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Calcitonin gene-related peptide modulates interleukin-13 in circulating cutaneous lymphocyte-associated antigen-positive T cells in patients with atopic dermatitis.
Br J Dermatol. 2009 Sep; 161(3):547-53.BJ

Abstract

BACKGROUND

Neuropeptides (NPs) may play an important role in the pathogenesis of atopic dermatitis (AD) by regulating immune responses and contributing to the cross-talk between the immune and nervous systems.

OBJECTIVES

To assess the ability of NPs to influence interleukin (IL)-13 and interferon (IFN)-gamma production and the expression of the activation marker HLA-DR in skin memory T cells [cutaneous lymphocyte-associated antigen (CLA)+ T cells] from patients with AD with severe, chronic lesions and intense pruritus, and from nonatopic controls.

METHODS

Cells were cultured in the presence and absence of different NPs, calcitonin gene-related peptide (CGRP), somatostatin (SOM) and substance P (SP). IL-13 and IFN-gamma production and HLA-DR expression were measured in both CLA+ and CLA- T-cell subsets by flow cytometry.

RESULTS

CGRP increased IL-13 production in peripheral blood mononuclear cells from patients with AD (P < 0.05), with no changes detected in the presence of SOM or SP. These patients with AD had a lower expression of CGRP receptor compared with controls (P < 0.05). Memory T cells incubated with CGRP also showed an increase in IL-13 (P < 0.05) and HLA-DR (P < 0.05) in CLA+ T cells from patients with AD compared with controls, but not in CLA- T cells. Patients with a higher production of IL-13 were those with higher total IgE and percentage of skin area involved. Furthermore, the IL-13/IFN-gamma ratio was increased in patients with AD after cells were cultured with CGRP (P < 0.05).

CONCLUSIONS

Our results suggest an immunomodulatory role of CGRP towards a Th2 pattern in CLA+ T cells, which may contribute to exacerbating clinical symptoms in patients with AD.

Authors+Show Affiliations

Research Unit for Allergic Diseases, Allergy Service, Carlos Haya Hospital, Málaga, Spain.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19566660

Citation

Antúnez, C, et al. "Calcitonin Gene-related Peptide Modulates Interleukin-13 in Circulating Cutaneous Lymphocyte-associated Antigen-positive T Cells in Patients With Atopic Dermatitis." The British Journal of Dermatology, vol. 161, no. 3, 2009, pp. 547-53.
Antúnez C, Torres MJ, López S, et al. Calcitonin gene-related peptide modulates interleukin-13 in circulating cutaneous lymphocyte-associated antigen-positive T cells in patients with atopic dermatitis. Br J Dermatol. 2009;161(3):547-53.
Antúnez, C., Torres, M. J., López, S., Rodriguez-Pena, R., Blanca, M., Mayorga, C., & Santamaría-Babi, L. F. (2009). Calcitonin gene-related peptide modulates interleukin-13 in circulating cutaneous lymphocyte-associated antigen-positive T cells in patients with atopic dermatitis. The British Journal of Dermatology, 161(3), 547-53. https://doi.org/10.1111/j.1365-2133.2009.09318.x
Antúnez C, et al. Calcitonin Gene-related Peptide Modulates Interleukin-13 in Circulating Cutaneous Lymphocyte-associated Antigen-positive T Cells in Patients With Atopic Dermatitis. Br J Dermatol. 2009;161(3):547-53. PubMed PMID: 19566660.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Calcitonin gene-related peptide modulates interleukin-13 in circulating cutaneous lymphocyte-associated antigen-positive T cells in patients with atopic dermatitis. AU - Antúnez,C, AU - Torres,M J, AU - López,S, AU - Rodriguez-Pena,R, AU - Blanca,M, AU - Mayorga,C, AU - Santamaría-Babi,L F, Y1 - 2009/06/29/ PY - 2009/7/2/entrez PY - 2009/7/2/pubmed PY - 2010/2/2/medline SP - 547 EP - 53 JF - The British journal of dermatology JO - Br. J. Dermatol. VL - 161 IS - 3 N2 - BACKGROUND: Neuropeptides (NPs) may play an important role in the pathogenesis of atopic dermatitis (AD) by regulating immune responses and contributing to the cross-talk between the immune and nervous systems. OBJECTIVES: To assess the ability of NPs to influence interleukin (IL)-13 and interferon (IFN)-gamma production and the expression of the activation marker HLA-DR in skin memory T cells [cutaneous lymphocyte-associated antigen (CLA)+ T cells] from patients with AD with severe, chronic lesions and intense pruritus, and from nonatopic controls. METHODS: Cells were cultured in the presence and absence of different NPs, calcitonin gene-related peptide (CGRP), somatostatin (SOM) and substance P (SP). IL-13 and IFN-gamma production and HLA-DR expression were measured in both CLA+ and CLA- T-cell subsets by flow cytometry. RESULTS: CGRP increased IL-13 production in peripheral blood mononuclear cells from patients with AD (P < 0.05), with no changes detected in the presence of SOM or SP. These patients with AD had a lower expression of CGRP receptor compared with controls (P < 0.05). Memory T cells incubated with CGRP also showed an increase in IL-13 (P < 0.05) and HLA-DR (P < 0.05) in CLA+ T cells from patients with AD compared with controls, but not in CLA- T cells. Patients with a higher production of IL-13 were those with higher total IgE and percentage of skin area involved. Furthermore, the IL-13/IFN-gamma ratio was increased in patients with AD after cells were cultured with CGRP (P < 0.05). CONCLUSIONS: Our results suggest an immunomodulatory role of CGRP towards a Th2 pattern in CLA+ T cells, which may contribute to exacerbating clinical symptoms in patients with AD. SN - 1365-2133 UR - https://www.unboundmedicine.com/medline/citation/19566660/Calcitonin_gene_related_peptide_modulates_interleukin_13_in_circulating_cutaneous_lymphocyte_associated_antigen_positive_T_cells_in_patients_with_atopic_dermatitis_ L2 - https://doi.org/10.1111/j.1365-2133.2009.09318.x DB - PRIME DP - Unbound Medicine ER -