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Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans.

Abstract

Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F(2)-isoprostanes (F(2)-IsoPs) and F(4)-neuroprostanes (F(4)-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F(2)-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F(4)-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F(4)-NPs in CSF. [(2)H(4)]-15-F(2t)-IsoP, rather than [(18)O(2)]-17-F(4c)-NP or [(2)H(4)]-PGF(2 alpha), was used as the internal standard for F(4)-NPs analysis. One problem of the use of [(18)O(2)]-17-F(4c)-NP was the potential interference resulting from F(2)-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F(4)-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F(4)-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F(4)-NPs in CSF could be a better predictor for outcome of aSAH than F(2)-IsoPs at early time points.

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  • Authors+Show Affiliations

    ,

    Graduate Institute of Medical Biotechnology/Department of Medical Biotechnology and Laboratory Science, College of Medicine, Chang Gung University, Tao-Yuan, Taiwan.

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    Source

    Free radical biology & medicine 47:6 2009 Sep 15 pg 814-24

    MeSH

    Adult
    Aged
    Aged, 80 and over
    Biomarkers
    Disease Progression
    Docosahexaenoic Acids
    Early Diagnosis
    F2-Isoprostanes
    Female
    Humans
    Lipid Peroxidation
    Male
    Middle Aged
    Oxidative Stress
    Prognosis
    Severity of Illness Index
    Subarachnoid Hemorrhage

    Pub Type(s)

    Journal Article
    Research Support, N.I.H., Extramural
    Research Support, Non-U.S. Gov't

    Language

    eng

    PubMed ID

    19555757

    Citation

    Hsieh, Yu-Ping, et al. "Correlation of F4-neuroprostanes Levels in Cerebrospinal Fluid With Outcome of Aneurysmal Subarachnoid Hemorrhage in Humans." Free Radical Biology & Medicine, vol. 47, no. 6, 2009, pp. 814-24.
    Hsieh YP, Lin CL, Shiue AL, et al. Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans. Free Radic Biol Med. 2009;47(6):814-24.
    Hsieh, Y. P., Lin, C. L., Shiue, A. L., Yin, H., Morrow, J. D., Hsu, J. C., ... Yen, H. C. (2009). Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans. Free Radical Biology & Medicine, 47(6), pp. 814-24. doi:10.1016/j.freeradbiomed.2009.06.026.
    Hsieh YP, et al. Correlation of F4-neuroprostanes Levels in Cerebrospinal Fluid With Outcome of Aneurysmal Subarachnoid Hemorrhage in Humans. Free Radic Biol Med. 2009 Sep 15;47(6):814-24. PubMed PMID: 19555757.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Correlation of F4-neuroprostanes levels in cerebrospinal fluid with outcome of aneurysmal subarachnoid hemorrhage in humans. AU - Hsieh,Yu-Ping, AU - Lin,Chih-Lung, AU - Shiue,An-Li, AU - Yin,Huiyong, AU - Morrow,Jason D, AU - Hsu,Jee-Ching, AU - Hsieh,Tsung-Che, AU - Wei,Hsing-Ju, AU - Yen,Hsiu-Chuan, Y1 - 2009/06/23/ PY - 2009/02/23/received PY - 2009/05/27/revised PY - 2009/06/18/accepted PY - 2009/6/27/entrez PY - 2009/6/27/pubmed PY - 2010/2/2/medline SP - 814 EP - 24 JF - Free radical biology & medicine JO - Free Radic. Biol. Med. VL - 47 IS - 6 N2 - Aneurysmal subarachnoid hemorrhage (aSAH) is one type of hemorrhagic stroke in humans. F(2)-isoprostanes (F(2)-IsoPs) and F(4)-neuroprostanes (F(4)-NPs), derived from arachidonic acid and docosahexaenoic acid (DHA), respectively, are specific markers of lipid peroxidation. We previously demonstrated that F(2)-IsoPs levels in cerebrospinal fluid (CSF) of aSAH patients positively correlated with poor clinical conditions. In this work, we refined F(4)-NPs analysis and investigated the role of potential oxidative damage to neurons in aSAH patients by detecting F(4)-NPs in CSF. [(2)H(4)]-15-F(2t)-IsoP, rather than [(18)O(2)]-17-F(4c)-NP or [(2)H(4)]-PGF(2 alpha), was used as the internal standard for F(4)-NPs analysis. One problem of the use of [(18)O(2)]-17-F(4c)-NP was the potential interference resulting from F(2)-dihomo-IsoPs in CSF. CSF specimens of 15 aSAH patients for up to 10 days and those of 12 non-aSAH controls were analyzed. First day, mean, and peak levels of F(4)-NPs were all significantly higher in aSAH patients than in controls and correlated with the Fisher Scale and 3-month Glasgow Outcome Scale, but only mean levels of F(4)-NPs correlated with Hunt and Hess Grade. The results first demonstrate oxidative damage to DHA in brain tissue following aSAH and suggest that F(4)-NPs in CSF could be a better predictor for outcome of aSAH than F(2)-IsoPs at early time points. SN - 1873-4596 UR - https://www.unboundmedicine.com/medline/citation/19555757/Correlation_of_F4_neuroprostanes_levels_in_cerebrospinal_fluid_with_outcome_of_aneurysmal_subarachnoid_hemorrhage_in_humans_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0891-5849(09)00377-3 DB - PRIME DP - Unbound Medicine ER -