Tags

Type your tag names separated by a space and hit enter

Phytase supplementation increases bone mineral density, lean body mass and voluntary physical activity in rats fed a low-zinc diet.
J Nutr Biochem. 2010 Jul; 21(7):653-8.JN

Abstract

Phytic acid forms insoluble complexes with nutritionally essential minerals, including zinc (Zn). Animal studies show that addition of microbial phytase (P) to low-Zn diets improves Zn status and bone strength. The present study determined the effects of phytase supplementation on bone mineral density (BMD), body composition and voluntary running activity of male rats fed a high phytic acid, low-Zn diet. In a factorial design, rats were assigned to ZnLO (5 mg/kg diet), ZnLO+P (ZnLO diet with 1500 U phytase/kg) or ZnAD (30 mg/kg diet) groups and were divided into voluntary exercise (EX) or sedentary (SED) groups, for 9 weeks. SED rats were significantly heavier from the second week, and no catch-up growth occurred in EX rats. Feed intakes were not different between groups throughout the study. ZnLO animals had decreased food efficiency ratios compared to both phytase-supplemented (ZnLO+P) and Zn-adequate (ZnAD) animals (P<.01 compared to ZnLO). The ZnLO+P and ZnAD rats ran 56-75 km more total distance than ZnLO rats (P<.05), with the ZnLO+P rats running more kilometers per week than the ZnLO rats by Week 6. In vivo DEXA analyses indicate that rats fed phytase-supplemented diets had higher lean body mass (LBM) than those fed ZnLO diets; and that rats fed the Zn-adequate diets had the highest LBM. Body fat (%) was significantly lower in EX rats and was both Zn- and phytase insensitive. Rats fed phytase-supplemented diets had higher bone mineral content (BMC), bone area (BA) and BMD than rats fed ZnLO diets; and in rats fed ZnAD diets these indices were the highest. The dietary effects on BMC, BA and BMD were independent of activity level. We conclude that consuming supplemental dietary phytase or dietary Zn additively enhances Zn status to increase BMD, LBM and voluntary physical activity in rats fed a low-Zn diet. While the findings confirm that bone health is vulnerable to disruption by moderate Zn deficiency in rats, this new data suggests that if dietary Zn is limiting, supplemental phytase may have beneficial effects on LBM and performance activity.

Authors+Show Affiliations

Military Nutrition Division, US Army Research Institute of Environmental Medicine, Natick, MA 01760, USA. angus.scrimgeour@us.army.milNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, U.S. Gov't, Non-P.H.S.

Language

eng

PubMed ID

19576752

Citation

Scrimgeour, Angus G., et al. "Phytase Supplementation Increases Bone Mineral Density, Lean Body Mass and Voluntary Physical Activity in Rats Fed a Low-zinc Diet." The Journal of Nutritional Biochemistry, vol. 21, no. 7, 2010, pp. 653-8.
Scrimgeour AG, Marchitelli LJ, Whicker JS, et al. Phytase supplementation increases bone mineral density, lean body mass and voluntary physical activity in rats fed a low-zinc diet. J Nutr Biochem. 2010;21(7):653-8.
Scrimgeour, A. G., Marchitelli, L. J., Whicker, J. S., Song, Y., Ho, E., & Young, A. J. (2010). Phytase supplementation increases bone mineral density, lean body mass and voluntary physical activity in rats fed a low-zinc diet. The Journal of Nutritional Biochemistry, 21(7), 653-8. https://doi.org/10.1016/j.jnutbio.2009.03.015
Scrimgeour AG, et al. Phytase Supplementation Increases Bone Mineral Density, Lean Body Mass and Voluntary Physical Activity in Rats Fed a Low-zinc Diet. J Nutr Biochem. 2010;21(7):653-8. PubMed PMID: 19576752.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Phytase supplementation increases bone mineral density, lean body mass and voluntary physical activity in rats fed a low-zinc diet. AU - Scrimgeour,Angus G, AU - Marchitelli,Louis J, AU - Whicker,Jered S, AU - Song,Yang, AU - Ho,Emily, AU - Young,Andrew J, Y1 - 2009/07/02/ PY - 2008/09/05/received PY - 2009/02/27/revised PY - 2009/03/23/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2010/10/6/medline SP - 653 EP - 8 JF - The Journal of nutritional biochemistry JO - J. Nutr. Biochem. VL - 21 IS - 7 N2 - Phytic acid forms insoluble complexes with nutritionally essential minerals, including zinc (Zn). Animal studies show that addition of microbial phytase (P) to low-Zn diets improves Zn status and bone strength. The present study determined the effects of phytase supplementation on bone mineral density (BMD), body composition and voluntary running activity of male rats fed a high phytic acid, low-Zn diet. In a factorial design, rats were assigned to ZnLO (5 mg/kg diet), ZnLO+P (ZnLO diet with 1500 U phytase/kg) or ZnAD (30 mg/kg diet) groups and were divided into voluntary exercise (EX) or sedentary (SED) groups, for 9 weeks. SED rats were significantly heavier from the second week, and no catch-up growth occurred in EX rats. Feed intakes were not different between groups throughout the study. ZnLO animals had decreased food efficiency ratios compared to both phytase-supplemented (ZnLO+P) and Zn-adequate (ZnAD) animals (P<.01 compared to ZnLO). The ZnLO+P and ZnAD rats ran 56-75 km more total distance than ZnLO rats (P<.05), with the ZnLO+P rats running more kilometers per week than the ZnLO rats by Week 6. In vivo DEXA analyses indicate that rats fed phytase-supplemented diets had higher lean body mass (LBM) than those fed ZnLO diets; and that rats fed the Zn-adequate diets had the highest LBM. Body fat (%) was significantly lower in EX rats and was both Zn- and phytase insensitive. Rats fed phytase-supplemented diets had higher bone mineral content (BMC), bone area (BA) and BMD than rats fed ZnLO diets; and in rats fed ZnAD diets these indices were the highest. The dietary effects on BMC, BA and BMD were independent of activity level. We conclude that consuming supplemental dietary phytase or dietary Zn additively enhances Zn status to increase BMD, LBM and voluntary physical activity in rats fed a low-Zn diet. While the findings confirm that bone health is vulnerable to disruption by moderate Zn deficiency in rats, this new data suggests that if dietary Zn is limiting, supplemental phytase may have beneficial effects on LBM and performance activity. SN - 1873-4847 UR - https://www.unboundmedicine.com/medline/citation/19576752/Phytase_supplementation_increases_bone_mineral_density_lean_body_mass_and_voluntary_physical_activity_in_rats_fed_a_low_zinc_diet_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0955-2863(09)00084-9 DB - PRIME DP - Unbound Medicine ER -