Tags

Type your tag names separated by a space and hit enter

A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris.
Vet Microbiol. 2009 Oct 20; 139(1-2):193-6.VM

Abstract

The objective of this work was to create a fast and sensitive method of detecting Paenibacillus larvae from beehive debris based on PCR that does not require long-lasting cultivation steps. Various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into Tween 80, into water, into isopropanol and into 95% ethanol. Isolation of DNA from various spore extractions was evaluated too. Best results were provided by isolation of DNA using the QIAamp DNA Mini Kit, without heat treatment. DNA of spores was detected by PCR from 0.25 g of beeswax debris, with the detected titer of 10(5) in 1g according to the cultivation tests.

Authors+Show Affiliations

Department of Zoology, Faculty of Science, Charles University in Prague, Prague, Czech Republic. stepan.ryba@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19559547

Citation

Ryba, Stepan, et al. "A PCR Method of Detecting American Foulbrood (Paenibacillus Larvae) in Winter Beehive Wax Debris." Veterinary Microbiology, vol. 139, no. 1-2, 2009, pp. 193-6.
Ryba S, Titera D, Haklova M, et al. A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris. Vet Microbiol. 2009;139(1-2):193-6.
Ryba, S., Titera, D., Haklova, M., & Stopka, P. (2009). A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris. Veterinary Microbiology, 139(1-2), 193-6. https://doi.org/10.1016/j.vetmic.2009.05.009
Ryba S, et al. A PCR Method of Detecting American Foulbrood (Paenibacillus Larvae) in Winter Beehive Wax Debris. Vet Microbiol. 2009 Oct 20;139(1-2):193-6. PubMed PMID: 19559547.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - A PCR method of detecting American Foulbrood (Paenibacillus larvae) in winter beehive wax debris. AU - Ryba,Stepan, AU - Titera,Dalibor, AU - Haklova,Marcela, AU - Stopka,Pavel, Y1 - 2009/06/06/ PY - 2008/12/01/received PY - 2009/05/11/revised PY - 2009/05/28/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/12/16/medline SP - 193 EP - 6 JF - Veterinary microbiology JO - Vet. Microbiol. VL - 139 IS - 1-2 N2 - The objective of this work was to create a fast and sensitive method of detecting Paenibacillus larvae from beehive debris based on PCR that does not require long-lasting cultivation steps. Various methods of extracting spores from beehive debris were compared: the original method of extraction of spores into toluene, and alternative spore extraction methods into Tween 80, into water, into isopropanol and into 95% ethanol. Isolation of DNA from various spore extractions was evaluated too. Best results were provided by isolation of DNA using the QIAamp DNA Mini Kit, without heat treatment. DNA of spores was detected by PCR from 0.25 g of beeswax debris, with the detected titer of 10(5) in 1g according to the cultivation tests. SN - 1873-2542 UR - https://www.unboundmedicine.com/medline/citation/19559547/A_PCR_method_of_detecting_American_Foulbrood__Paenibacillus_larvae__in_winter_beehive_wax_debris_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-1135(09)00260-0 DB - PRIME DP - Unbound Medicine ER -