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The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds.
J Biomed Mater Res A 2010; 93(1):247-57JB

Abstract

The aim of current study is to investigate the in vitro and in vivo behavior of dental pulp stem cells (DPSCs) seeded on electrospun poly(epsilon-caprolactone) (PCL)/gelatin scaffolds with or without the addition of nano-hydroxyapatite (nHA). For the in vitro evaluation, DNA content, alkaline phosphatase (ALP) activity and osteocalcin (OC) measurement showed that the scaffolds supported DPSC adhesion, proliferation, and odontoblastic differentiation. Moreover, the presence of nHA upregulated ALP activity and promoted OC expression. Real-time PCR data confirmed these results. SEM micrographs qualitatively confirmed the proliferation and mineralization characteristics of DPSCs on both scaffolds. Subsequently, both scaffolds seeded with DPSCs were subcutaneously implanted into immunocompromised nude mice. Scaffolds with nHA but without cells were implanted as control. Histological evaluation revealed that all implants were surrounded by a thin fibrous tissue capsule without any adverse effects. The cell/scaffold composites showed obvious in vivo hard tissue formation, but there was no sign of tissue ingrowth. Further, the combination of nHA in scaffolds did upregulate the expression of specific odontogenic genes. In conclusion, the incorporation of nHA in nanofibers indeed enhanced DPSCs differentiation towards an odontoblast-like phenotype in vitro and in vivo.

Authors+Show Affiliations

School and Hospital of Stomatology, Wuhan University, Wuhan 430079, Hubei Province, People's Republic of China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19557787

Citation

Yang, Xuechao, et al. "The Performance of Dental Pulp Stem Cells On Nanofibrous PCL/gelatin/nHA Scaffolds." Journal of Biomedical Materials Research. Part A, vol. 93, no. 1, 2010, pp. 247-57.
Yang X, Yang F, Walboomers XF, et al. The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. J Biomed Mater Res A. 2010;93(1):247-57.
Yang, X., Yang, F., Walboomers, X. F., Bian, Z., Fan, M., & Jansen, J. A. (2010). The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. Journal of Biomedical Materials Research. Part A, 93(1), pp. 247-57. doi:10.1002/jbm.a.32535.
Yang X, et al. The Performance of Dental Pulp Stem Cells On Nanofibrous PCL/gelatin/nHA Scaffolds. J Biomed Mater Res A. 2010;93(1):247-57. PubMed PMID: 19557787.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - The performance of dental pulp stem cells on nanofibrous PCL/gelatin/nHA scaffolds. AU - Yang,Xuechao, AU - Yang,Fang, AU - Walboomers,X Frank, AU - Bian,Zhuan, AU - Fan,Mingwen, AU - Jansen,John A, PY - 2009/6/27/entrez PY - 2009/6/27/pubmed PY - 2010/5/19/medline SP - 247 EP - 57 JF - Journal of biomedical materials research. Part A JO - J Biomed Mater Res A VL - 93 IS - 1 N2 - The aim of current study is to investigate the in vitro and in vivo behavior of dental pulp stem cells (DPSCs) seeded on electrospun poly(epsilon-caprolactone) (PCL)/gelatin scaffolds with or without the addition of nano-hydroxyapatite (nHA). For the in vitro evaluation, DNA content, alkaline phosphatase (ALP) activity and osteocalcin (OC) measurement showed that the scaffolds supported DPSC adhesion, proliferation, and odontoblastic differentiation. Moreover, the presence of nHA upregulated ALP activity and promoted OC expression. Real-time PCR data confirmed these results. SEM micrographs qualitatively confirmed the proliferation and mineralization characteristics of DPSCs on both scaffolds. Subsequently, both scaffolds seeded with DPSCs were subcutaneously implanted into immunocompromised nude mice. Scaffolds with nHA but without cells were implanted as control. Histological evaluation revealed that all implants were surrounded by a thin fibrous tissue capsule without any adverse effects. The cell/scaffold composites showed obvious in vivo hard tissue formation, but there was no sign of tissue ingrowth. Further, the combination of nHA in scaffolds did upregulate the expression of specific odontogenic genes. In conclusion, the incorporation of nHA in nanofibers indeed enhanced DPSCs differentiation towards an odontoblast-like phenotype in vitro and in vivo. SN - 1552-4965 UR - https://www.unboundmedicine.com/medline/citation/19557787/The_performance_of_dental_pulp_stem_cells_on_nanofibrous_PCL/gelatin/nHA_scaffolds_ L2 - https://doi.org/10.1002/jbm.a.32535 DB - PRIME DP - Unbound Medicine ER -