TY - JOUR T1 - Simultaneous analysis of amphetamine, methamphetamine, and 3, 4-methylenedioxymethamphetamine (MDMA) in urine samples by solid-phase extraction, derivatization, and gas chromatography/mass spectrometry. AU - Gan,B K, AU - Baugh,D, AU - Liu,R H, AU - Walia,A S, PY - 1991/9/1/pubmed PY - 1991/9/1/medline PY - 1991/9/1/entrez SP - 1331 EP - 41 JF - Journal of forensic sciences JO - J Forensic Sci VL - 36 IS - 5 N2 - A rapid and effective solid-phase extraction procedure using Bond Elute Certify bonded silica sorbent cartridges was adopted to extract amphetamine, methamphetamine, and 3,4-methylenedioxymethamphetamine (MDMA or Ecstasy) from urine samples. The extract was derivatized with trichloroacetic anhydride prior to gas chromatography/mass spectrometry (GC/MS) analysis with selected ion monitoring of the following ions: 190, 91, 188; 204, 91, 202; 162, 135, 202; 194, 123; and 211, 209 for the derivatized amphetamine, methamphetamine, MDMA, d5-amphetamine, and d9-methamphetamine, respectively. The first of the ions listed for each compound was used for quantitation. The compound d5-amphetamine was used as the internal standard for amphetamine, and d9-methamphetamine was used for methamphetamine and MDMA. Results showed a higher than 65% recovery and a reproducibility with less than a 5% coefficient of variation. When a sample size of 2 mL was used, the lowest detectable concentration was about 50 ng/mL, and a near-perfect fit can be obtained (within the 250 to 4000-ng/mL concentration range studied) using a second-order polynomial model. SN - 0022-1198 UR - https://www.unboundmedicine.com/medline/citation/1955829/Simultaneous_analysis_of_amphetamine_methamphetamine_and_3_4_methylenedioxymethamphetamine__MDMA__in_urine_samples_by_solid_phase_extraction_derivatization_and_gas_chromatography/mass_spectrometry_ DB - PRIME DP - Unbound Medicine ER -