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Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC.
J Sep Sci. 2009 Aug; 32(15-16):2835-40.JS

Abstract

RP HPLC-UV-MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 microg/mL for myricetin and quercetin and 0.5 microg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5-3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1-20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process.

Authors+Show Affiliations

Department of Chemistry, University of Warsaw, Warsaw, Poland.No affiliation info availableNo affiliation info available

Pub Type(s)

Evaluation Study
Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19575378

Citation

Biesaga, Magdalena, et al. "Fast Analysis of Prominent Flavonoids in Tomato Using a Monolithic Column and Isocratic HPLC." Journal of Separation Science, vol. 32, no. 15-16, 2009, pp. 2835-40.
Biesaga M, Ochnik U, Pyrzynska K. Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC. J Sep Sci. 2009;32(15-16):2835-40.
Biesaga, M., Ochnik, U., & Pyrzynska, K. (2009). Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC. Journal of Separation Science, 32(15-16), 2835-40. https://doi.org/10.1002/jssc.200800730
Biesaga M, Ochnik U, Pyrzynska K. Fast Analysis of Prominent Flavonoids in Tomato Using a Monolithic Column and Isocratic HPLC. J Sep Sci. 2009;32(15-16):2835-40. PubMed PMID: 19575378.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Fast analysis of prominent flavonoids in tomato using a monolithic column and isocratic HPLC. AU - Biesaga,Magdalena, AU - Ochnik,Urszula, AU - Pyrzynska,Krystyna, PY - 2009/7/4/entrez PY - 2009/7/4/pubmed PY - 2009/10/23/medline SP - 2835 EP - 40 JF - Journal of separation science JO - J Sep Sci VL - 32 IS - 15-16 N2 - RP HPLC-UV-MS was optimized for simultaneous determination of several prominent flavonoids (quercetin, naringenin, naringin, myricetin, rutin and kaempferol) using a commercially available monolithic column and isocratic elution. The concentration of organic modifier (methanol and ACN) was tested in order to obtain the best resolution. The LODs for a UV detector at 254 nm were comprised between 0.1 microg/mL for myricetin and quercetin and 0.5 microg/mL for rutin, naringin and kaempferol with the precision in the range of 1.5-3.0% RSD. MS detection with multiply reaction monitoring (MRM) offers a considerable gain in selectivity as well as sensitivity LODs for flavonoids were in the range of 1-20 ng/mL. The developed method was used for monitoring the content of some flavonoids in tomatoes during their maturation process. SN - 1615-9314 UR - https://www.unboundmedicine.com/medline/citation/19575378/Fast_analysis_of_prominent_flavonoids_in_tomato_using_a_monolithic_column_and_isocratic_HPLC_ L2 - https://doi.org/10.1002/jssc.200800730 DB - PRIME DP - Unbound Medicine ER -