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Protein composition of oil bodies from mature Brassica napus seeds.
Proteomics. 2009 Jun; 9(12):3268-84.P

Abstract

Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation.

Authors+Show Affiliations

NRA, UMR 206, Chimie Biologique, Thiverval-Grignon, France. jolivet@grignon.inra.frNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19562800

Citation

Jolivet, Pascale, et al. "Protein Composition of Oil Bodies From Mature Brassica Napus Seeds." Proteomics, vol. 9, no. 12, 2009, pp. 3268-84.
Jolivet P, Boulard C, Bellamy A, et al. Protein composition of oil bodies from mature Brassica napus seeds. Proteomics. 2009;9(12):3268-84.
Jolivet, P., Boulard, C., Bellamy, A., Larré, C., Barre, M., Rogniaux, H., d'Andréa, S., Chardot, T., & Nesi, N. (2009). Protein composition of oil bodies from mature Brassica napus seeds. Proteomics, 9(12), 3268-84. https://doi.org/10.1002/pmic.200800449
Jolivet P, et al. Protein Composition of Oil Bodies From Mature Brassica Napus Seeds. Proteomics. 2009;9(12):3268-84. PubMed PMID: 19562800.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Protein composition of oil bodies from mature Brassica napus seeds. AU - Jolivet,Pascale, AU - Boulard,Céline, AU - Bellamy,Annick, AU - Larré,Colette, AU - Barre,Marion, AU - Rogniaux,Hélène, AU - d'Andréa,Sabine, AU - Chardot,Thierry, AU - Nesi,Nathalie, PY - 2009/6/30/entrez PY - 2009/6/30/pubmed PY - 2009/9/26/medline SP - 3268 EP - 84 JF - Proteomics JO - Proteomics VL - 9 IS - 12 N2 - Seed oil bodies (OBs) are intracellular particles storing lipids as food or biofuel reserves in oleaginous plants. Since Brassica napus OBs could be easily contaminated with protein bodies and/or myrosin cells, they must be purified step by step using floatation technique in order to remove non-specifically trapped proteins. An exhaustive description of the protein composition of rapeseed OBs from two double-zero varieties was achieved by a combination of proteomic and genomic tools. Genomic analysis led to the identification of sequences coding for major seed oil body proteins, including 19 oleosins, 5 steroleosins and 9 caleosins. Most of these proteins were also identified through proteomic analysis and displayed a high level of sequence conservation with their Arabidopsis thaliana counterparts. Two rapeseed oleosin orthologs appeared acetylated on their N-terminal alanine residue and both caleosins and steroleosins displayed a low level of phosphorylation. SN - 1615-9861 UR - https://www.unboundmedicine.com/medline/citation/19562800/Protein_composition_of_oil_bodies_from_mature_Brassica_napus_seeds_ L2 - https://doi.org/10.1002/pmic.200800449 DB - PRIME DP - Unbound Medicine ER -