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Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan.
Int J Antimicrob Agents. 2009 Oct; 34(4):347-50.IJ

Abstract

This study investigated the relationship of plasmid-mediated quinolone resistance (PMQR) and aminoglycoside resistance among oxyimino-cephalosporin-resistant Escherichia coli (n=46) and Klebsiella oxytoca (n=28) clinical isolates in Japan. Seventy-three isolates appeared to produce an extended-spectrum beta-lactamase (ESBL) and one K. oxytoca isolate produced IMP-1 metallo-beta-lactamase (MBL). Polymerase chain reaction (PCR) and sequencing confirmed that eight CTX-M-9/SHV-12-producing isolates, one IMP-1-producing K. oxytoca isolate, and six ESBL-positive E. coli isolates respectively possessed PMQR genes qnrA1, qnrB6, and aac(6')-Ib-cr. All qnr-positive isolates also carried either aac(6')-Ib or aac(6')-IIc aminoglycoside acetyltransferase genes. Resistance determinants to beta-lactams, quinolones and aminoglycosides were co-transferred with a plasmid of ca. 140 kb. The qnrA1 gene was located downstream of insertion sequence ISCR1 in complex class 1 integrons. A novel qnrA1-carrying class 1 integron with the cassette arrangement aac(6')-IIc-aadA2 as well as a unique class 1 integron with bla(IMP-1)-aac(6')-IIc cassettes on the plasmid carrying qnrB6 were found in K. oxytoca isolates. We describe the identification of qnrB6 and aac(6')-Ib-cr and the close association of qnr with aac(6')-Ib and aac(6')-IIc for the first time in clinical isolates producing ESBL or MBL in Japan.

Authors+Show Affiliations

Department of Microbiology and Immunology, Graduate School of Health Sciences, Tokyo Medical and Dental University, 1-5-45 Yushima, Bunkyo-ku, Tokyo 113-8510, Japan. takashi.ode.mt@gmail.comNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19560903

Citation

Ode, Takashi, et al. "Analysis of Plasmid-mediated Multidrug Resistance in Escherichia Coli and Klebsiella Oxytoca Isolates From Clinical Specimens in Japan." International Journal of Antimicrobial Agents, vol. 34, no. 4, 2009, pp. 347-50.
Ode T, Saito R, Kumita W, et al. Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan. Int J Antimicrob Agents. 2009;34(4):347-50.
Ode, T., Saito, R., Kumita, W., Sato, K., Okugawa, S., Moriya, K., Koike, K., & Okamura, N. (2009). Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan. International Journal of Antimicrobial Agents, 34(4), 347-50. https://doi.org/10.1016/j.ijantimicag.2009.05.007
Ode T, et al. Analysis of Plasmid-mediated Multidrug Resistance in Escherichia Coli and Klebsiella Oxytoca Isolates From Clinical Specimens in Japan. Int J Antimicrob Agents. 2009;34(4):347-50. PubMed PMID: 19560903.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analysis of plasmid-mediated multidrug resistance in Escherichia coli and Klebsiella oxytoca isolates from clinical specimens in Japan. AU - Ode,Takashi, AU - Saito,Ryoichi, AU - Kumita,Wakako, AU - Sato,Kenya, AU - Okugawa,Shu, AU - Moriya,Kyoji, AU - Koike,Kazuhiko, AU - Okamura,Noboru, Y1 - 2009/06/27/ PY - 2009/03/19/received PY - 2009/04/16/revised PY - 2009/05/18/accepted PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/10/17/medline SP - 347 EP - 50 JF - International journal of antimicrobial agents JO - Int J Antimicrob Agents VL - 34 IS - 4 N2 - This study investigated the relationship of plasmid-mediated quinolone resistance (PMQR) and aminoglycoside resistance among oxyimino-cephalosporin-resistant Escherichia coli (n=46) and Klebsiella oxytoca (n=28) clinical isolates in Japan. Seventy-three isolates appeared to produce an extended-spectrum beta-lactamase (ESBL) and one K. oxytoca isolate produced IMP-1 metallo-beta-lactamase (MBL). Polymerase chain reaction (PCR) and sequencing confirmed that eight CTX-M-9/SHV-12-producing isolates, one IMP-1-producing K. oxytoca isolate, and six ESBL-positive E. coli isolates respectively possessed PMQR genes qnrA1, qnrB6, and aac(6')-Ib-cr. All qnr-positive isolates also carried either aac(6')-Ib or aac(6')-IIc aminoglycoside acetyltransferase genes. Resistance determinants to beta-lactams, quinolones and aminoglycosides were co-transferred with a plasmid of ca. 140 kb. The qnrA1 gene was located downstream of insertion sequence ISCR1 in complex class 1 integrons. A novel qnrA1-carrying class 1 integron with the cassette arrangement aac(6')-IIc-aadA2 as well as a unique class 1 integron with bla(IMP-1)-aac(6')-IIc cassettes on the plasmid carrying qnrB6 were found in K. oxytoca isolates. We describe the identification of qnrB6 and aac(6')-Ib-cr and the close association of qnr with aac(6')-Ib and aac(6')-IIc for the first time in clinical isolates producing ESBL or MBL in Japan. SN - 1872-7913 UR - https://www.unboundmedicine.com/medline/citation/19560903/Analysis_of_plasmid_mediated_multidrug_resistance_in_Escherichia_coli_and_Klebsiella_oxytoca_isolates_from_clinical_specimens_in_Japan_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0924-8579(09)00278-7 DB - PRIME DP - Unbound Medicine ER -