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Live vaccination with an H5-hemagglutinin-expressing infectious laryngotracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of the H5 subtype.
Vaccine. 2009 Aug 13; 27(37):5085-90.V

Abstract

Recently, we described an infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) recombinant, which had been attenuated by deletion of the viral dUTPase gene UL50, and abundantly expressed the hemagglutinin (HA) gene of a H5N1 type highly pathogenic avian influenza virus (HPAIV) of Vietnamese origin. In the present study, efficacy of this vectored vaccine (ILTV-DeltaUL50IH5V) against different H5 HPAIV was evaluated in 6-week-old chickens. After a single ocular immunization all animals developed HA-specific antibodies, and were protected against lethal infection not only with the homologous HPAIV isolate A/duck/Vietnam/TG24-01/2005 (H5N1, clade 1, hemagglutinin amino acid sequence identity 100%), but also with heterologous HPAIV A/swan/Germany/R65/2006 (H5N1, clade 2.2, identity 96.1%) or HPAIV A/chicken/Italy/8/98 (H5N2, identity 93.8%). No symptoms of disease were observed after challenge with the H5N1 viruses, and only 20% of H5N2 challenged animals developed minimal clinical signs. Real-time RT-PCR analyses of oropharyngeal swabs revealed limited challenge virus replication, but the almost complete absence of HPAIV RNA from cloacal swabs indicated that no generalized infections occurred. Thus, unlike several previous vectors, ILTV-DeltaUL50IH5V was able to protect chickens against different HPAIV isolates of the H5 subtype. Vaccination with HA-expressing ILTV also allowed differentiation of immunized from AIV-infected animals by serological tests for antibodies against influenza virus nucleoprotein.

Authors+Show Affiliations

Institute of Molecular Biology, Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Greifswald - Insel Riems, Germany.No affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article

Language

eng

PubMed ID

19573638

Citation

Pavlova, Sophia P., et al. "Live Vaccination With an H5-hemagglutinin-expressing Infectious Laryngotracheitis Virus Recombinant Protects Chickens Against Different Highly Pathogenic Avian Influenza Viruses of the H5 Subtype." Vaccine, vol. 27, no. 37, 2009, pp. 5085-90.
Pavlova SP, Veits J, Mettenleiter TC, et al. Live vaccination with an H5-hemagglutinin-expressing infectious laryngotracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of the H5 subtype. Vaccine. 2009;27(37):5085-90.
Pavlova, S. P., Veits, J., Mettenleiter, T. C., & Fuchs, W. (2009). Live vaccination with an H5-hemagglutinin-expressing infectious laryngotracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of the H5 subtype. Vaccine, 27(37), 5085-90. https://doi.org/10.1016/j.vaccine.2009.06.048
Pavlova SP, et al. Live Vaccination With an H5-hemagglutinin-expressing Infectious Laryngotracheitis Virus Recombinant Protects Chickens Against Different Highly Pathogenic Avian Influenza Viruses of the H5 Subtype. Vaccine. 2009 Aug 13;27(37):5085-90. PubMed PMID: 19573638.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Live vaccination with an H5-hemagglutinin-expressing infectious laryngotracheitis virus recombinant protects chickens against different highly pathogenic avian influenza viruses of the H5 subtype. AU - Pavlova,Sophia P, AU - Veits,Jutta, AU - Mettenleiter,Thomas C, AU - Fuchs,Walter, Y1 - 2009/06/30/ PY - 2009/02/11/received PY - 2009/05/05/revised PY - 2009/06/10/accepted PY - 2009/7/4/entrez PY - 2009/7/4/pubmed PY - 2009/9/11/medline SP - 5085 EP - 90 JF - Vaccine JO - Vaccine VL - 27 IS - 37 N2 - Recently, we described an infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) recombinant, which had been attenuated by deletion of the viral dUTPase gene UL50, and abundantly expressed the hemagglutinin (HA) gene of a H5N1 type highly pathogenic avian influenza virus (HPAIV) of Vietnamese origin. In the present study, efficacy of this vectored vaccine (ILTV-DeltaUL50IH5V) against different H5 HPAIV was evaluated in 6-week-old chickens. After a single ocular immunization all animals developed HA-specific antibodies, and were protected against lethal infection not only with the homologous HPAIV isolate A/duck/Vietnam/TG24-01/2005 (H5N1, clade 1, hemagglutinin amino acid sequence identity 100%), but also with heterologous HPAIV A/swan/Germany/R65/2006 (H5N1, clade 2.2, identity 96.1%) or HPAIV A/chicken/Italy/8/98 (H5N2, identity 93.8%). No symptoms of disease were observed after challenge with the H5N1 viruses, and only 20% of H5N2 challenged animals developed minimal clinical signs. Real-time RT-PCR analyses of oropharyngeal swabs revealed limited challenge virus replication, but the almost complete absence of HPAIV RNA from cloacal swabs indicated that no generalized infections occurred. Thus, unlike several previous vectors, ILTV-DeltaUL50IH5V was able to protect chickens against different HPAIV isolates of the H5 subtype. Vaccination with HA-expressing ILTV also allowed differentiation of immunized from AIV-infected animals by serological tests for antibodies against influenza virus nucleoprotein. SN - 1873-2518 UR - https://www.unboundmedicine.com/medline/citation/19573638/Live_vaccination_with_an_H5_hemagglutinin_expressing_infectious_laryngotracheitis_virus_recombinant_protects_chickens_against_different_highly_pathogenic_avian_influenza_viruses_of_the_H5_subtype_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0264-410X(09)00915-3 DB - PRIME DP - Unbound Medicine ER -