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Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin.
Anal Biochem. 2009 Oct 15; 393(2):149-54.AB

Abstract

We have developed an ultrasensitive analytical method based on surface-enhanced Raman spectroscopy (SERS) exploiting a Raman probe covalently bound to gold nanoparticles. The biological marker to be detected was adsorbed on functionalized gold nanoparticles. The capture of these nanoparticles via a biorecognition process between the marker and the immobilized receptor was demonstrated by atomic force microscopy (AFM) imaging. The vibrational fingerprints of the Raman probe on the capture substrate were followed to reveal the presence of the biological marker. The method, which was applied to reveal thrombin captured on a substrate containing antithrombin and heparin, resulted in the ability to detect marker concentrations down to the picomolar (pM) level.

Authors+Show Affiliations

Biophysics and Nanoscience Centre, CNISM, Facoltá di Scienze, Università della Tuscia, Viterbo, Italy. bizzarri@unitus.itNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19563767

Citation

Bizzarri, Anna Rita, and Salvatore Cannistraro. "Surface-enhanced Raman Spectroscopy Combined With Atomic Force Microscopy for Ultrasensitive Detection of Thrombin." Analytical Biochemistry, vol. 393, no. 2, 2009, pp. 149-54.
Bizzarri AR, Cannistraro S. Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin. Anal Biochem. 2009;393(2):149-54.
Bizzarri, A. R., & Cannistraro, S. (2009). Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin. Analytical Biochemistry, 393(2), 149-54. https://doi.org/10.1016/j.ab.2009.06.034
Bizzarri AR, Cannistraro S. Surface-enhanced Raman Spectroscopy Combined With Atomic Force Microscopy for Ultrasensitive Detection of Thrombin. Anal Biochem. 2009 Oct 15;393(2):149-54. PubMed PMID: 19563767.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Surface-enhanced Raman spectroscopy combined with atomic force microscopy for ultrasensitive detection of thrombin. AU - Bizzarri,Anna Rita, AU - Cannistraro,Salvatore, Y1 - 2009/06/27/ PY - 2009/01/07/received PY - 2009/05/07/revised PY - 2009/06/24/accepted PY - 2009/7/1/entrez PY - 2009/7/1/pubmed PY - 2009/9/26/medline SP - 149 EP - 54 JF - Analytical biochemistry JO - Anal Biochem VL - 393 IS - 2 N2 - We have developed an ultrasensitive analytical method based on surface-enhanced Raman spectroscopy (SERS) exploiting a Raman probe covalently bound to gold nanoparticles. The biological marker to be detected was adsorbed on functionalized gold nanoparticles. The capture of these nanoparticles via a biorecognition process between the marker and the immobilized receptor was demonstrated by atomic force microscopy (AFM) imaging. The vibrational fingerprints of the Raman probe on the capture substrate were followed to reveal the presence of the biological marker. The method, which was applied to reveal thrombin captured on a substrate containing antithrombin and heparin, resulted in the ability to detect marker concentrations down to the picomolar (pM) level. SN - 1096-0309 UR - https://www.unboundmedicine.com/medline/citation/19563767/Surface_enhanced_Raman_spectroscopy_combined_with_atomic_force_microscopy_for_ultrasensitive_detection_of_thrombin_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0003-2697(09)00452-7 DB - PRIME DP - Unbound Medicine ER -