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[Effects of leukotriene receptor antagonists on vascular endothelial growth factor and its receptors in a sensitized rat model].
Zhonghua Jie He He Hu Xi Za Zhi. 2009 Mar; 32(3):177-81.ZJ

Abstract

OBJECTIVE

To investigate the effect of montelukast (MK) on airway inflammation and remodeling in asthmatic rats, and to explore the regulating role of MK on vascular endothelial growth factor (VEGF) and its receptors.

METHODS

Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups, a control group (n = 8), an asthmatic group (n = 8) and a MK treated group (n = 8). The rats were sensitized with ovalbumin and AL (OH3), and repeatedly exposed to aerosolized ovalbumin. Airway reactivity of the animals were measured by animal lung function meter. VEGF levels and leukotriene D(4) (LTD(4)) in serum were measured by enzyme linked-immunosorbent assay (ELISA). The pathologic changes of bronchi and the lung tissue were evaluated, and the expression of VEGF and its acceptors was analyzed with immunohistochemistry. The vascular counts and vascular smooth muscle thickness were measured by using image analysis system.

RESULTS

The bronchial provocation test showed that, in the asthmatic group, the average expiratory resistance increased remarkably. The serum levels of VEGF and LTD(4) in the asthmatic group were 31 +/- 6 and 11 +/- 4 respectively, significantly higher than those in the control group (17 +/- 5 and 6.1 +/- 0.7) respectively and in the MK group (15 +/- 4 and 9.8 +/- 1.6) respectively. (F 63.78, 39.56 all P < 0.01). Immunohistochemistry showed that, the expression of VEGF, VEGFR(1) and VEGFR(2) in the asthmatic group were increased, as compared to those in the control group and the treated group. The vascular counts were 14 +/- 2, 22 +/- 2 and 16 +/- 4 in the control, the asthmatic, and the treated groups.

CONCLUSIONS

VEGF and its receptors were over-expressed in the sensitized rat model, and involved in angiogenesis and airway remodeling. MK may be effective in reducing allergic airway inflammation and airway remodeling through VEGF and VEGFR.

Authors+Show Affiliations

Department of Respirology, the Second Affiliated Hospital of Hebei Medical University, Shijiazhuang 050000, China. yhs4546@sohu.com.cnNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

chi

PubMed ID

19575935

Citation

Yang, Hong-Shen, et al. "[Effects of Leukotriene Receptor Antagonists On Vascular Endothelial Growth Factor and Its Receptors in a Sensitized Rat Model]." Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, vol. 32, no. 3, 2009, pp. 177-81.
Yang HS, Wang L, Yang W, et al. [Effects of leukotriene receptor antagonists on vascular endothelial growth factor and its receptors in a sensitized rat model]. Zhonghua Jie He He Hu Xi Za Zhi. 2009;32(3):177-81.
Yang, H. S., Wang, L., Yang, W., Guan, J. T., Ma, J. Y., & Su, Y. (2009). [Effects of leukotriene receptor antagonists on vascular endothelial growth factor and its receptors in a sensitized rat model]. Zhonghua Jie He He Hu Xi Za Zhi = Zhonghua Jiehe He Huxi Zazhi = Chinese Journal of Tuberculosis and Respiratory Diseases, 32(3), 177-81.
Yang HS, et al. [Effects of Leukotriene Receptor Antagonists On Vascular Endothelial Growth Factor and Its Receptors in a Sensitized Rat Model]. Zhonghua Jie He He Hu Xi Za Zhi. 2009;32(3):177-81. PubMed PMID: 19575935.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - [Effects of leukotriene receptor antagonists on vascular endothelial growth factor and its receptors in a sensitized rat model]. AU - Yang,Hong-Shen, AU - Wang,Lai, AU - Yang,Wei, AU - Guan,Ji-Tao, AU - Ma,Jun-Yi, AU - Su,Yan, PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2010/8/18/medline SP - 177 EP - 81 JF - Zhonghua jie he he hu xi za zhi = Zhonghua jiehe he huxi zazhi = Chinese journal of tuberculosis and respiratory diseases JO - Zhonghua Jie He He Hu Xi Za Zhi VL - 32 IS - 3 N2 - OBJECTIVE: To investigate the effect of montelukast (MK) on airway inflammation and remodeling in asthmatic rats, and to explore the regulating role of MK on vascular endothelial growth factor (VEGF) and its receptors. METHODS: Twenty-four male Sprague-Dawley rats were randomly divided into 3 groups, a control group (n = 8), an asthmatic group (n = 8) and a MK treated group (n = 8). The rats were sensitized with ovalbumin and AL (OH3), and repeatedly exposed to aerosolized ovalbumin. Airway reactivity of the animals were measured by animal lung function meter. VEGF levels and leukotriene D(4) (LTD(4)) in serum were measured by enzyme linked-immunosorbent assay (ELISA). The pathologic changes of bronchi and the lung tissue were evaluated, and the expression of VEGF and its acceptors was analyzed with immunohistochemistry. The vascular counts and vascular smooth muscle thickness were measured by using image analysis system. RESULTS: The bronchial provocation test showed that, in the asthmatic group, the average expiratory resistance increased remarkably. The serum levels of VEGF and LTD(4) in the asthmatic group were 31 +/- 6 and 11 +/- 4 respectively, significantly higher than those in the control group (17 +/- 5 and 6.1 +/- 0.7) respectively and in the MK group (15 +/- 4 and 9.8 +/- 1.6) respectively. (F 63.78, 39.56 all P < 0.01). Immunohistochemistry showed that, the expression of VEGF, VEGFR(1) and VEGFR(2) in the asthmatic group were increased, as compared to those in the control group and the treated group. The vascular counts were 14 +/- 2, 22 +/- 2 and 16 +/- 4 in the control, the asthmatic, and the treated groups. CONCLUSIONS: VEGF and its receptors were over-expressed in the sensitized rat model, and involved in angiogenesis and airway remodeling. MK may be effective in reducing allergic airway inflammation and airway remodeling through VEGF and VEGFR. SN - 1001-0939 UR - https://www.unboundmedicine.com/medline/citation/19575935/[Effects_of_leukotriene_receptor_antagonists_on_vascular_endothelial_growth_factor_and_its_receptors_in_a_sensitized_rat_model]_ DB - PRIME DP - Unbound Medicine ER -