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Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and comet assay.
Arch Environ Contam Toxicol. 2010 Jan; 58(1):123-30.AE

Abstract

Malathion [S-(1,2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate] is a widely used organophosphorus insecticide throughout the world. However, limited efforts have made to study its genotoxic effect in different fish tissues. The present investigation was aimed to assess the genotoxic potential of the pesticide to the freshwater teleost fish Channa punctatus at sublethal concentrations using the micronucleus test and comet assay. Initially, the 96-h LC50 value of commercial-grade malathion (50% EC) was determined as 5.93 ppm in a semistatic system. Based on LC50, three test concentrations (viz. sublethal I, sublethal II, and sublethal III) were determined to be 1.48, 0.74, and 0.59 ppm, respectively, and the fish specimens were exposed to these concentrations. Tissue samplings were done on days 0, 1, 3, 7, 15, 22 and 29 of malathion exposure for assessment of the induction of micronuclei (MN) frequency and DNA damage. The MN formation in the peripheral blood cells was found to be significantly higher (p < 0.05) in the treated specimens at all sampling intervals compared to the control. The MN frequency reached maximum on days 3 and 7 at sublethal I and II concentrations, respectively, followed by a nonlinear decline with the progression of the experiment. Similarly, significant effects (p < 0.05) of both concentration and time of exposure were observed on DNA damage in the gill, kidney, and lymphocytes. All of the tissues exhibited a concentration-dependent increase in DNA damage up to day 3, followed by a nonlinear decrease with the duration of exposure. A comparison of the extent of DNA damage among the tissues showed the sensitivity of gill tissue to malathion.

Authors+Show Affiliations

National Bureau of Fish Genetics Resources, Canal Ring Road, P O-Dilkusha, Lucknow 226002, India. Ravindra.Scientist@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19557474

Citation

Kumar, Ravindra, et al. "Investigation of the Genotoxicity of Malathion to Freshwater Teleost Fish Channa Punctatus (Bloch) Using the Micronucleus Test and Comet Assay." Archives of Environmental Contamination and Toxicology, vol. 58, no. 1, 2010, pp. 123-30.
Kumar R, Nagpure NS, Kushwaha B, et al. Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and comet assay. Arch Environ Contam Toxicol. 2010;58(1):123-30.
Kumar, R., Nagpure, N. S., Kushwaha, B., Srivastava, S. K., & Lakra, W. S. (2010). Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and comet assay. Archives of Environmental Contamination and Toxicology, 58(1), 123-30. https://doi.org/10.1007/s00244-009-9354-3
Kumar R, et al. Investigation of the Genotoxicity of Malathion to Freshwater Teleost Fish Channa Punctatus (Bloch) Using the Micronucleus Test and Comet Assay. Arch Environ Contam Toxicol. 2010;58(1):123-30. PubMed PMID: 19557474.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Investigation of the genotoxicity of malathion to freshwater teleost fish Channa punctatus (Bloch) using the micronucleus test and comet assay. AU - Kumar,Ravindra, AU - Nagpure,N S, AU - Kushwaha,Basdeo, AU - Srivastava,Satish K, AU - Lakra,W S, Y1 - 2009/06/26/ PY - 2008/12/25/received PY - 2009/06/10/accepted PY - 2009/6/27/entrez PY - 2009/6/27/pubmed PY - 2010/2/25/medline SP - 123 EP - 30 JF - Archives of environmental contamination and toxicology JO - Arch Environ Contam Toxicol VL - 58 IS - 1 N2 - Malathion [S-(1,2-dicarboethoxyethyl) O, O-dimethyl phosphorodithioate] is a widely used organophosphorus insecticide throughout the world. However, limited efforts have made to study its genotoxic effect in different fish tissues. The present investigation was aimed to assess the genotoxic potential of the pesticide to the freshwater teleost fish Channa punctatus at sublethal concentrations using the micronucleus test and comet assay. Initially, the 96-h LC50 value of commercial-grade malathion (50% EC) was determined as 5.93 ppm in a semistatic system. Based on LC50, three test concentrations (viz. sublethal I, sublethal II, and sublethal III) were determined to be 1.48, 0.74, and 0.59 ppm, respectively, and the fish specimens were exposed to these concentrations. Tissue samplings were done on days 0, 1, 3, 7, 15, 22 and 29 of malathion exposure for assessment of the induction of micronuclei (MN) frequency and DNA damage. The MN formation in the peripheral blood cells was found to be significantly higher (p < 0.05) in the treated specimens at all sampling intervals compared to the control. The MN frequency reached maximum on days 3 and 7 at sublethal I and II concentrations, respectively, followed by a nonlinear decline with the progression of the experiment. Similarly, significant effects (p < 0.05) of both concentration and time of exposure were observed on DNA damage in the gill, kidney, and lymphocytes. All of the tissues exhibited a concentration-dependent increase in DNA damage up to day 3, followed by a nonlinear decrease with the duration of exposure. A comparison of the extent of DNA damage among the tissues showed the sensitivity of gill tissue to malathion. SN - 1432-0703 UR - https://www.unboundmedicine.com/medline/citation/19557474/Investigation_of_the_genotoxicity_of_malathion_to_freshwater_teleost_fish_Channa_punctatus__Bloch__using_the_micronucleus_test_and_comet_assay_ L2 - https://dx.doi.org/10.1007/s00244-009-9354-3 DB - PRIME DP - Unbound Medicine ER -