Tags

Type your tag names separated by a space and hit enter

Acute allergic skin response as a new tool to evaluate the allergenicity of whey hydrolysates in a mouse model of orally induced cow's milk allergy.

Abstract

Hypoallergenic milk formulae are used for cow's milk allergic infants and may be a good option for infants at risk. Clinical studies have shown that the protein source or the hydrolysis methodology used may influence the effectiveness in infants stressing the importance of adequate pre-clinical testing of hypoallergenic formulae in an in vivo model of orally induced cow's milk allergy. This study was undertaken to introduce a new read-out system to measure the residual allergenicity of whey hydrolysates on both the sensitization and challenge phase of orally induced cow's milk allergy in mice. Mice were sensitized orally to whey or a partial whey hydrolysate (pWH) to measure the residual sensitizing capacity. To predict the residual allergenicity of hydrolysates, whey allergic mice were challenged in the ear with pWH, extensive whey hydrolysate or an amino acid-based formula. An acute allergic skin response (ear swelling at 1 h), whey-specific serum antibodies, and local MCP-1 concentrations were measured. In contrast to whey, oral sensitization with pWH did not result in the induction of whey-specific antibodies, although a minor residual skin response to whey was observed after challenge. Skin exposure to whey hydrolysates showed a hydrolysation dependent reduction of the acute allergic skin response in whey allergic mice. In contrast to whey, skin exposure to pWH did not enhance tissue MCP-1 levels. The acute allergic skin response in mice orally sensitized to cow's milk proteins reveals a new pre-clinical tool which might provide information about the residual sensitizing capacity of hydrolysates supporting the discussion on the use of hypoallergenic formulae in high risk children. This mouse model might be a relevant model for the screening of new hypoallergenic formulae aimed to prevent or treat cow's milk allergy.

Links

  • Publisher Full Text
  • Authors+Show Affiliations

    ,

    Division of Pharmacology and Pathophysiology, Utrecht Institute for Pharmaceutical Sciences, Utrecht University, The Netherlands. e.c.a.m.vanesch@uu.nl

    , , , , , ,

    Source

    MeSH

    Acute Disease
    Administration, Oral
    Allergens
    Animals
    Antibodies
    Cattle
    Chemokine CCL2
    Disease Models, Animal
    Female
    Humans
    Infant Formula
    Mice
    Mice, Inbred C3H
    Milk Hypersensitivity
    Milk Proteins
    Protein Hydrolysates
    Skin
    Whey Proteins

    Pub Type(s)

    Journal Article

    Language

    eng

    PubMed ID

    19563464

    Citation

    van Esch, Betty C A M., et al. "Acute Allergic Skin Response as a New Tool to Evaluate the Allergenicity of Whey Hydrolysates in a Mouse Model of Orally Induced Cow's Milk Allergy." Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, vol. 21, no. 4 Pt 2, 2010, pp. e780-6.
    van Esch BC, Schouten B, Hofman GA, et al. Acute allergic skin response as a new tool to evaluate the allergenicity of whey hydrolysates in a mouse model of orally induced cow's milk allergy. Pediatr Allergy Immunol. 2010;21(4 Pt 2):e780-6.
    van Esch, B. C., Schouten, B., Hofman, G. A., van Baalen, T., Nijkamp, F. P., Knippels, L. M., ... Garssen, J. (2010). Acute allergic skin response as a new tool to evaluate the allergenicity of whey hydrolysates in a mouse model of orally induced cow's milk allergy. Pediatric Allergy and Immunology : Official Publication of the European Society of Pediatric Allergy and Immunology, 21(4 Pt 2), pp. e780-6. doi:10.1111/j.1399-3038.2009.00924.x.
    van Esch BC, et al. Acute Allergic Skin Response as a New Tool to Evaluate the Allergenicity of Whey Hydrolysates in a Mouse Model of Orally Induced Cow's Milk Allergy. Pediatr Allergy Immunol. 2010;21(4 Pt 2):e780-6. PubMed PMID: 19563464.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Acute allergic skin response as a new tool to evaluate the allergenicity of whey hydrolysates in a mouse model of orally induced cow's milk allergy. AU - van Esch,Betty C A M, AU - Schouten,Bastiaan, AU - Hofman,Gerard A, AU - van Baalen,Ton, AU - Nijkamp,Frans P, AU - Knippels,Léon M J, AU - Willemsen,Linette E M, AU - Garssen,Johan, Y1 - 2009/06/26/ PY - 2009/7/1/entrez PY - 2009/7/1/pubmed PY - 2011/3/9/medline SP - e780 EP - 6 JF - Pediatric allergy and immunology : official publication of the European Society of Pediatric Allergy and Immunology JO - Pediatr Allergy Immunol VL - 21 IS - 4 Pt 2 N2 - Hypoallergenic milk formulae are used for cow's milk allergic infants and may be a good option for infants at risk. Clinical studies have shown that the protein source or the hydrolysis methodology used may influence the effectiveness in infants stressing the importance of adequate pre-clinical testing of hypoallergenic formulae in an in vivo model of orally induced cow's milk allergy. This study was undertaken to introduce a new read-out system to measure the residual allergenicity of whey hydrolysates on both the sensitization and challenge phase of orally induced cow's milk allergy in mice. Mice were sensitized orally to whey or a partial whey hydrolysate (pWH) to measure the residual sensitizing capacity. To predict the residual allergenicity of hydrolysates, whey allergic mice were challenged in the ear with pWH, extensive whey hydrolysate or an amino acid-based formula. An acute allergic skin response (ear swelling at 1 h), whey-specific serum antibodies, and local MCP-1 concentrations were measured. In contrast to whey, oral sensitization with pWH did not result in the induction of whey-specific antibodies, although a minor residual skin response to whey was observed after challenge. Skin exposure to whey hydrolysates showed a hydrolysation dependent reduction of the acute allergic skin response in whey allergic mice. In contrast to whey, skin exposure to pWH did not enhance tissue MCP-1 levels. The acute allergic skin response in mice orally sensitized to cow's milk proteins reveals a new pre-clinical tool which might provide information about the residual sensitizing capacity of hydrolysates supporting the discussion on the use of hypoallergenic formulae in high risk children. This mouse model might be a relevant model for the screening of new hypoallergenic formulae aimed to prevent or treat cow's milk allergy. SN - 1399-3038 UR - https://www.unboundmedicine.com/medline/citation/19563464/Acute_allergic_skin_response_as_a_new_tool_to_evaluate_the_allergenicity_of_whey_hydrolysates_in_a_mouse_model_of_orally_induced_cow's_milk_allergy_ L2 - https://doi.org/10.1111/j.1399-3038.2009.00924.x DB - PRIME DP - Unbound Medicine ER -