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Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film.
Talanta. 2009 Aug 15; 79(3):780-6.T

Abstract

Magnetic nanoparticles (Fe(3)O(4)) were synthesized by a chemical coprecipitation method. X-ray diffraction (XRD) and transmission electron microscope (TEM) were used to confirm the crystallite structure and the particle's radius. The Fe(3)O(4) nanoparticles and chitosan (CS) were mixed to form a matrix in which haemoglobin (Hb) can be immobilized for the fabrication of H(2)O(2) biosensor. The Fe(3)O(4)-CS-Hb film exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks due to the redox of Hb-heme Fe (III)/Fe (II) in a pH 7.0 phosphate buffer. The formal potential of Hb-heme Fe(III)/Fe(II) couple varied linearly with the increase of pH in the range of 4.0-10.0 with a slope of 46.5 mV pH(-1), indicating that electron transfer was accompanied with single proton transportation in the electrochemical reaction. The surface coverage of Hb immobilized on Fe(3)O(4)-CS film glassy carbon electrode was about 1.13 x 10(-10)mol cm(-2). The heterogeneous electron transfer rate constant (k(s)) was 1.04 s(-1), indicating great facilitation of the electron transfer between Hb and magnetic nanoparticles-chitosan modified electrode. The modified electrode showed excellent electrocatalytic activity toward oxygen and hydrogen peroxide reduction. The apparent Michaelis-Menten constant K(M)(app) for H(2)O(2) was estimated to be 38.1 micromol L(-1).

Authors+Show Affiliations

College of Chemistry and Chemical Engineering, Graduate University of Chinese Academy of Sciences, Beijing 100049, China.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19576445

Citation

Zheng, Na, et al. "Direct Electrochemistry and Electrocatalysis of Hemoglobin Immobilized in a Magnetic Nanoparticles-chitosan Film." Talanta, vol. 79, no. 3, 2009, pp. 780-6.
Zheng N, Zhou X, Yang W, et al. Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film. Talanta. 2009;79(3):780-6.
Zheng, N., Zhou, X., Yang, W., Li, X., & Yuan, Z. (2009). Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film. Talanta, 79(3), 780-6. https://doi.org/10.1016/j.talanta.2009.05.002
Zheng N, et al. Direct Electrochemistry and Electrocatalysis of Hemoglobin Immobilized in a Magnetic Nanoparticles-chitosan Film. Talanta. 2009 Aug 15;79(3):780-6. PubMed PMID: 19576445.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Direct electrochemistry and electrocatalysis of hemoglobin immobilized in a magnetic nanoparticles-chitosan film. AU - Zheng,Na, AU - Zhou,Xia, AU - Yang,Weiying, AU - Li,Xiangjun, AU - Yuan,Zhuobin, Y1 - 2009/05/12/ PY - 2009/03/04/received PY - 2009/04/27/revised PY - 2009/05/04/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2009/9/15/medline SP - 780 EP - 6 JF - Talanta JO - Talanta VL - 79 IS - 3 N2 - Magnetic nanoparticles (Fe(3)O(4)) were synthesized by a chemical coprecipitation method. X-ray diffraction (XRD) and transmission electron microscope (TEM) were used to confirm the crystallite structure and the particle's radius. The Fe(3)O(4) nanoparticles and chitosan (CS) were mixed to form a matrix in which haemoglobin (Hb) can be immobilized for the fabrication of H(2)O(2) biosensor. The Fe(3)O(4)-CS-Hb film exhibited a pair of well-defined and quasi-reversible cyclic voltammetric peaks due to the redox of Hb-heme Fe (III)/Fe (II) in a pH 7.0 phosphate buffer. The formal potential of Hb-heme Fe(III)/Fe(II) couple varied linearly with the increase of pH in the range of 4.0-10.0 with a slope of 46.5 mV pH(-1), indicating that electron transfer was accompanied with single proton transportation in the electrochemical reaction. The surface coverage of Hb immobilized on Fe(3)O(4)-CS film glassy carbon electrode was about 1.13 x 10(-10)mol cm(-2). The heterogeneous electron transfer rate constant (k(s)) was 1.04 s(-1), indicating great facilitation of the electron transfer between Hb and magnetic nanoparticles-chitosan modified electrode. The modified electrode showed excellent electrocatalytic activity toward oxygen and hydrogen peroxide reduction. The apparent Michaelis-Menten constant K(M)(app) for H(2)O(2) was estimated to be 38.1 micromol L(-1). SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19576445/Direct_electrochemistry_and_electrocatalysis_of_hemoglobin_immobilized_in_a_magnetic_nanoparticles_chitosan_film_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0039-9140(09)00374-9 DB - PRIME DP - Unbound Medicine ER -