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Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription.
Aquat Toxicol. 2009 Aug 13; 94(1):56-61.AT

Abstract

Chlorella vulgaris was tested to assess their toxicities in freshwater contaminated by the metal compounds of copper (Cu) and cadmium (Cd), both singly and combined. Exposure to 0.5 and 1.5 microM Cu or 1.0 and 2.0 microM Cd alone significantly decreased algal growth and chlorophyll content and increased reactive oxygen species (ROS) content. Two-way ANOVA analysis shows that the combination of these two metal compounds decreased cell growth, chlorophyll content and increased ROS content synergistically. The highest algal cell inhibition was 78.55%, the lowest levels of chl a, chl b and total-chl were 10.59%, 33.33% and 17.94% of the control, respectively. The highest increase in ROS was 9.15-fold greater than that of the control when exposed to Cu(1.5)+Cd(2.0). Real-time PCR shows that Cu and Cd reduced the transcript abundance of psbA and rbcL, but without a synergistic interaction, whereas Cu and Cd increased the transcript abundance of psaB synergistically. These results demonstrate that Cu and Cd independently inhibit PSII activity and CO(2) assimilation, but synergistically increase ROS content to disrupt chlorophyll synthesis and inhibit cell growth.

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Authors+Show Affiliations

Qian H
College of Biological and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310032, China.
Li J
No affiliation info available
Sun L
No affiliation info available
Chen W
No affiliation info available
Sheng GD
No affiliation info available
Liu W
No affiliation info available
Fu Z
No affiliation info available

MeSH

Analysis of VarianceCadmiumChlorella vulgarisChlorophyllCopperGene Expression RegulationPhotosynthesisReactive Oxygen SpeciesWater Pollutants, Chemical

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19570583

Citation

Qian, Haifeng, et al. "Combined Effect of Copper and Cadmium On Chlorella Vulgaris Growth and Photosynthesis-related Gene Transcription." Aquatic Toxicology (Amsterdam, Netherlands), vol. 94, no. 1, 2009, pp. 56-61.
Qian H, Li J, Sun L, et al. Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. Aquat Toxicol. 2009;94(1):56-61.
Qian, H., Li, J., Sun, L., Chen, W., Sheng, G. D., Liu, W., & Fu, Z. (2009). Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. Aquatic Toxicology (Amsterdam, Netherlands), 94(1), 56-61. https://doi.org/10.1016/j.aquatox.2009.05.014
Qian H, et al. Combined Effect of Copper and Cadmium On Chlorella Vulgaris Growth and Photosynthesis-related Gene Transcription. Aquat Toxicol. 2009 Aug 13;94(1):56-61. PubMed PMID: 19570583.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Combined effect of copper and cadmium on Chlorella vulgaris growth and photosynthesis-related gene transcription. AU - Qian,Haifeng, AU - Li,Jingjing, AU - Sun,Liwei, AU - Chen,Wei, AU - Sheng,G Daniel, AU - Liu,Weiping, AU - Fu,Zhengwei, Y1 - 2009/06/02/ PY - 2009/02/04/received PY - 2009/05/18/revised PY - 2009/05/27/accepted PY - 2009/7/3/entrez PY - 2009/7/3/pubmed PY - 2009/8/18/medline SP - 56 EP - 61 JF - Aquatic toxicology (Amsterdam, Netherlands) JO - Aquat Toxicol VL - 94 IS - 1 N2 - Chlorella vulgaris was tested to assess their toxicities in freshwater contaminated by the metal compounds of copper (Cu) and cadmium (Cd), both singly and combined. Exposure to 0.5 and 1.5 microM Cu or 1.0 and 2.0 microM Cd alone significantly decreased algal growth and chlorophyll content and increased reactive oxygen species (ROS) content. Two-way ANOVA analysis shows that the combination of these two metal compounds decreased cell growth, chlorophyll content and increased ROS content synergistically. The highest algal cell inhibition was 78.55%, the lowest levels of chl a, chl b and total-chl were 10.59%, 33.33% and 17.94% of the control, respectively. The highest increase in ROS was 9.15-fold greater than that of the control when exposed to Cu(1.5)+Cd(2.0). Real-time PCR shows that Cu and Cd reduced the transcript abundance of psbA and rbcL, but without a synergistic interaction, whereas Cu and Cd increased the transcript abundance of psaB synergistically. These results demonstrate that Cu and Cd independently inhibit PSII activity and CO(2) assimilation, but synergistically increase ROS content to disrupt chlorophyll synthesis and inhibit cell growth. SN - 1879-1514 UR - https://www.unboundmedicine.com/medline/citation/19570583/Combined_effect_of_copper_and_cadmium_on_Chlorella_vulgaris_growth_and_photosynthesis_related_gene_transcription_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0166-445X(09)00171-4 DB - PRIME DP - Unbound Medicine ER -