Tags

Type your tag names separated by a space and hit enter

Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry.
J Forensic Sci. 1991 Sep; 36(5):1331-41.JF

Abstract

A rapid and effective solid-phase extraction procedure using Bond Elute Certify bonded silica sorbent cartridges was adopted to extract amphetamine, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) from urine samples. The extract was derivatized with trichloroacetic anhydride prior to gas chromatography/mass spectrometry (GC/MS) analysis with selected ion monitoring of the following ions: 190, 91, 188; 204, 91, 202; 162, 135, 202; 194, 123; and 211, 209 for the derivatized amphetamine, methamphetamine, MDMA, d5-amphetamine, and d9-methamphetamine, respectively. The first of the ions listed for each compound was used for quantitation. The compound d5-amphetamine was used as the internal standard for amphetamine, and d9-methamphetamine was used for methamphetamine and MDMA. Results showed a higher than 65% recovery and a reproducibility with less than a 5% coefficient of variation. When a sample size of 2 mL was used, the lowest detectable concentration was about 50 ng/mL, and a near-perfect fit can be obtained (within the 250 to 4000-ng/mL concentration range studied) using a second-order polynomial model.

Authors+Show Affiliations

Department of Criminal Justice, University of Alabama, Birmingham.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

1955829

Citation

Gan, B K., et al. "Simultaneous Analysis of Amphetamine, Methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in Urine Samples By Solid-phase Extraction, Derivatization, and Gas Chromatography/mass Spectrometry." Journal of Forensic Sciences, vol. 36, no. 5, 1991, pp. 1331-41.
Gan BK, Baugh D, Liu RH, et al. Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry. J Forensic Sci. 1991;36(5):1331-41.
Gan, B. K., Baugh, D., Liu, R. H., & Walia, A. S. (1991). Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry. Journal of Forensic Sciences, 36(5), 1331-41.
Gan BK, et al. Simultaneous Analysis of Amphetamine, Methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in Urine Samples By Solid-phase Extraction, Derivatization, and Gas Chromatography/mass Spectrometry. J Forensic Sci. 1991;36(5):1331-41. PubMed PMID: 1955829.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry. AU - Gan,B K, AU - Baugh,D, AU - Liu,R H, AU - Walia,A S, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 1331 EP - 41 JF - Journal of forensic sciences JO - J. Forensic Sci. VL - 36 IS - 5 N2 - A rapid and effective solid-phase extraction procedure using Bond Elute Certify bonded silica sorbent cartridges was adopted to extract amphetamine, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) from urine samples. The extract was derivatized with trichloroacetic anhydride prior to gas chromatography/mass spectrometry (GC/MS) analysis with selected ion monitoring of the following ions: 190, 91, 188; 204, 91, 202; 162, 135, 202; 194, 123; and 211, 209 for the derivatized amphetamine, methamphetamine, MDMA, d5-amphetamine, and d9-methamphetamine, respectively. The first of the ions listed for each compound was used for quantitation. The compound d5-amphetamine was used as the internal standard for amphetamine, and d9-methamphetamine was used for methamphetamine and MDMA. Results showed a higher than 65% recovery and a reproducibility with less than a 5% coefficient of variation. When a sample size of 2 mL was used, the lowest detectable concentration was about 50 ng/mL, and a near-perfect fit can be obtained (within the 250 to 4000-ng/mL concentration range studied) using a second-order polynomial model. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/1955829/Simultaneous_analysis_of_amphetamine_methamphetamine_and_3_4_methylenedioxymethamphetamine__MDMA__in_urine_samples_by_solid_phase_extraction_derivatization_and_gas_chromatography/mass_spectrometry_ L2 - https://medlineplus.gov/methamphetamine.html DB - PRIME DP - Unbound Medicine ER -