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Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves.
J Integr Plant Biol. 2009 Jul; 51(7):654-62.JI

Abstract

Using pharmacological and biochemical approaches, the role of protein phosphorylation and the interrelationship between water stress-enhanced kinase activity, antioxidant enzyme activity, hydrogen peroxide (H2O2) accumulation and endogenous abscisic acid in maize (Zea mays L.) leaves were investigated. Water-stress upregulated the activities of total protein phosphorylation and Ca2+-dependent protein kinase, and the upregulation was blocked in abscisic acid-deficient vp5 mutant. Furthermore, pretreatments with a nicotinamide adenine dinucleotide phosphate oxidase inhibitor and a scavenger of H2O2 significantly reduced the increased activities of total protein kinase and Ca2+-dependent protein kinase in maize leaves exposed to water stress. Pretreatments with different protein kinase inhibitors also reduced the water stress-induced H2O2 production and the water stress-enhanced activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase. The data suggest that protein phosphorylation and H2O2 generation are required for water stress-induced antioxidant defense in maize leaves and that crosstalk between protein phosphorylation and H2O2 generation may occur.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Xu S
College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China.
Ding H
No affiliation info available
Su F
No affiliation info available
Zhang A
No affiliation info available
Jiang M
No affiliation info available

MeSH

Abscisic AcidAntioxidantsCalciumDehydrationHydrogen PeroxidePhosphorylationPlant LeavesPlant ProteinsPolyethylene GlycolsProtein Kinase InhibitorsProtein KinasesSeedlingsTime FactorsZea mays

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19566644

Citation

Xu, Shucheng, et al. "Involvement of Protein Phosphorylation in Water Stress-induced Antioxidant Defense in Maize Leaves." Journal of Integrative Plant Biology, vol. 51, no. 7, 2009, pp. 654-62.
Xu S, Ding H, Su F, et al. Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. J Integr Plant Biol. 2009;51(7):654-62.
Xu, S., Ding, H., Su, F., Zhang, A., & Jiang, M. (2009). Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. Journal of Integrative Plant Biology, 51(7), 654-62. https://doi.org/10.1111/j.1744-7909.2009.00844.x
Xu S, et al. Involvement of Protein Phosphorylation in Water Stress-induced Antioxidant Defense in Maize Leaves. J Integr Plant Biol. 2009;51(7):654-62. PubMed PMID: 19566644.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Involvement of protein phosphorylation in water stress-induced antioxidant defense in maize leaves. AU - Xu,Shucheng, AU - Ding,Haidong, AU - Su,Fengxia, AU - Zhang,Aying, AU - Jiang,Mingyi, PY - 2009/7/2/entrez PY - 2009/7/2/pubmed PY - 2009/10/21/medline SP - 654 EP - 62 JF - Journal of integrative plant biology JO - J Integr Plant Biol VL - 51 IS - 7 N2 - Using pharmacological and biochemical approaches, the role of protein phosphorylation and the interrelationship between water stress-enhanced kinase activity, antioxidant enzyme activity, hydrogen peroxide (H2O2) accumulation and endogenous abscisic acid in maize (Zea mays L.) leaves were investigated. Water-stress upregulated the activities of total protein phosphorylation and Ca2+-dependent protein kinase, and the upregulation was blocked in abscisic acid-deficient vp5 mutant. Furthermore, pretreatments with a nicotinamide adenine dinucleotide phosphate oxidase inhibitor and a scavenger of H2O2 significantly reduced the increased activities of total protein kinase and Ca2+-dependent protein kinase in maize leaves exposed to water stress. Pretreatments with different protein kinase inhibitors also reduced the water stress-induced H2O2 production and the water stress-enhanced activities of antioxidant enzymes such as superoxide dismutase, catalase, ascorbate peroxidase and glutathione reductase. The data suggest that protein phosphorylation and H2O2 generation are required for water stress-induced antioxidant defense in maize leaves and that crosstalk between protein phosphorylation and H2O2 generation may occur. SN - 1744-7909 UR - https://www.unboundmedicine.com/medline/citation/19566644/Involvement_of_protein_phosphorylation_in_water_stress_induced_antioxidant_defense_in_maize_leaves_ L2 - https://doi.org/10.1111/j.1744-7909.2009.00844.x DB - PRIME DP - Unbound Medicine ER -