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Anandamide-induced cell death: dual effects in primary rat decidual cell cultures.
Placenta. 2009 Aug; 30(8):686-92.P

Abstract

Anandamide (AEA) belongs to an emerging class of lipid mediators collectively termed "endocannabinoids". This endogenously synthesized compound has been implicated in multiple processes, mainly related to the regulation of cell growth/death. During pregnancy endometrial fibroblast-like stromal cells proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression, which appears to be associated with apoptosis. In order to study the role of this endocannabinoid in this process, the effects of AEA upon cell viability and cell death in primary rat decidual cell cultures was investigated. The results obtained demonstrated that AEA induces cell death, in a dose-dependent manner which is associated with morphological alterations, such as nuclear condensation, DNA fragmentation and upregulation of caspase-3/7 activities. Moreover, these effects were attenuated by AM251, a selective antagonist for the cannabinoid receptor CB1. High concentrations induced a dramatic effect in cell viability and morphology, though methyl-beta-cyclodextrin (MCD), a membrane cholesterol depletor completely reversed the cytotoxic effect. These findings suggest that AEA in the uterine environment may play an important role in regulating apoptosis through CB1 and thereby modulate decidual stability and regression during pregnancy. However, it cannot be discarded the hypothesis that AEA, in high concentrations, represent a deleterious factor during this complex process.

Authors+Show Affiliations

Serviço de Bioquímica, Faculdade de Farmácia, Universidade do Porto, Porto, Portugal.No affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19560819

Citation

Fonseca, B M., et al. "Anandamide-induced Cell Death: Dual Effects in Primary Rat Decidual Cell Cultures." Placenta, vol. 30, no. 8, 2009, pp. 686-92.
Fonseca BM, Correia-da-Silva G, Teixeira NA. Anandamide-induced cell death: dual effects in primary rat decidual cell cultures. Placenta. 2009;30(8):686-92.
Fonseca, B. M., Correia-da-Silva, G., & Teixeira, N. A. (2009). Anandamide-induced cell death: dual effects in primary rat decidual cell cultures. Placenta, 30(8), 686-92. https://doi.org/10.1016/j.placenta.2009.05.012
Fonseca BM, Correia-da-Silva G, Teixeira NA. Anandamide-induced Cell Death: Dual Effects in Primary Rat Decidual Cell Cultures. Placenta. 2009;30(8):686-92. PubMed PMID: 19560819.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Anandamide-induced cell death: dual effects in primary rat decidual cell cultures. AU - Fonseca,B M, AU - Correia-da-Silva,G, AU - Teixeira,N A, Y1 - 2009/06/27/ PY - 2008/12/30/received PY - 2009/05/25/revised PY - 2009/05/29/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/10/22/medline SP - 686 EP - 92 JF - Placenta JO - Placenta VL - 30 IS - 8 N2 - Anandamide (AEA) belongs to an emerging class of lipid mediators collectively termed "endocannabinoids". This endogenously synthesized compound has been implicated in multiple processes, mainly related to the regulation of cell growth/death. During pregnancy endometrial fibroblast-like stromal cells proliferate and differentiate into decidual cells, forming the decidua. After reaching its maximum development, the decidua undergoes regression, which appears to be associated with apoptosis. In order to study the role of this endocannabinoid in this process, the effects of AEA upon cell viability and cell death in primary rat decidual cell cultures was investigated. The results obtained demonstrated that AEA induces cell death, in a dose-dependent manner which is associated with morphological alterations, such as nuclear condensation, DNA fragmentation and upregulation of caspase-3/7 activities. Moreover, these effects were attenuated by AM251, a selective antagonist for the cannabinoid receptor CB1. High concentrations induced a dramatic effect in cell viability and morphology, though methyl-beta-cyclodextrin (MCD), a membrane cholesterol depletor completely reversed the cytotoxic effect. These findings suggest that AEA in the uterine environment may play an important role in regulating apoptosis through CB1 and thereby modulate decidual stability and regression during pregnancy. However, it cannot be discarded the hypothesis that AEA, in high concentrations, represent a deleterious factor during this complex process. SN - 1532-3102 UR - https://www.unboundmedicine.com/medline/citation/19560819/Anandamide_induced_cell_death:_dual_effects_in_primary_rat_decidual_cell_cultures_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0143-4004(09)00178-7 DB - PRIME DP - Unbound Medicine ER -