Tags

Type your tag names separated by a space and hit enter

IkB genes encoded in Cotesia plutellae bracovirus suppress an antiviral response and enhance baculovirus pathogenicity against the diamondback moth, Plutella xylostella.
J Invertebr Pathol. 2009 Sep; 102(1):79-87.JI

Abstract

An endoparasitoid wasp, Cotesia plutellae, parasitizes larvae of the diamondback moth, Plutella xylostella, with its symbiotic polydnavirus, C. plutellae bracovirus (CpBV). This study analyzed the role of Inhibitor-kB (IkB)-like genes encoded in CpBV in suppressing host antiviral response. Identified eight CpBV-IkBs are scattered on different viral genome segments and showed high homologies with other bracoviral IkBs in their amino acid sequences. Compared to an insect ortholog (e.g., Cactus of Drosophila melanogaster), they possessed a shorter ankyrin repeat domain without any regulatory domains. The eight CpBV-IkBs are, however, different in their promoter components and expression patterns in the parasitized host. To test their inhibitory activity on host antiviral response, a midgut response of P. xylostella against baculovirus infection was used as a model reaction. When the larvae were orally fed the virus, they exhibited melanotic responses of midgut epithelium, which increased with baculovirus dose and incubation time. Parasitized larvae exhibited a significant reduction in the midgut melanotic response, compared to nonparasitized larvae. Micro-injection of each of the four CpBV genome segments containing CpBV-IkBs into the hemocoel of nonparasitized larvae showed the gene expressions of the encoded IkBs and suppressed the midgut melanotic response in response to the baculovirus treatment. When nonparasitized larvae were orally administered with a recombinant baculovirus containing CpBV-IkB, they showed a significant reduction in midgut melanotic response and an enhanced susceptibility to the baculovirus infectivity.

Authors+Show Affiliations

Department of Bioresource Sciences, Andong National University, Andong 760-749, Republic of Korea.No affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19559708

Citation

Bae, Sungwoo, and Yonggyun Kim. "IkB Genes Encoded in Cotesia Plutellae Bracovirus Suppress an Antiviral Response and Enhance Baculovirus Pathogenicity Against the Diamondback Moth, Plutella Xylostella." Journal of Invertebrate Pathology, vol. 102, no. 1, 2009, pp. 79-87.
Bae S, Kim Y. IkB genes encoded in Cotesia plutellae bracovirus suppress an antiviral response and enhance baculovirus pathogenicity against the diamondback moth, Plutella xylostella. J Invertebr Pathol. 2009;102(1):79-87.
Bae, S., & Kim, Y. (2009). IkB genes encoded in Cotesia plutellae bracovirus suppress an antiviral response and enhance baculovirus pathogenicity against the diamondback moth, Plutella xylostella. Journal of Invertebrate Pathology, 102(1), 79-87. https://doi.org/10.1016/j.jip.2009.06.007
Bae S, Kim Y. IkB Genes Encoded in Cotesia Plutellae Bracovirus Suppress an Antiviral Response and Enhance Baculovirus Pathogenicity Against the Diamondback Moth, Plutella Xylostella. J Invertebr Pathol. 2009;102(1):79-87. PubMed PMID: 19559708.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - IkB genes encoded in Cotesia plutellae bracovirus suppress an antiviral response and enhance baculovirus pathogenicity against the diamondback moth, Plutella xylostella. AU - Bae,Sungwoo, AU - Kim,Yonggyun, Y1 - 2009/06/25/ PY - 2009/04/11/received PY - 2009/06/18/revised PY - 2009/06/22/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/10/29/medline SP - 79 EP - 87 JF - Journal of invertebrate pathology JO - J Invertebr Pathol VL - 102 IS - 1 N2 - An endoparasitoid wasp, Cotesia plutellae, parasitizes larvae of the diamondback moth, Plutella xylostella, with its symbiotic polydnavirus, C. plutellae bracovirus (CpBV). This study analyzed the role of Inhibitor-kB (IkB)-like genes encoded in CpBV in suppressing host antiviral response. Identified eight CpBV-IkBs are scattered on different viral genome segments and showed high homologies with other bracoviral IkBs in their amino acid sequences. Compared to an insect ortholog (e.g., Cactus of Drosophila melanogaster), they possessed a shorter ankyrin repeat domain without any regulatory domains. The eight CpBV-IkBs are, however, different in their promoter components and expression patterns in the parasitized host. To test their inhibitory activity on host antiviral response, a midgut response of P. xylostella against baculovirus infection was used as a model reaction. When the larvae were orally fed the virus, they exhibited melanotic responses of midgut epithelium, which increased with baculovirus dose and incubation time. Parasitized larvae exhibited a significant reduction in the midgut melanotic response, compared to nonparasitized larvae. Micro-injection of each of the four CpBV genome segments containing CpBV-IkBs into the hemocoel of nonparasitized larvae showed the gene expressions of the encoded IkBs and suppressed the midgut melanotic response in response to the baculovirus treatment. When nonparasitized larvae were orally administered with a recombinant baculovirus containing CpBV-IkB, they showed a significant reduction in midgut melanotic response and an enhanced susceptibility to the baculovirus infectivity. SN - 1096-0805 UR - https://www.unboundmedicine.com/medline/citation/19559708/IkB_genes_encoded_in_Cotesia_plutellae_bracovirus_suppress_an_antiviral_response_and_enhance_baculovirus_pathogenicity_against_the_diamondback_moth_Plutella_xylostella_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0022-2011(09)00137-2 DB - PRIME DP - Unbound Medicine ER -