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Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: a Philippine study.
Parasitol Int. 2009 Jun; 58(2):166-70.PI

Abstract

Specific mutations in the pfcrt and pfmdr1 genes have been reported to be associated with chloroquine-resistant falciparum malaria parasites worldwide. These genetic markers are considered to be useful tools for the elucidation of several aspects of the epidemiology of drug resistant malaria. In this study, Plasmodium falciparum isolates from three distinct areas of the Philippines were analyzed for drug-resistance-associated genetic mutations, and their association with the in vitro chloroquine (CQ) response. Two novel pfcrt 72-76 allelic types, CVMDT and SVMDT, were detected. The frequency of the pfcrt K76T mutation in the isolates that were successfully tested for in vitro CQ susceptibility was found to be 100% in Kalinga, 80% in Palawan, and 87% in Mindanao. The frequency of the pfmdr1 N86Y mutation was 39% in Kalinga, 35% in Palawan, and 93% in Mindanao isolates. No mutations were found at positions 1042 and 1246 of pfmdr1. However, there were no significant associations found between polymorphisms in these genes and in vitro CQ susceptibility. The results of this study indicate that mutations in pfcrt and pfmdr1 are not predictive of in vitro CQ resistance in Philippine isolates and may therefore not be suitable as molecular markers for surveillance.

Authors+Show Affiliations

Gunma University School of Health Sciences, 3-39-15 Showa-machi, Maebashi 371-8514, Japan. hatabu@health.gunma-u.ac.jpNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19567229

Citation

Hatabu, Toshimitsu, et al. "Association of Molecular Markers in Plasmodium Falciparum Crt and Mdr1 With in Vitro Chloroquine Resistance: a Philippine Study." Parasitology International, vol. 58, no. 2, 2009, pp. 166-70.
Hatabu T, Iwagami M, Kawazu S, et al. Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: a Philippine study. Parasitol Int. 2009;58(2):166-70.
Hatabu, T., Iwagami, M., Kawazu, S., Taguchi, N., Escueta, A. D., Villacorte, E. A., Rivera, P. T., & Kano, S. (2009). Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: a Philippine study. Parasitology International, 58(2), 166-70. https://doi.org/10.1016/j.parint.2009.01.010
Hatabu T, et al. Association of Molecular Markers in Plasmodium Falciparum Crt and Mdr1 With in Vitro Chloroquine Resistance: a Philippine Study. Parasitol Int. 2009;58(2):166-70. PubMed PMID: 19567229.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Association of molecular markers in Plasmodium falciparum crt and mdr1 with in vitro chloroquine resistance: a Philippine study. AU - Hatabu,Toshimitsu, AU - Iwagami,Moritoshi, AU - Kawazu,Shin-ichiro, AU - Taguchi,Nao, AU - Escueta,Aleyla D, AU - Villacorte,Elena A, AU - Rivera,Pilarita T, AU - Kano,Shigeyuki, Y1 - 2009/02/06/ PY - 2008/04/15/received PY - 2009/01/13/revised PY - 2009/01/24/accepted PY - 2009/7/2/entrez PY - 2009/7/2/pubmed PY - 2010/1/30/medline SP - 166 EP - 70 JF - Parasitology international JO - Parasitol Int VL - 58 IS - 2 N2 - Specific mutations in the pfcrt and pfmdr1 genes have been reported to be associated with chloroquine-resistant falciparum malaria parasites worldwide. These genetic markers are considered to be useful tools for the elucidation of several aspects of the epidemiology of drug resistant malaria. In this study, Plasmodium falciparum isolates from three distinct areas of the Philippines were analyzed for drug-resistance-associated genetic mutations, and their association with the in vitro chloroquine (CQ) response. Two novel pfcrt 72-76 allelic types, CVMDT and SVMDT, were detected. The frequency of the pfcrt K76T mutation in the isolates that were successfully tested for in vitro CQ susceptibility was found to be 100% in Kalinga, 80% in Palawan, and 87% in Mindanao. The frequency of the pfmdr1 N86Y mutation was 39% in Kalinga, 35% in Palawan, and 93% in Mindanao isolates. No mutations were found at positions 1042 and 1246 of pfmdr1. However, there were no significant associations found between polymorphisms in these genes and in vitro CQ susceptibility. The results of this study indicate that mutations in pfcrt and pfmdr1 are not predictive of in vitro CQ resistance in Philippine isolates and may therefore not be suitable as molecular markers for surveillance. SN - 1873-0329 UR - https://www.unboundmedicine.com/medline/citation/19567229/Association_of_molecular_markers_in_Plasmodium_falciparum_crt_and_mdr1_with_in_vitro_chloroquine_resistance:_a_Philippine_study_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S1383-5769(09)00025-7 DB - PRIME DP - Unbound Medicine ER -