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Evaluation of receptor expression on immune system cells in the peripheral blood of asthmatic children undergoing food challenges.

Abstract

BACKGROUND

The role of food allergens in the induction of allergic reactions in the airways is not completely understood. The aim of the present study was to evaluate fluorocytometric assays of the peripheral blood during food challenge tests in children with asthma and food allergy.

PATIENTS AND METHODS

22 children with asthma and concomitant food allergy and 18 children with asthma without food allergy participated in the study. Oral challenge tests were performed using double-blind, placebo-controlled food challenge. Blood samples were collected before and 4 and 24 h after the challenge. CD25 and CD23 antigen expression was determined with monoclonal antibodies using a FACSCalibur flow.

RESULTS

The evaluation of the CD25+ T subpopulation and CD19+CD23+ B lymphocytes revealed statistically significant differences between the study group and the control group. In children with asthma and food allergy, the cell pool consisted (on average) of 9 +/- 2.8% of CD3+CD25+ cells before the challenge and of 10.3 +/- 3.8% (mean delta: 1.623; p = 0.01) after the provocation. However, placebo challenge did not significantly change the number of this T-lymphocyte subpopulation (mean delta: -0.121; p > 0.05). The highest increase in the CD25+ T-subpopulation expression was found in patients with respiratory reactions during the positive food challenge (mean delta: 4.065; p < 0.004).

CONCLUSIONS

An increase in CD25+ T-lymphocyte and CD23 B-lymphocyte populations after food allergen challenge may indicate their significant role in the pathogenesis of the active phase of the immunoinflammatory process in children with asthma and concomitant food allergy.

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  • Authors+Show Affiliations

    ,

    Department of Pediatric Allergology, Gastroenterology and Nutrition, Medical University of Lodz, PL-91-738 Lodz, Poland. anetkrog@poczta.onet.pl

    , ,

    Source

    MeSH

    Administration, Oral
    Adolescent
    Allergens
    Antigens, CD
    Asthma
    B-Lymphocytes
    Bronchial Provocation Tests
    Cell Separation
    Child
    Disease Progression
    Double-Blind Method
    Female
    Flow Cytometry
    Food Hypersensitivity
    Humans
    Immunization
    Male
    T-Lymphocytes

    Pub Type(s)

    Controlled Clinical Trial
    Journal Article

    Language

    eng

    PubMed ID

    19571570

    Citation

    Krogulska, Aneta, et al. "Evaluation of Receptor Expression On Immune System Cells in the Peripheral Blood of Asthmatic Children Undergoing Food Challenges." International Archives of Allergy and Immunology, vol. 150, no. 4, 2009, pp. 377-88.
    Krogulska A, Wasowska-Królikowska K, Polakowska E, et al. Evaluation of receptor expression on immune system cells in the peripheral blood of asthmatic children undergoing food challenges. Int Arch Allergy Immunol. 2009;150(4):377-88.
    Krogulska, A., Wasowska-Królikowska, K., Polakowska, E., & Chrul, S. (2009). Evaluation of receptor expression on immune system cells in the peripheral blood of asthmatic children undergoing food challenges. International Archives of Allergy and Immunology, 150(4), pp. 377-88. doi:10.1159/000226239.
    Krogulska A, et al. Evaluation of Receptor Expression On Immune System Cells in the Peripheral Blood of Asthmatic Children Undergoing Food Challenges. Int Arch Allergy Immunol. 2009;150(4):377-88. PubMed PMID: 19571570.
    * Article titles in AMA citation format should be in sentence-case
    TY - JOUR T1 - Evaluation of receptor expression on immune system cells in the peripheral blood of asthmatic children undergoing food challenges. AU - Krogulska,Aneta, AU - Wasowska-Królikowska,Krystyna, AU - Polakowska,Ewa, AU - Chrul,Slawomir, Y1 - 2009/07/01/ PY - 2008/07/07/received PY - 2009/03/06/accepted PY - 2009/7/3/entrez PY - 2009/7/3/pubmed PY - 2010/1/12/medline SP - 377 EP - 88 JF - International archives of allergy and immunology JO - Int. Arch. Allergy Immunol. VL - 150 IS - 4 N2 - BACKGROUND: The role of food allergens in the induction of allergic reactions in the airways is not completely understood. The aim of the present study was to evaluate fluorocytometric assays of the peripheral blood during food challenge tests in children with asthma and food allergy. PATIENTS AND METHODS: 22 children with asthma and concomitant food allergy and 18 children with asthma without food allergy participated in the study. Oral challenge tests were performed using double-blind, placebo-controlled food challenge. Blood samples were collected before and 4 and 24 h after the challenge. CD25 and CD23 antigen expression was determined with monoclonal antibodies using a FACSCalibur flow. RESULTS: The evaluation of the CD25+ T subpopulation and CD19+CD23+ B lymphocytes revealed statistically significant differences between the study group and the control group. In children with asthma and food allergy, the cell pool consisted (on average) of 9 +/- 2.8% of CD3+CD25+ cells before the challenge and of 10.3 +/- 3.8% (mean delta: 1.623; p = 0.01) after the provocation. However, placebo challenge did not significantly change the number of this T-lymphocyte subpopulation (mean delta: -0.121; p > 0.05). The highest increase in the CD25+ T-subpopulation expression was found in patients with respiratory reactions during the positive food challenge (mean delta: 4.065; p < 0.004). CONCLUSIONS: An increase in CD25+ T-lymphocyte and CD23 B-lymphocyte populations after food allergen challenge may indicate their significant role in the pathogenesis of the active phase of the immunoinflammatory process in children with asthma and concomitant food allergy. SN - 1423-0097 UR - https://www.unboundmedicine.com/medline/citation/19571570/Evaluation_of_receptor_expression_on_immune_system_cells_in_the_peripheral_blood_of_asthmatic_children_undergoing_food_challenges_ L2 - https://www.karger.com?DOI=10.1159/000226239 DB - PRIME DP - Unbound Medicine ER -