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Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer.
J Chromatogr A. 2009 Oct 30; 1216(44):7533-8.JC

Abstract

A pseudo template molecularly imprinted polymer (MIP) was prepared for methotrexate (MTX) and a RP-HPLC method combined with the MIP was developed for the determination of MTX in human serum. Because of the poor solubility of MTX in common MIP preparation solvents, trimethoprim (TMP), a molecule having the similar imprinting sites as MTX, is selected as the pseudo template. The MIP was prepared using methacrylic acid (MAA) and ethylene glycol dimethacrylate as functional monomer and cross-linker, respectively. 1H NMR study showed highly strong interaction between TMP and MAA with hydrogen bonds. Chromatographic behaviors indicated that the TMP-MIP possessed excellent affinity and selectivity for MTX. And the imprinting factor for MTX was high up to 9.5 when 7:3 of acetonitrile:methanol (v/v) was used as mobile phase. Moreover, TMP-MIP was used as the solid-phase extraction (SPE) material to enrich the target compound MTX in human serum samples for HPLC analysis. The SPE process was carefully optimized and good recoveries of MTX were obtained as 81.6-86.2% with RSD of 0.22-1.84% when the spiked concentration of MTX was 2.0-10.0 microg mL(-1) in human serum samples. The results indicated that the pseudo template MIP can be applied to preconcentration, purification and analysis of MTX in clinic samples.

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Authors+Show Affiliations

Liu X
Beijing National Laboratory for Molecular Sciences, Lab of Analytical Chemistry for Life Science, Institute of Chemistry, Chinese Academy of Sciences, Beijing 100190, China.
Liu J
No affiliation info available
Huang Y
No affiliation info available
Zhao R
No affiliation info available
Liu G
No affiliation info available
Chen Y
No affiliation info available

MeSH

Chromatography, High Pressure LiquidHumansMethotrexateMolecular ImprintingPolymers

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19559443

Citation

Liu, Xiangjun, et al. "Determination of Methotrexate in Human Serum By High-performance Liquid Chromatography Combined With Pseudo Template Molecularly Imprinted Polymer." Journal of Chromatography. A, vol. 1216, no. 44, 2009, pp. 7533-8.
Liu X, Liu J, Huang Y, et al. Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer. J Chromatogr A. 2009;1216(44):7533-8.
Liu, X., Liu, J., Huang, Y., Zhao, R., Liu, G., & Chen, Y. (2009). Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer. Journal of Chromatography. A, 1216(44), 7533-8. https://doi.org/10.1016/j.chroma.2009.06.018
Liu X, et al. Determination of Methotrexate in Human Serum By High-performance Liquid Chromatography Combined With Pseudo Template Molecularly Imprinted Polymer. J Chromatogr A. 2009 Oct 30;1216(44):7533-8. PubMed PMID: 19559443.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of methotrexate in human serum by high-performance liquid chromatography combined with pseudo template molecularly imprinted polymer. AU - Liu,Xiangjun, AU - Liu,Jizhong, AU - Huang,Yanyan, AU - Zhao,Rui, AU - Liu,Guoquan, AU - Chen,Yi, Y1 - 2009/06/10/ PY - 2009/01/23/received PY - 2009/05/31/revised PY - 2009/06/03/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/12/23/medline SP - 7533 EP - 8 JF - Journal of chromatography. A JO - J Chromatogr A VL - 1216 IS - 44 N2 - A pseudo template molecularly imprinted polymer (MIP) was prepared for methotrexate (MTX) and a RP-HPLC method combined with the MIP was developed for the determination of MTX in human serum. Because of the poor solubility of MTX in common MIP preparation solvents, trimethoprim (TMP), a molecule having the similar imprinting sites as MTX, is selected as the pseudo template. The MIP was prepared using methacrylic acid (MAA) and ethylene glycol dimethacrylate as functional monomer and cross-linker, respectively. 1H NMR study showed highly strong interaction between TMP and MAA with hydrogen bonds. Chromatographic behaviors indicated that the TMP-MIP possessed excellent affinity and selectivity for MTX. And the imprinting factor for MTX was high up to 9.5 when 7:3 of acetonitrile:methanol (v/v) was used as mobile phase. Moreover, TMP-MIP was used as the solid-phase extraction (SPE) material to enrich the target compound MTX in human serum samples for HPLC analysis. The SPE process was carefully optimized and good recoveries of MTX were obtained as 81.6-86.2% with RSD of 0.22-1.84% when the spiked concentration of MTX was 2.0-10.0 microg mL(-1) in human serum samples. The results indicated that the pseudo template MIP can be applied to preconcentration, purification and analysis of MTX in clinic samples. SN - 1873-3778 UR - https://www.unboundmedicine.com/medline/citation/19559443/Determination_of_methotrexate_in_human_serum_by_high_performance_liquid_chromatography_combined_with_pseudo_template_molecularly_imprinted_polymer_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0021-9673(09)00876-0 DB - PRIME DP - Unbound Medicine ER -
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