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Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice.
J Ethnopharmacol. 2009 Sep 07; 125(2):297-303.JE

Abstract

AIMS OF THE STUDY

This study investigated the anti-inflammatory and analgesic activities, and protoberberine alkaloid contents of ethanol extract of MO roots (MOR(EtOH)).

MATERIALS AND METHODS

The analgesic activity of MOR(EtOH) was determined using acetic acid-induced writhing response and formalin test. The anti-inflammatory activity of MOR(EtOH) was determined using the lambda-carrageenan-induced paw oedema model. The protoberberine alkaloid contents of MOR(EtOH) were identified by high-performance liquid chromatography (HPLC).

RESULTS

MOR(EtOH) (100 and 500 mg/kg) decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, carrageenan-induced paw oedema was significantly reduced in a dose-dependent manner by administering MOR(EtOH) (100 and 500 mg/kg) at 3, 4, and 5h after the carrageenan injection. The serum levels of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) of MOR(EtOH)-treated mice were significantly reduced compared with those in the serum of animals administered carrageenan. Notably, MOR(EtOH) attenuated the expression of cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) and neutrophil infiltration in paw tissues injected with carrageenan. The anti-inflammatory mechanisms of MOR(EtOH) appear to be related to the inhibition of neutrophil infiltration, iNOS and COX-2 protein expression, NO release, and the decreasing TNF-alpha level in serum. The analytical results showed that the contents of berberine, palmatine and jatrorrhizine were 191.45 mg/g extract, 100.15 mg/g extract and 66.45 mg/g extract, respectively.

CONCLUSION

These experimental results suggest that MOR(EtOH) produced both analgesic and anti-inflammatory effects in mice and may be a candidate for the development of pharmacological agents used in the treatment of inflammatory disorders.

Authors+Show Affiliations

Graduate Institute of Chinese Pharmaceutical Sciences, College of Pharmacy, China Medical University, Taichung, Taiwan.No affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info availableNo affiliation info available

Pub Type(s)

Journal Article
Research Support, Non-U.S. Gov't

Language

eng

PubMed ID

19576980

Citation

Chao, Jung, et al. "Analgesic and Anti-inflammatory Activities of Ethanol Root Extract of Mahonia Oiwakensis in Mice." Journal of Ethnopharmacology, vol. 125, no. 2, 2009, pp. 297-303.
Chao J, Lu TC, Liao JW, et al. Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice. J Ethnopharmacol. 2009;125(2):297-303.
Chao, J., Lu, T. C., Liao, J. W., Huang, T. H., Lee, M. S., Cheng, H. Y., Ho, L. K., Kuo, C. L., & Peng, W. H. (2009). Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice. Journal of Ethnopharmacology, 125(2), 297-303. https://doi.org/10.1016/j.jep.2009.06.024
Chao J, et al. Analgesic and Anti-inflammatory Activities of Ethanol Root Extract of Mahonia Oiwakensis in Mice. J Ethnopharmacol. 2009 Sep 7;125(2):297-303. PubMed PMID: 19576980.
* Article titles in AMA citation format should be in sentence-case
TY - JOUR T1 - Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice. AU - Chao,Jung, AU - Lu,Tsung-Chun, AU - Liao,Jiunn-Wang, AU - Huang,Tai-Hung, AU - Lee,Meng-Shiou, AU - Cheng,Hao-Yuan, AU - Ho,Li-Kang, AU - Kuo,Chao-Lin, AU - Peng,Wen-Huang, Y1 - 2009/07/02/ PY - 2009/03/10/received PY - 2009/06/18/revised PY - 2009/06/22/accepted PY - 2009/7/7/entrez PY - 2009/7/7/pubmed PY - 2009/12/16/medline SP - 297 EP - 303 JF - Journal of ethnopharmacology JO - J Ethnopharmacol VL - 125 IS - 2 N2 - AIMS OF THE STUDY: This study investigated the anti-inflammatory and analgesic activities, and protoberberine alkaloid contents of ethanol extract of MO roots (MOR(EtOH)). MATERIALS AND METHODS: The analgesic activity of MOR(EtOH) was determined using acetic acid-induced writhing response and formalin test. The anti-inflammatory activity of MOR(EtOH) was determined using the lambda-carrageenan-induced paw oedema model. The protoberberine alkaloid contents of MOR(EtOH) were identified by high-performance liquid chromatography (HPLC). RESULTS: MOR(EtOH) (100 and 500 mg/kg) decreased the acetic acid-induced writhing responses and licking times of the second phase in the formalin test. Moreover, carrageenan-induced paw oedema was significantly reduced in a dose-dependent manner by administering MOR(EtOH) (100 and 500 mg/kg) at 3, 4, and 5h after the carrageenan injection. The serum levels of tumor necrosis factor-alpha (TNF-alpha) and nitric oxide (NO) of MOR(EtOH)-treated mice were significantly reduced compared with those in the serum of animals administered carrageenan. Notably, MOR(EtOH) attenuated the expression of cyclo-oxygenase 2 (COX-2) and inducible nitric oxide synthase (iNOS) and neutrophil infiltration in paw tissues injected with carrageenan. The anti-inflammatory mechanisms of MOR(EtOH) appear to be related to the inhibition of neutrophil infiltration, iNOS and COX-2 protein expression, NO release, and the decreasing TNF-alpha level in serum. The analytical results showed that the contents of berberine, palmatine and jatrorrhizine were 191.45 mg/g extract, 100.15 mg/g extract and 66.45 mg/g extract, respectively. CONCLUSION: These experimental results suggest that MOR(EtOH) produced both analgesic and anti-inflammatory effects in mice and may be a candidate for the development of pharmacological agents used in the treatment of inflammatory disorders. SN - 1872-7573 UR - https://www.unboundmedicine.com/medline/citation/19576980/Analgesic_and_anti_inflammatory_activities_of_ethanol_root_extract_of_Mahonia_oiwakensis_in_mice_ L2 - https://linkinghub.elsevier.com/retrieve/pii/S0378-8741(09)00399-7 DB - PRIME DP - Unbound Medicine ER -