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Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration.
Biol Reprod. 2009 Nov; 81(5):814-25.BR

Abstract

Conceptus implantation involves pregnancy-specific alterations in extracellular matrix at the conceptus-maternal interface. Secreted phosphoprotein 1 (SPP1, osteopontin) is induced just before implantation and is present at the conceptus-maternal interface in mammals. In the present study, we investigated mechanisms by which SPP1 facilitates porcine conceptus and uterine luminal epithelial cell attachment. Native bovine milk and wild-type rat recombinant SPP1 stimulated trophectoderm cell migration. Bovine milk SPP1, ovine uterine SPP1, and recombinant wild-type, but not mutated, rat SPP1 promoted dose- and cation-dependent attachment of porcine trophectoderm and uterine luminal epithelial cells, which was markedly reduced in the presence of a linear Arg-Gly-Asp integrin-blocking peptide. Affinity chromatography and immunoprecipitation experiments revealed direct binding of alpha v beta 6 trophectoderm and alpha v beta 3 uterine epithelial cell integrins to SPP1. Immunofluorescence microscopy using SPP1-coated microspheres revealed colocalization of the alpha v integrin subunit and talin at focal adhesions as well as at the apical domain of trophectoderm cells. Similarly, immunofluorescence staining of implantation sites in frozen gravid uterine cross sections localized SPP1 and alpha v integrin to the apical surfaces of trophectoderm and luminal epithelium and beta 3 integrin to the apical surface of luminal epithelium. To our knowledge, the present study is the first to demonstrate functionally that SPP1 directly binds specific integrins to promote trophectoderm cell migration and attachment to luminal epithelium that may be critical to conceptus elongation and implantation.

Authors+Show Affiliations

Department of Veterinary Integrative Biosciences, Texas A&M University, College Station, Texas 77843, USA.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19571258

Citation

Erikson, David W., et al. "Secreted Phosphoprotein 1 (SPP1, Osteopontin) Binds to Integrin Alpha V Beta 6 On Porcine Trophectoderm Cells and Integrin Alpha V Beta 3 On Uterine Luminal Epithelial Cells, and Promotes Trophectoderm Cell Adhesion and Migration." Biology of Reproduction, vol. 81, no. 5, 2009, pp. 814-25.
Erikson DW, Burghardt RC, Bayless KJ, et al. Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration. Biol Reprod. 2009;81(5):814-25.
Erikson, D. W., Burghardt, R. C., Bayless, K. J., & Johnson, G. A. (2009). Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration. Biology of Reproduction, 81(5), 814-25. https://doi.org/10.1095/biolreprod.109.078600
Erikson DW, et al. Secreted Phosphoprotein 1 (SPP1, Osteopontin) Binds to Integrin Alpha V Beta 6 On Porcine Trophectoderm Cells and Integrin Alpha V Beta 3 On Uterine Luminal Epithelial Cells, and Promotes Trophectoderm Cell Adhesion and Migration. Biol Reprod. 2009;81(5):814-25. PubMed PMID: 19571258.
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TY - JOUR T1 - Secreted phosphoprotein 1 (SPP1, osteopontin) binds to integrin alpha v beta 6 on porcine trophectoderm cells and integrin alpha v beta 3 on uterine luminal epithelial cells, and promotes trophectoderm cell adhesion and migration. AU - Erikson,David W, AU - Burghardt,Robert C, AU - Bayless,Kayla J, AU - Johnson,Greg A, Y1 - 2009/07/01/ PY - 2009/7/3/entrez PY - 2009/7/3/pubmed PY - 2009/12/17/medline SP - 814 EP - 25 JF - Biology of reproduction JO - Biol. Reprod. VL - 81 IS - 5 N2 - Conceptus implantation involves pregnancy-specific alterations in extracellular matrix at the conceptus-maternal interface. Secreted phosphoprotein 1 (SPP1, osteopontin) is induced just before implantation and is present at the conceptus-maternal interface in mammals. In the present study, we investigated mechanisms by which SPP1 facilitates porcine conceptus and uterine luminal epithelial cell attachment. Native bovine milk and wild-type rat recombinant SPP1 stimulated trophectoderm cell migration. Bovine milk SPP1, ovine uterine SPP1, and recombinant wild-type, but not mutated, rat SPP1 promoted dose- and cation-dependent attachment of porcine trophectoderm and uterine luminal epithelial cells, which was markedly reduced in the presence of a linear Arg-Gly-Asp integrin-blocking peptide. Affinity chromatography and immunoprecipitation experiments revealed direct binding of alpha v beta 6 trophectoderm and alpha v beta 3 uterine epithelial cell integrins to SPP1. Immunofluorescence microscopy using SPP1-coated microspheres revealed colocalization of the alpha v integrin subunit and talin at focal adhesions as well as at the apical domain of trophectoderm cells. Similarly, immunofluorescence staining of implantation sites in frozen gravid uterine cross sections localized SPP1 and alpha v integrin to the apical surfaces of trophectoderm and luminal epithelium and beta 3 integrin to the apical surface of luminal epithelium. To our knowledge, the present study is the first to demonstrate functionally that SPP1 directly binds specific integrins to promote trophectoderm cell migration and attachment to luminal epithelium that may be critical to conceptus elongation and implantation. SN - 1529-7268 UR - https://www.unboundmedicine.com/medline/citation/19571258/Secreted_phosphoprotein_1__SPP1_osteopontin__binds_to_integrin_alpha_v_beta_6_on_porcine_trophectoderm_cells_and_integrin_alpha_v_beta_3_on_uterine_luminal_epithelial_cells_and_promotes_trophectoderm_cell_adhesion_and_migration_ L2 - https://academic.oup.com/biolreprod/article-lookup/doi/10.1095/biolreprod.109.078600 DB - PRIME DP - Unbound Medicine ER -