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49A/G and CT60 polymorphisms of the cytotoxic T-lymphocyte-associated antigen 4 gene associated with autoimmune thyroid disease.
Hum Immunol 2009; 70(10):820-4HI

Abstract

Single nucleotide polymorphisms in the CTLA-4 gene have been suggested as genetic factors in the susceptibility to autoimmune thyroid disease (AITD). In our case-control study, patients with Graves' disease, Hashimoto's thyroiditis, and postpartum thyroiditis and control subjects have been genotyped for two A/G single nucleotide polymorphisms (49A/G and CT60) of the CTLA-4 gene. The 49A/G polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism method using the enzyme BseXI and the CT60 polymorphism by real-time polymerase chain reaction. Results were analyzed by chi(2) test and linkage disequilibrium analysis. In a comparison of frequencies of GG genotype, a significant association of 49A/G and CT60 polymorphism existed only for Graves' disease. In the 49A/G polymorphism, the frequency of GG genotype was significantly higher (p = 0.0408) compared with controls; the frequency of the CT60 polymorphism was significantly higher as well (p = 0.0213). The frequencies of AA and AG genotypes in control subjects did not significantly differ from frequencies in AITD patients for both polymorphisms. Our results may therefore lend support to the hypothesis that humoral autoimmunity is correlated with 49A/G and CT60 polymorphisms of the CTLA-4 gene.

Authors+Show Affiliations

University Medical Centre Ljubljana, Department of Nuclear Medicine, Zaloska cesta 7, SI-1525 Ljubljana, Slovenia. abicek2@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19559744

Citation

Bicek, Ajda, et al. "49A/G and CT60 Polymorphisms of the Cytotoxic T-lymphocyte-associated Antigen 4 Gene Associated With Autoimmune Thyroid Disease." Human Immunology, vol. 70, no. 10, 2009, pp. 820-4.
Bicek A, Zaletel K, Gaberscek S, et al. 49A/G and CT60 polymorphisms of the cytotoxic T-lymphocyte-associated antigen 4 gene associated with autoimmune thyroid disease. Hum Immunol. 2009;70(10):820-4.
Bicek, A., Zaletel, K., Gaberscek, S., Pirnat, E., Krhin, B., Stopar, T. G., & Hojker, S. (2009). 49A/G and CT60 polymorphisms of the cytotoxic T-lymphocyte-associated antigen 4 gene associated with autoimmune thyroid disease. Human Immunology, 70(10), pp. 820-4. doi:10.1016/j.humimm.2009.06.016.
Bicek A, et al. 49A/G and CT60 Polymorphisms of the Cytotoxic T-lymphocyte-associated Antigen 4 Gene Associated With Autoimmune Thyroid Disease. Hum Immunol. 2009;70(10):820-4. PubMed PMID: 19559744.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - 49A/G and CT60 polymorphisms of the cytotoxic T-lymphocyte-associated antigen 4 gene associated with autoimmune thyroid disease. AU - Bicek,Ajda, AU - Zaletel,Katja, AU - Gaberscek,Simona, AU - Pirnat,Edvard, AU - Krhin,Blaz, AU - Stopar,Tanja Gmeiner, AU - Hojker,Sergej, Y1 - 2009/06/25/ PY - 2009/01/08/received PY - 2009/06/15/revised PY - 2009/06/22/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/12/16/medline SP - 820 EP - 4 JF - Human immunology JO - Hum. Immunol. VL - 70 IS - 10 N2 - Single nucleotide polymorphisms in the CTLA-4 gene have been suggested as genetic factors in the susceptibility to autoimmune thyroid disease (AITD). In our case-control study, patients with Graves' disease, Hashimoto's thyroiditis, and postpartum thyroiditis and control subjects have been genotyped for two A/G single nucleotide polymorphisms (49A/G and CT60) of the CTLA-4 gene. The 49A/G polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism method using the enzyme BseXI and the CT60 polymorphism by real-time polymerase chain reaction. Results were analyzed by chi(2) test and linkage disequilibrium analysis. In a comparison of frequencies of GG genotype, a significant association of 49A/G and CT60 polymorphism existed only for Graves' disease. In the 49A/G polymorphism, the frequency of GG genotype was significantly higher (p = 0.0408) compared with controls; the frequency of the CT60 polymorphism was significantly higher as well (p = 0.0213). The frequencies of AA and AG genotypes in control subjects did not significantly differ from frequencies in AITD patients for both polymorphisms. Our results may therefore lend support to the hypothesis that humoral autoimmunity is correlated with 49A/G and CT60 polymorphisms of the CTLA-4 gene. SN - 1879-1166 UR - https://www.unboundmedicine.com/medline/citation/19559744/49A/G_and_CT60_polymorphisms_of_the_cytotoxic_T_lymphocyte_associated_antigen_4_gene_associated_with_autoimmune_thyroid_disease_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0198-8859(09)00162-1 DB - PRIME DP - Unbound Medicine ER -