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Determination of tetracyclines in food samples by molecularly imprinted monolithic column coupling with high performance liquid chromatography.
Talanta. 2009 Aug 15; 79(3):926-34.T

Abstract

A novel solid phase extraction (SPE) method for determination of tetracyclines (TCs) in milk and honey samples by molecularly imprinted monolithic column was developed. Using tetracycline (TC) as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, methanol as the solvent, cyclohexanol and dodecanol as the mixed porogenic solvents, a TC imprinted monolithic column was prepared by in situ molecular imprinting technique for the first time, and the optimal synthesis conditions and the selectivity of TC imprinted monolithic column were investigated. The interfering substances in food samples and TCs can be separated successfully on imprinted column. Molecularly imprinted solid phase extraction (MISPE) coupling with C18 column was used to determinate the TCs in milk and honey. The recoveries of this method for six tetracyclines antibiotics such as tetracycline (TC), oxytetracycline (OTC), minocycline (MINO), chlortetracycline (CTC), metacycline (MTC) and doxycycline (DTC) were investigated, and high recoveries of 73.3-90.6% from milk samples and 62.6-82.3% from honey samples were obtained. A method for determination of TCs at low concentration level in milk and honey samples was successfully developed by using the monolithic column as the precolumn for solid phase extraction of six TCs compounds.

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Publisher Full Text (DOI)

Authors+Show Affiliations

Sun X
Department of Chemistry, Nankai University, Tianjin 300071, PR China. xianglisun@mail.nankai.edu.cn
He X
No affiliation info available
Zhang Y
No affiliation info available
Chen L
No affiliation info available

MeSH

AnimalsAnti-Bacterial AgentsChromatography, High Pressure LiquidFood AnalysisHoneyMethacrylatesMethanolMilkMolecular ImprintingSolid Phase ExtractionSolventsTetracyclineTime Factors

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19576466

Citation

Sun, Xiangli, et al. "Determination of Tetracyclines in Food Samples By Molecularly Imprinted Monolithic Column Coupling With High Performance Liquid Chromatography." Talanta, vol. 79, no. 3, 2009, pp. 926-34.
Sun X, He X, Zhang Y, et al. Determination of tetracyclines in food samples by molecularly imprinted monolithic column coupling with high performance liquid chromatography. Talanta. 2009;79(3):926-34.
Sun, X., He, X., Zhang, Y., & Chen, L. (2009). Determination of tetracyclines in food samples by molecularly imprinted monolithic column coupling with high performance liquid chromatography. Talanta, 79(3), 926-34. https://doi.org/10.1016/j.talanta.2009.05.033
Sun X, et al. Determination of Tetracyclines in Food Samples By Molecularly Imprinted Monolithic Column Coupling With High Performance Liquid Chromatography. Talanta. 2009 Aug 15;79(3):926-34. PubMed PMID: 19576466.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Determination of tetracyclines in food samples by molecularly imprinted monolithic column coupling with high performance liquid chromatography. AU - Sun,Xiangli, AU - He,Xiwen, AU - Zhang,Yukui, AU - Chen,Langxing, Y1 - 2009/05/27/ PY - 2009/01/05/received PY - 2009/05/15/revised PY - 2009/05/19/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2009/9/15/medline SP - 926 EP - 34 JF - Talanta JO - Talanta VL - 79 IS - 3 N2 - A novel solid phase extraction (SPE) method for determination of tetracyclines (TCs) in milk and honey samples by molecularly imprinted monolithic column was developed. Using tetracycline (TC) as the template, methacrylic acid (MAA) as the functional monomer, ethylene glycol dimethacrylate (EGDMA) as the cross-linker, methanol as the solvent, cyclohexanol and dodecanol as the mixed porogenic solvents, a TC imprinted monolithic column was prepared by in situ molecular imprinting technique for the first time, and the optimal synthesis conditions and the selectivity of TC imprinted monolithic column were investigated. The interfering substances in food samples and TCs can be separated successfully on imprinted column. Molecularly imprinted solid phase extraction (MISPE) coupling with C18 column was used to determinate the TCs in milk and honey. The recoveries of this method for six tetracyclines antibiotics such as tetracycline (TC), oxytetracycline (OTC), minocycline (MINO), chlortetracycline (CTC), metacycline (MTC) and doxycycline (DTC) were investigated, and high recoveries of 73.3-90.6% from milk samples and 62.6-82.3% from honey samples were obtained. A method for determination of TCs at low concentration level in milk and honey samples was successfully developed by using the monolithic column as the precolumn for solid phase extraction of six TCs compounds. SN - 1873-3573 UR - https://www.unboundmedicine.com/medline/citation/19576466/Determination_of_tetracyclines_in_food_samples_by_molecularly_imprinted_monolithic_column_coupling_with_high_performance_liquid_chromatography_ DB - PRIME DP - Unbound Medicine ER -