clin Vaccine Immunol. 2009;16(8):1132-8. PubMed PMID: 19553554.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Characterization of monoclonal antibodies to Junin virus nucleocapsid protein and application to the diagnosis of hemorrhagic fever caused by South American arenaviruses. AU - Nakauchi,Mina, AU - Fukushi,Shuetsu, AU - Saijo,Masayuki, AU - Mizutani,Tetsuya, AU - Ure,Agustín E, AU - Romanowski,Victor, AU - Kurane,Ichiro, AU - Morikawa,Shigeru, Y1 - 2009/06/24/ PY - 2009/6/26/entrez PY - 2009/6/26/pubmed PY - 2009/9/29/medline SP - 1132 EP - 8 JF - Clinical and vaccine immunology : CVI JO - Clin Vaccine Immunol VL - 16 IS - 8 N2 - Junin virus (JUNV), Machupo virus, Guanarito virus, Sabia virus, and Chapare virus are members of New World arenavirus clade B and are the etiological agents of viral hemorrhagic fevers that occur in South America. In this study, we produced three monoclonal antibodies (MAbs) to the recombinant nucleocapsid protein of JUNV, designated C6-9, C11-12, and E4-2. The specificity of these MAbs was examined by enzyme-linked immunosorbent assay (ELISA), indirect immunofluorescence assay, and an epitope-mapping method. Using these MAbs, we developed antigen (Ag) capture ELISA systems. We showed that by using MAb C6-9, JUNV Ag was specifically detected. On the other hand, by using MAb C11-12 or E-4-2, the Ags of all human pathogenic South American arenaviruses were detected. The combined use of these Ag capture ELISA systems in the present study may be useful for the diagnosis of acute-phase viral hemorrhagic fever due to infection by a South American arenavirus. SN - 1556-679X UR - https://www.unboundmedicine.com/medline/citation/19553554/Characterization_of_monoclonal_antibodies_to_Junin_virus_nucleocapsid_protein_and_application_to_the_diagnosis_of_hemorrhagic_fever_caused_by_South_American_arenaviruses_ L2 - https://journals.asm.org/doi/10.1128/CVI.00163-09?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub=pubmed DB - PRIME DP - Unbound Medicine ER -