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Effect of Zanthoxylum schinifolium on TNF-alpha-induced vascular inflammation in human umbilical vein endothelial cells.
Vascul Pharmacol. 2009 May-Jun; 50(5-6):200-7.VP

Abstract

Pro-inflammatory cytokines induce the injury of endothelial cells in response to increases of adhesion molecules, leading to vascular inflammation and the development of atherosclerosis. In this study, we evaluated an ethanol extract of Zanthoxylum schinifolium (EZS) to determine if it inhibits the expressions of cellular adhesion molecules in human umbilical vein endothelial cells (HUVEC). When pretreatment of HUVEC with EZS, EZS suppressed the expression levels of cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-), and E-selectin induced by TNF-alpha. The adhesion of HL-60 cells to TNF-alpha-induced endothelial cells was decreased significantly in a concentration-dependent manner. Furthermore, TNF-alpha-induced MCP-1 and IL-8 mRNA expression levels were also attenuated by pretreatment with EZS. In addition, EZS suppressed TNF-alpha-induced production of reactive oxygen species (ROS). EZS inhibited NF-kappaB activation and IkappaB-alpha phosphorylation induced by TNF-alpha, subsequent degradation of IkappaB-alpha. Finally, EZS inhibited TNF-alpha-induced p38 MAPK and c-Jun N-terminal kinase (JNK) phosphorylation. Taken together, these results demonstrate that EZS suppresses vascular inflammatory process, which may be closely related to the inhibition of ROS, JNK, p38 MAPK and NF-kappaB activation in HUVEC.

Authors+Show Affiliations

Professional Graduate School of Oriental Medicine, Wonkwang University, Iksan, Jeonbuk, 570-749, Republic of Korea.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19563733

Citation

Cao, Li Hua, et al. "Effect of Zanthoxylum Schinifolium On TNF-alpha-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells." Vascular Pharmacology, vol. 50, no. 5-6, 2009, pp. 200-7.
Cao LH, Lee YJ, Kang DG, et al. Effect of Zanthoxylum schinifolium on TNF-alpha-induced vascular inflammation in human umbilical vein endothelial cells. Vascul Pharmacol. 2009;50(5-6):200-7.
Cao, L. H., Lee, Y. J., Kang, D. G., Kim, J. S., & Lee, H. S. (2009). Effect of Zanthoxylum schinifolium on TNF-alpha-induced vascular inflammation in human umbilical vein endothelial cells. Vascular Pharmacology, 50(5-6), 200-7. https://doi.org/10.1016/j.vph.2009.01.008
Cao LH, et al. Effect of Zanthoxylum Schinifolium On TNF-alpha-induced Vascular Inflammation in Human Umbilical Vein Endothelial Cells. Vascul Pharmacol. 2009 May-Jun;50(5-6):200-7. PubMed PMID: 19563733.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Effect of Zanthoxylum schinifolium on TNF-alpha-induced vascular inflammation in human umbilical vein endothelial cells. AU - Cao,Li Hua, AU - Lee,Yun Jung, AU - Kang,Dae Gill, AU - Kim,Jin Sook, AU - Lee,Ho Sub, PY - 2008/04/02/received PY - 2008/11/21/revised PY - 2009/01/26/accepted PY - 2009/7/1/entrez PY - 2009/7/1/pubmed PY - 2009/9/12/medline SP - 200 EP - 7 JF - Vascular pharmacology JO - Vascul Pharmacol VL - 50 IS - 5-6 N2 - Pro-inflammatory cytokines induce the injury of endothelial cells in response to increases of adhesion molecules, leading to vascular inflammation and the development of atherosclerosis. In this study, we evaluated an ethanol extract of Zanthoxylum schinifolium (EZS) to determine if it inhibits the expressions of cellular adhesion molecules in human umbilical vein endothelial cells (HUVEC). When pretreatment of HUVEC with EZS, EZS suppressed the expression levels of cell adhesion molecules such as vascular cell adhesion molecule-1 (VCAM-1), intercellular adhesion molecule-1 (ICAM-), and E-selectin induced by TNF-alpha. The adhesion of HL-60 cells to TNF-alpha-induced endothelial cells was decreased significantly in a concentration-dependent manner. Furthermore, TNF-alpha-induced MCP-1 and IL-8 mRNA expression levels were also attenuated by pretreatment with EZS. In addition, EZS suppressed TNF-alpha-induced production of reactive oxygen species (ROS). EZS inhibited NF-kappaB activation and IkappaB-alpha phosphorylation induced by TNF-alpha, subsequent degradation of IkappaB-alpha. Finally, EZS inhibited TNF-alpha-induced p38 MAPK and c-Jun N-terminal kinase (JNK) phosphorylation. Taken together, these results demonstrate that EZS suppresses vascular inflammatory process, which may be closely related to the inhibition of ROS, JNK, p38 MAPK and NF-kappaB activation in HUVEC. SN - 1879-3649 UR - https://www.unboundmedicine.com/medline/citation/19563733/Effect_of_Zanthoxylum_schinifolium_on_TNF_alpha_induced_vascular_inflammation_in_human_umbilical_vein_endothelial_cells_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1537-1891(09)00017-2 DB - PRIME DP - Unbound Medicine ER -