TY - JOUR T1 - Preparative isolation and purification of antioxidative stilbene oligomers from Vitis chunganeniss using high-speed counter-current chromatography in stepwise elution mode. AU - He,Shan, AU - Lu,Yanbin, AU - Jiang,Liyan, AU - Wu,Bin, AU - Zhang,Feiying, AU - Pan,Yuanjiang, PY - 2009/6/27/entrez PY - 2009/6/27/pubmed PY - 2009/11/17/medline SP - 2339 EP - 45 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 14 N2 - Preparative high-speed counter-current chromatography (HSCCC) was successfully applied to the isolation and purification of three stilbene oligomers from Vitis chunganeniss using stepwise elution with a pair of two-phase solvent systems composed of n-hexane-ethyl acetate-methanol-water at (2:5:2:5, v/v) and (1:2:1:2, v/v). The preparative HSCCC separation was performed on 800 mg of crude sample yielding hopeaphenol (21.1 mg), amurensin G (37.2 mg) and vitisin A (95.6 mg) in a one-step separation, with purities over 95% as determined by HPLC. The structures of these three compounds were identified by MS, (1)H NMR and (13)C NMR. In addition, their antioxidant activities were screened by DPPH assay, where vitisin A showed strong antioxidant activity. Further EPR experiments with spin-trapping technique demonstrated that vitisin A is a potent and selective singlet oxygen quencher, which may be used in singlet oxygen-mediated diseases as a pharmacological agent. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19557810/Preparative_isolation_and_purification_of_antioxidative_stilbene_oligomers_from_Vitis_chunganeniss_using_high_speed_counter_current_chromatography_in_stepwise_elution_mode_ L2 - https://doi.org/10.1002/jssc.200900033 DB - PRIME DP - Unbound Medicine ER -